EK3220S

Rat Insulin Competitive Standard (大鼠胰岛素竞争法 标准品)

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¥180.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:INS, Insulin
  • 种属:大鼠 (Rat)
  • 试剂盒:EK3220
  • 保存:短期4℃保存,长期-20℃保存
  • 运输条件:4℃蓝冰运输

在售SKU:70-EK3220S


描述

本产品只包含标准品试剂,如需购买试剂盒请点击下图

商品名

Rat Insulin Competitive Standard (大鼠胰岛素竞争法 标准品)

组分

Rat Insulin冻干标准品

检测方法

竞争法

样本类型

血清,血浆,细胞培养上清及其他生物学样本

板式

保存

短期4℃,长期-20℃保存

运输条件

4℃蓝冰运输

检测原理:本试剂盒采用固相竞争酶联免疫吸附检测技术。特异性抗大鼠Insulin抗体预包被在高亲和力的酶标板上。酶标板孔中加入生物素化的Insulin和未标记的Insulin或样本,竞争结合包被抗体上有限的结合位点。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中Insulin的浓度成反比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Rat Insulin Competitive ELISA Kit (大鼠胰岛素竞争法 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述大鼠 INS, Insulin 靶点信息

INS 分子靶点信息概述

  • 分子名:INS, insulin
  • 基因家族:Neuropeptides
  • 曾用名:IDDM2; IDDM1
  • 全称:insulin-dependent diabetes mellitus 2

INS 分子靶点综述

胰岛素是一种由胰岛β细胞和一些硬骨鱼布氏体分泌产生的肽类激素。它通过促进脂肪、肝脏和骨骼肌细胞吸收血液中的葡萄糖,从而对糖类、脂肪和蛋白质代谢起重要作用。这些组织吸收的葡萄糖转化为糖原或者脂肪(甘油三酯), 而肝脏是个例外,其吸收的葡萄糖可以转化为糖原和脂肪。肝脏中的葡萄糖产量受血液中高浓度胰岛素强烈抑制。血液中胰岛素浓度高时,它就是一种同化激素,可促进血液中的小分子转化为细胞内的大分子。血液中胰岛素浓度低时,它通过促进广泛的分解代谢而产生相反的效果。当胰岛β细胞被自身免疫过程破坏,胰岛素就不再合成或者分泌到血液中,进而导致 1 型糖尿病。遗传易感性和环境因素的综合影响会导致胰岛素抵抗,这就是 2 型糖尿病。另外,胰岛β细胞产生过量的胰岛素或反应性低血糖都与胰岛素瘤有关。

大鼠 Rat Ins2 分子靶点信息

  • 分子名:Ins2, insulin 2
  • 别称:
    • C-peptide
    • CP-II
    • insulin-2
    • LOC102549619
    • uncharacterized LOC102549619
  • 基因序列:NCBI_Gene: 24506
  • 蛋白序列:UniProtKB: P01323

大鼠 Rat Ins2 靶点分子功能(预测)

Predicted to enable several functions, including identical protein binding activity; signaling receptor binding activity; and zinc ion binding activity. Predicted to be involved in several processes, including negative regulation of catabolic process; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Predicted to act upstream of or within several processes, including cell surface receptor signaling pathway; intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress; and positive regulation of phosphorylation. Located in extracellular space. Colocalizes with secretory granule. Used to study hypertension. Biomarker of Alzheimer's disease. Human ortholog(s) of this gene implicated in diabetic retinopathy; glucose metabolism disease (multiple); insulinoma; and pancreatic cancer. Orthologous to human INS (insulin).

操作步骤

ELISA操作常见问题

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Reconfiguration of Gut Microbiota and Reprogramming of Liver Metabolism with Phycobiliproteins Bioactive Peptides to Rehabilitate Obese Rats 

  2. Pi-Dan-Jian-Qing Decoction Ameliorates Type 2 Diabetes Mellitus Through Regulating the Gut Microbiota and Serum Metabolism 

  3. Fucoidan ameliorates glucose metabolism by the improvement of intestinal barrier and inflammatory damage in type 2 diabetic rats 

  4. San-Huang-Yi-Shen Capsule Ameliorates Diabetic Kidney Disease through Inducing PINK1/Parkin-Mediated Mitophagy and Inhibiting the Activation of NLRP3 Signaling Pathway 

  5. Structural modulation of gut microbiota during alleviation of non-alcoholic fatty liver disease with Gynostemma pentaphyllum in rats 

  6. Da-Chai-Hu Decoction Ameliorates High Fat Diet-Induced Nonalcoholic Fatty Liver Disease Through Remodeling the Gut Microbiota and Modulating the Serum Metabolism 

  7. Alpha-lipoic acid activates AMPK to protect against oxidative stress and apoptosis in rats with diabetic peripheral neuropathy 

  8. Hypoglycemic and hypolipidemic effects of Epigynum auritum in high fat diet and streptozotocin-induced diabetic rats 

  9. Physicochemical Characteristics and Antidiabetic Properties of the Polysaccharides from Pseudostellaria heterophylla 

  10. The self-nanoemulsifying drug delivery system of Petiveria alliacea extract reduced the homeostatic model assessment-insulin resistance value, interleukin-6, and tumor necrosis factor-α level in diabetic rat models 

  11. Serum untargeted metabolomics analysis of the mechanisms of evodiamine on type 2 diabetes mellitus model rats 

  12. Emodin Alleviates High-Glucose-Induced Pancreatic β-Cell Pyroptosis by Inhibiting NLRP3/GSDMD Signaling 

  13. Circ_005077 accelerates myocardial lipotoxicity induced by high-fat diet via CyPA/p47PHOX mediated ferroptosis 

  14. Grape Seed Proanthocyanidins Protect Pancreatic β Cells Against Ferroptosis via the Nrf2 Pathway in Type 2 Diabetes 

  15. Didymin protects pancreatic beta cells by enhancing mitochondrial function in high-fat diet-induced impaired glucose tolerance 

  16. Jianpi Shengqing Huazhuo Formula improves abnormal glucose and lipid metabolism in obesity by regulating mitochondrial biogenesis 

  17. Vitamin D3 alleviates lung fibrosis of type 2 diabetic rats via SIRT3 mediated suppression of pyroptosis 

  18. Jiang-Tang-San-Huang pill alleviates type 2 diabetes mellitus through modulating the gut microbiota and bile acids metabolism 

  19. Controlled Interfacial Polymer Self-Assembly Coordinates Ultrahigh Drug Loading and Zero-Order Release in Particles Prepared under Continuous Flow 

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