Mouse/Rat Insulin Competitive ELISA Kit 检测试剂盒(酶联免疫吸附法)
¥2,000.00 – ¥3,400.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:INS, Insulin
- 种属:大鼠 (Rat),小鼠(Mouse)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:20 μL
- 灵敏度:0.15 ng/mL
- 检测范围:0.16 ng/mL - 20 ng/mL
- 回收率:85% - 116%
在售SKU:70-EK3220-48, 70-EK3220-96, EK3220, 胰岛素
描述
商品名 |
Mouse/Rat Insulin Competitive ELISA Kit 检测试剂盒(酶联免疫吸附法)说明书 |
---|---|
检测方法 |
竞争法 |
精密度 |
板内变异系数:4.58% - 7.31%;板间变异系数:5.88% - 8.85% |
样本类型 |
血清,血浆,细胞培养上清 |
检测样本体积 |
血清,血浆:20 μL;细胞培养上清:100 μL |
灵敏度 |
0.15 ng/mL |
检测范围 |
0.16 ng/mL - 20 ng/mL |
回收率 |
85% - 116% |
平均回收率 |
1.03 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用固相竞争酶联免疫吸附检测技术。特异性抗小鼠/大鼠 Insulin 抗体预包被在高亲和力的酶标板上。酶标板孔中加入生物素化的 Insulin 和未标记的 Insulin 或样本,竞争结合包被抗体上有限的结合位点。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物 TMB,避光显色。颜色反应的深浅与 样本中 Insulin 的浓度成反比。加入终止液终止反应,在 450 nm波长(参考波长 570 - 630 nm)测定吸光度值。
分子信息
INS 分子靶点信息概述
- 分子名:INS, insulin
- 基因家族:Neuropeptides
- 曾用名:IDDM2; IDDM1
- 全称:insulin-dependent diabetes mellitus 2
INS 分子靶点综述
胰岛素是一种由胰岛β细胞和一些硬骨鱼布氏体分泌产生的肽类激素。它通过促进脂肪、肝脏和骨骼肌细胞吸收血液中的葡萄糖,从而对糖类、脂肪和蛋白质代谢起重要作用。这些组织吸收的葡萄糖转化为糖原或者脂肪(甘油三酯), 而肝脏是个例外,其吸收的葡萄糖可以转化为糖原和脂肪。肝脏中的葡萄糖产量受血液中高浓度胰岛素强烈抑制。血液中胰岛素浓度高时,它就是一种同化激素,可促进血液中的小分子转化为细胞内的大分子。血液中胰岛素浓度低时,它通过促进广泛的分解代谢而产生相反的效果。当胰岛β细胞被自身免疫过程破坏,胰岛素就不再合成或者分泌到血液中,进而导致 1 型糖尿病。遗传易感性和环境因素的综合影响会导致胰岛素抵抗,这就是 2 型糖尿病。另外,胰岛β细胞产生过量的胰岛素或反应性低血糖都与胰岛素瘤有关。
大鼠 Rat Ins2 分子靶点信息
- 分子名:Ins2, insulin 2
- 别称:
- C-peptide
- CP-II
- insulin-2
- LOC102549619
- uncharacterized LOC102549619
- 基因序列:NCBI_Gene: 24506
- 蛋白序列:UniProtKB: P01323
大鼠 Rat Ins2 靶点分子功能(预测)
Predicted to enable several functions, including identical protein binding activity; signaling receptor binding activity; and zinc ion binding activity. Predicted to be involved in several processes, including negative regulation of catabolic process; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Predicted to act upstream of or within several processes, including cell surface receptor signaling pathway; intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress; and positive regulation of phosphorylation. Located in extracellular space. Colocalizes with secretory granule. Used to study hypertension. Biomarker of Alzheimer's disease. Human ortholog(s) of this gene implicated in diabetic retinopathy; glucose metabolism disease (multiple); insulinoma; and pancreatic cancer. Orthologous to human INS (insulin).
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 血清OR血浆,哪个是ELISA的菜 
- ELISA通关必备丨数据篇丨标准曲线不佳 
- ELISA通关必备丨操作篇丨常见问题及解决方案 
- ELISA通关必备丨操作篇丨溶解与稀释标准品 
- ELISA通关必备丨样本篇丨不常见样本 
- ELISA通关必备丨样本篇丨常见样本丨细胞 
- ELISA通关必备丨如何选择试剂盒 
- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
- ELISA常见类型一 | 双抗夹心法,你要的都在这里! 
- ELISA常见类型二 | 竞争法,五分钟搞定! 
