EK2236

Mouse IFN-β ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥2,000.00 – ¥10,800.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：IFNB1, IFN-β, IFNB
  • 种属：小鼠 (Mouse)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：20 μL
  • 灵敏度：0.83 pg/mL
  • 检测范围：15.63 pg/mL - 1,000 pg/mL
  • 回收率：91% - 109%

在售SKU:70-EK2236-48, 70-EK2236-96

描述

商品名

Mouse IFN-β ELISA Kit (小鼠IFN-β ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：3.1% - 4.7%；板间变异系数：3.5% - 4.3%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

20 μL
灵敏度

0.83 pg/mL
检测范围

15.63 pg/mL - 1,000 pg/mL
回收率

91% - 109%
平均回收率

1.03
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗Mouse IFN-β单克隆抗体的96孔聚苯乙烯酶标板
  • Mouse IFN-β冻干标准品
  • Mouse IFN-β检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IFN-β抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、检测样本和辣根过氧化物酶标记的检测抗体，经过孵育，样本中存在的IFN-β与固相抗体和检测抗体结合。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IFN-β的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IFN-β ELISA Kit (小鼠IFN-β ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 IFNB1, IFN-β, IFNB 靶点信息

IFNB1 分子靶点信息概述

  • 分子名：IFNB1, interferon beta 1
  • 基因家族：Interferons
  • 别名：IFB; IFF
  • 曾用名：IFNB
  • 全称：interferon, beta 1, fibroblast

IFNB1 分子靶点综述

干扰素(IFNs)是宿主细胞在响应病原体(如病毒、细菌、寄生虫或者肿瘤细胞)时合成和释放的一组信号蛋白。在通常情况下，病毒感染的细胞会释放干扰素，使周围的细胞提高其抗病毒防御能力。基于受体类型，人类干扰素可分为3大类型：I型干扰素，包括IFN-α、IFN-β、IFN-ε、IFN-κ和IFN-ω，II型干扰素(在人类中称为IFN-γ)和III型干扰素。IFN-β通过信号通路STAT1和STAT2上调和下调多种基因，其中，大部分基因参与抗病毒免疫反应。IFN-β在诱导广泛的非特异性抗病毒感染中起重要作用，它还影响细胞增殖并调节免疫应答。

小鼠 Mouse Ifnb1 分子靶点信息

小鼠 Mouse Ifnb1 靶点分子功能（预测）

Enables cytokine activity and type I interferon receptor binding activity. Involved in B cell proliferation; cellular response to virus; and type I interferon signaling pathway. Acts upstream of or within several processes, including cellular response to organic cyclic compound; defense response to other organism; and negative regulation of osteoclast differentiation. Located in extracellular space. Is expressed in embryo and liver. Human ortholog(s) of this gene implicated in COVID-19. Orthologous to human IFNB1 (interferon beta 1).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. A Network Pharmacology-Based Investigation to the Pharmacodynamic Material Basis and Mechanisms of the Anti-Inflammatory and Anti-Viral Effect of Isatis indigotica 

  2. Tanreqing Inhibits LPS-Induced Acute Lung Injury In Vivo and In Vitro Through Downregulating STING Signaling Pathway 

  3. Anti-influenza A virus mechanism of three representative compounds from Flos Trollii via TLRs signaling pathways 

  4. Simultaneous innate immunity activation and immunosuppression improvement by biodegradable nanoplatform for boosting antitumor chemo-immunotherapy 

  5. Irradiation combined with PD-L1−/− and autophagy inhibition enhances the antitumor effect of lung cancer via cGAS-STING-mediated T cell activation 

  6. Multifunctional biomimetic nanoplatform based on photodynamic therapy and DNA repair intervention for the synergistic treatment of breast cancer 

  7. Toxoplasma gondii ROP18I inhibits host innate immunity through cGAS-STING signaling 

  8. In-situ self-assembled vaccine constructed with dual switchable nanotransformer for tumor immunotherapy 

  9. PreS/2-21-Guided siRNA Nanoparticles Target to Inhibit Hepatitis B Virus Infection and Replication. 

  10. Herpesvirus-Mimicking DNAzyme-Loaded Nanoparticles as a Mitochondrial DNA Stress Inducer to Activate Innate Immunity for Tumor Therapy 

  11. Anlotinib enhances the antitumor immunity of radiotherapy by activating cGAS/STING in non-small cell lung cancer 

  12. NLRP3 protects mice from radiation-induced colon and skin damage via attenuating cGAS-STING signaling 

  13. Integrated manganese (III)-doped nanosystem for optimizing photothermal ablation: Amplifying hyperthermia-induced STING pathway and enhancing antitumor immunity 

  14. Efficient Delivery of Chlorin e6 by Polyglycerol-Coated Iron Oxide Nanoparticles with Conjugated Doxorubicin for Enhanced Photodynamic Therapy of Melanoma 

  15. SHP2-Mediated Inhibition of DNA Repair Contributes to cGAS–STING Activation and Chemotherapeutic Sensitivity in Colon Cancer 

  16. Harnessing chlorin e6 loaded by functionalized iron oxide nanoparticles linked with glucose for target photodynamic therapy and improving of the immunogenicity of lung cancer 

  17. Activating cGAS-STING pathway with ROS-responsive nanoparticles delivering a hybrid prodrug for enhanced chemo-immunotherapy 

