EK2127S

Mouse BDNF Standard (小鼠脑源性神经营养因子 (BDNF) 标准品)

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¥180.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:BDNF, neurotrophin
  • 种属:小鼠 (Mouse)
  • 试剂盒:EK2127
  • 保存:短期4℃保存,长期-20℃保存
  • 运输条件:4℃蓝冰运输

在售SKU:70-EK2127S


描述

本产品只包含标准品试剂,如需购买试剂盒请点击下图

商品名

Mouse BDNF Standard (小鼠脑源性神经营养因子 (BDNF) 标准品)

组分

小鼠BDNF冻干标准品

检测方法

双抗夹心法

样本类型

血清,血浆,细胞培养上清及其他生物学样本

板式

保存

短期4℃,长期-20℃保存

运输条件

4℃蓝冰运输

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠BDNF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的BDNF与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中BDNF的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse BDNF ELISA Kit (小鼠脑源性神经营养因子 (BDNF) ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 BDNF, neurotrophin 靶点信息

BDNF 分子靶点信息概述

  • 分子名:BDNF, brain derived neurotrophic factor
  • 基因家族:Neurotrophins
  • 全称:neurotrophin

BDNF 分子靶点综述

脑源性神经营养因子(BDNF)是生长因子神经营养素家族中的一员,与神经生长因子相关。神经营养因子存在于脑及外周。BDNF在内质网中加工合成,由致密核心囊泡分泌。它在大脑中的海马、皮层和基底前脑区域活跃,同样也在视网膜、运动神经元、肾脏、唾液和前列腺中表达。BDNF作用于中枢神经系统和外周神经系统的某些神经元,帮助现存神经元的存活、促进新生神经元和突触的生长和分化。BDNF的改变可能在精神分裂症的发病中起作用,与抑郁、湿疹、癫痫、阿尔茨海默病、肥胖、衰老、先天性中枢性低通气综合征和化疗后认知功能障碍有关。

小鼠 Mouse Bdnf 分子靶点信息

小鼠 Mouse Bdnf 靶点分子功能(预测)

Predicted to enable growth factor activity; nerve growth factor receptor binding activity; and neurotrophin TRKB receptor binding activity. Involved in negative regulation of apoptotic signaling pathway; positive regulation of neuron projection development; and retrograde trans-synaptic signaling by neuropeptide, modulating synaptic transmission. Acts upstream of or within several processes, including generation of neurons; modulation of chemical synaptic transmission; and sensory organ development. Located in several cellular components, including dendrite; perinuclear region of cytoplasm; and secretory granule. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in several diseases, including cognitive disorder (multiple); congenital central hypoventilation syndrome; neurodegenerative disease (multiple); obstructive sleep apnea; and status epilepticus. Orthologous to human BDNF (brain derived neurotrophic factor).

操作步骤

ELISA操作常见问题

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引用文献


  1. Melatonin enhances autologous adipose-derived stem cells to improve mouse ovarian function in relation to the SIRT6/NF-κB pathway 

  2. Tenacissoside H promotes neurological recovery of cerebral ischaemia/reperfusion injury in mice by modulating inflammation and oxidative stress via TrkB pathway 

  3. Duloxetine-Induced Neural Cell Death and Promoted Neurite Outgrowth in N2a Cells 

  4. Depletion of placental brain-derived neurotrophic factor (BDNF) is attributed to premature ovarian insufficiency (POI) in mice offspring 

  5. BDNF promotes mouse follicular development and reverses ovarian aging by promoting cell proliferation 

  6. Arctiin alleviates functional constipation by enhancing intestinal motility in mice 

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