- 叮!联科向您投递了个ELISA实验操作干货包,请查收~ 
- 【视频】ELISA实验操作步骤演示视频教程 
- ELISA 组织样本的处理—大鼠组织 
- 【视频】ELISA实验原理与常见问题分析 
- 查看更多ELISA操作相关问题
ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- Reconfiguration of Gut Microbiota and Reprogramming of Liver Metabolism with Phycobiliproteins Bioactive Peptides to Rehabilitate Obese Rats 
- Pi-Dan-Jian-Qing Decoction Ameliorates Type 2 Diabetes Mellitus Through Regulating the Gut Microbiota and Serum Metabolism 
- Fucoidan ameliorates glucose metabolism by the improvement of intestinal barrier and inflammatory damage in type 2 diabetic rats 
- San-Huang-Yi-Shen Capsule Ameliorates Diabetic Kidney Disease through Inducing PINK1/Parkin-Mediated Mitophagy and Inhibiting the Activation of NLRP3 Signaling Pathway 
- Structural modulation of gut microbiota during alleviation of non-alcoholic fatty liver disease with Gynostemma pentaphyllum in rats 
- Da-Chai-Hu Decoction Ameliorates High Fat Diet-Induced Nonalcoholic Fatty Liver Disease Through Remodeling the Gut Microbiota and Modulating the Serum Metabolism 
- Alpha-lipoic acid activates AMPK to protect against oxidative stress and apoptosis in rats with diabetic peripheral neuropathy 
- Hypoglycemic and hypolipidemic effects of Epigynum auritum in high fat diet and streptozotocin-induced diabetic rats 
- Physicochemical Characteristics and Antidiabetic Properties of the Polysaccharides from Pseudostellaria heterophylla 
- The self-nanoemulsifying drug delivery system of Petiveria alliacea extract reduced the homeostatic model assessment-insulin resistance value, interleukin-6, and tumor necrosis factor-α level in diabetic rat models 
- Serum untargeted metabolomics analysis of the mechanisms of evodiamine on type 2 diabetes mellitus model rats 
- Emodin Alleviates High-Glucose-Induced Pancreatic β-Cell Pyroptosis by Inhibiting NLRP3/GSDMD Signaling 
- Circ_005077 accelerates myocardial lipotoxicity induced by high-fat diet via CyPA/p47PHOX mediated ferroptosis 
- Grape Seed Proanthocyanidins Protect Pancreatic β Cells Against Ferroptosis via the Nrf2 Pathway in Type 2 Diabetes 
- Didymin protects pancreatic beta cells by enhancing mitochondrial function in high-fat diet-induced impaired glucose tolerance 
- Jianpi Shengqing Huazhuo Formula improves abnormal glucose and lipid metabolism in obesity by regulating mitochondrial biogenesis 
- Vitamin D3 alleviates lung fibrosis of type 2 diabetic rats via SIRT3 mediated suppression of pyroptosis 
- Jiang-Tang-San-Huang pill alleviates type 2 diabetes mellitus through modulating the gut microbiota and bile acids metabolism 
- Controlled Interfacial Polymer Self-Assembly Coordinates Ultrahigh Drug Loading and Zero-Order Release in Particles Prepared under Continuous Flow 
Reconfiguration of Gut Microbiota and Reprogramming of Liver Metabolism with Phycobiliproteins Bioactive Peptides to Rehabilitate Obese Rats 
Pi-Dan-Jian-Qing Decoction Ameliorates Type 2 Diabetes Mellitus Through Regulating the Gut Microbiota and Serum Metabolism 
Fucoidan ameliorates glucose metabolism by the improvement of intestinal barrier and inflammatory damage in type 2 diabetic rats 
San-Huang-Yi-Shen Capsule Ameliorates Diabetic Kidney Disease through Inducing PINK1/Parkin-Mediated Mitophagy and Inhibiting the Activation of NLRP3 Signaling Pathway 
Structural modulation of gut microbiota during alleviation of non-alcoholic fatty liver disease with Gynostemma pentaphyllum in rats 
Da-Chai-Hu Decoction Ameliorates High Fat Diet-Induced Nonalcoholic Fatty Liver Disease Through Remodeling the Gut Microbiota and Modulating the Serum Metabolism 
Alpha-lipoic acid activates AMPK to protect against oxidative stress and apoptosis in rats with diabetic peripheral neuropathy 
Hypoglycemic and hypolipidemic effects of Epigynum auritum in high fat diet and streptozotocin-induced diabetic rats 
Physicochemical Characteristics and Antidiabetic Properties of the Polysaccharides from Pseudostellaria heterophylla 
The self-nanoemulsifying drug delivery system of Petiveria alliacea extract reduced the homeostatic model assessment-insulin resistance value, interleukin-6, and tumor necrosis factor-α level in diabetic rat models 
Serum untargeted metabolomics analysis of the mechanisms of evodiamine on type 2 diabetes mellitus model rats 
Emodin Alleviates High-Glucose-Induced Pancreatic β-Cell Pyroptosis by Inhibiting NLRP3/GSDMD Signaling 
Circ_005077 accelerates myocardial lipotoxicity induced by high-fat diet via CyPA/p47PHOX mediated ferroptosis 
Grape Seed Proanthocyanidins Protect Pancreatic β Cells Against Ferroptosis via the Nrf2 Pathway in Type 2 Diabetes 
Didymin protects pancreatic beta cells by enhancing mitochondrial function in high-fat diet-induced impaired glucose tolerance 
Jianpi Shengqing Huazhuo Formula improves abnormal glucose and lipid metabolism in obesity by regulating mitochondrial biogenesis 
Vitamin D3 alleviates lung fibrosis of type 2 diabetic rats via SIRT3 mediated suppression of pyroptosis 
Jiang-Tang-San-Huang pill alleviates type 2 diabetes mellitus through modulating the gut microbiota and bile acids metabolism 
Controlled Interfacial Polymer Self-Assembly Coordinates Ultrahigh Drug Loading and Zero-Order Release in Particles Prepared under Continuous Flow 
评价
目前还没有评价