  18. Oroxylin A regulates cGAS DNA hypermethylation induced by methionine metabolism to promote HSC senescence 

  19. Allosteric inhibition reveals SHP2-mediated tumor immunosuppression in colon cancer by single-cell transcriptomics 

  20. Hybrid nanoparticle–mediated simultaneous ROS scavenging and STING activation improve the antitumor immunity of in situ vaccines 

  21. Targeted Delivery of STING Agonist via Albumin Nanoreactor Boosts Immunotherapeutic Efficacy against Aggressive Cancers 

  22. 2D Differential Metallic Immunopotentiators Drive High Diversity and Capability of Antigen-specific Immunity Against Tumor 

  23. She-Chuang-Si-Wu-Tang Alleviates Inflammation and Itching Symptoms in a Psoriasis Mouse Model by Regulating the Th17/IL-17 Axis via the STAT3/MAPK Pathways 

  24. Loss of Nrf2 aggravates ionizing radiation-induced intestinal injury by inhibiting the cGAS/STING pathway via Pirin 

  25. Loss of Annexin A1 in macrophages restrains efferocytosis and remodels immune microenvironment in pancreatic cancer by activating the cGAS/STING pathway 

  26. A polymeric nanoplatform enhances the cGAS-STING pathway in macrophages to potentiate phagocytosis for cancer immunotherapy 

  27. Neopterin mediates sleep deprivation-induced microglial activation resulting in neuronal damage by affecting YY1/HDAC1/TOP1/IL-6 signaling 

  28. Chronological Management of Adjuvant Effect for Optimized mRNA Vaccine Inspired by Natural Virus Infection 

  29. Self-modifying NanoEnhancers facilitating lysosomal escape for cGAS-STING cascading activation in tumor immunotherapy 

  30. Engineered low-pathogenic Helicobacter pylori as orally tumor immunomodulators for the stimulation of systemic immune response 

  31. Total glucosides of paeony alleviates cGAS-STING-mediated diseases by blocking the STING-IRF3 interaction 

  32. Peroxidase-Like Nanozyme Activates the cGAS-STING Pathway via ROS-Induced mtDNA Release for Cancer Immunotherapy 

  33. Ginsenoside Rb1 mitigates acute catecholamine surge-induced myocardial injuries in part by suppressing STING-mediated macrophage activation 

  34. Pseudorabies virus usurps non-muscle myosin heavy chain IIA to dampen viral DNA recognition by cGAS for antagonism of host antiviral innate immunity 

  35. Viperin inhibits interferon-γ production to promote Mycobacterium tuberculosis survival by disrupting TBK1-IKKε-IRF3-axis and JAK-STAT signaling 

  36. Dual deficiency of melatonin and dihydrotestosterone promotes stromal cell damage and mediates prostatitis via the cGAS-STING pathway in sleep-deprived mice 

  37. Sequential administration of delta-tocotrienol ameliorates radiation-induced myelosuppression in mice and non-human primates through inducing G-CSF production 

  38. Luteolin-7-O-glucoside promotes macrophage release of IFN-β by maintaining mitochondrial function and corrects the disorder of glucose metabolism during RSV infection 

  39. Echinococcus granulosus cyst fluid inhibits the type I interferon response by promoting ROS in macrophages 

  40. Manganese@Albumin Nanocomplex and Its Assembled Nanowire Activate TLR4-Dependent Signaling Cascades of Macrophages 

  41. Nuclear-Targeted Nanostrategy Regulates Spatiotemporal Communication for Dual Antitumor Immunity 

  42. Ginsenoside Rg3 enriches SCFA-producing commensal bacteria to confer protection against enteric viral infection via the cGAS-STING-type I IFN axis 

  43. High-Intensity Interval Training Ameliorates High-Fat Diet-Induced Metabolic Disorders via the Cyclic GMP-AMP Synthase-Stimulator of Interferon Gene Signaling Pathway 

  44. Manganese ion gradient liposomes modified by disulfide bond enhance cellular uptake and tumor immunity 

  45. Record-High Ultrasound-Sensitive NO Nanogenerators for Cascade Tumor Pyroptosis and Immunotherapy 

  46. Gut microbial GABAergic signaling improves stress-associated innate immunity to respiratory viral infection 

  47. Regulating T-cell metabolic reprogramming and blocking PD-1 co-promote personalized postoperative autologous nanovaccines 

  48. Proteotranscriptomic analyses reveal distinct interferon-beta signaling pathways and therapeutic targets in choroidal neovascularization 

  49. Chronic exposure to low-level lipopolysaccharide dampens influenza-mediated inflammatory response via A20 and PPAR network 

  50. A polymeric nanoplatform enhances the cGAS-STING pathway in macrophages to potentiate phagocytosis for cancer immunotherapy 

  51. A polymeric nanoplatform enhances the cGAS-STING pathway in macrophages to potentiate phagocytosis for cancer immunotherapy 

  52. 细胞培养上清

    Multifunctional biomimetic nanoplatform based on photodynamic therapy and DNA repair intervention for the synergistic treatment of breast cancer 

  53. RAW 264.7 细胞培养上清

    A Network Pharmacology-Based Investigation to the Pharmacodynamic Material Basis and Mechanisms of the Anti-Inflammatory and Anti-Viral Effect of Isatis Indigotica 

  54. 细胞培养上清

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