Human sP-Selectin ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:SELP, CD62P, GRMP
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:20 μL
- 灵敏度:1.11 pg/mL
- 检测范围:78.13 pg/mL - 5000 pg/mL
- 回收率:88% - 106%
在售SKU:70-EK186-48, 70-EK186-96
描述
商品名 |
Human sP-Selectin ELISA Kit (人可溶性P选择素 ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:5.3% - 7.8%;板间变异系数:5.4% - 9.0% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
20 μL |
灵敏度 |
1.11 pg/mL |
检测范围 |
78.13 pg/mL - 5000 pg/mL |
回收率 |
88% - 106% |
平均回收率 |
0.96 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人sP-Selectin抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的sP-Selectin与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中sP-Selectin的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
SELP 分子靶点信息概述
- 分子名:SELP, selectin P
- 基因家族:CD molecules; Selectins; Sushi domain containing; C-type lectin domain containing
- 别名:CD62; PSEL; PADGEM; GMP140; CD62P
- 曾用名:GRMP
- 全称:antigen CD62; granule membrane protein 140kDa; selectin P (granule membrane protein 140kD, antigen CD62); selectin P (granule membrane protein 140kDa, antigen CD62)
SELP 分子靶点综述
CD62P 是一种 140kDa 跨膜糖蛋白,也称为 P-Selectin。P-Selectin被储存在血小板的 α 颗粒和内皮细胞的 Weibel-Palade 小体中,并在激活后迅速转运到质膜。在炎症反应中,P-Selectin 被认为介导中性粒细胞和单核细胞与内皮的初始粘附相互作用;在止血过程中,活化的血小板与中性粒细胞和单核细胞相互作用。
人 Human SELP 分子靶点信息
- 分子名:SELP, selectin P
- 别称:
- CD62
- CD62 antigen-like family member P
- CD62P
- FLJ45155
- GMP-140
- GMP140
- granule membrane protein 140
- granule membrane protein 140kDa
- granulocyte membrane protein
- GRMP
- LECAM3
- leukocyte-endothelial cell adhesion molecule 3
- P-selectin
- PADGEM
- platelet activation dependent granule-external membrane protein
- platelet alpha-granule membrane protein
- PSEL
- selectin P (granule membrane protein 140kDa, antigen CD62)
- 基因序列:NCBI_Gene: 6403
- 蛋白序列:UniProtKB: P16109
人 Human SELP靶点分子功能(预测)
Enables several functions, including fucose binding activity; heparin binding activity; and lipopolysaccharide binding activity. Involved in several processes, including calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules; calcium-mediated signaling using intracellular calcium source; and leukocyte tethering or rolling. Located in external side of plasma membrane; extracellular space; and platelet alpha granule membrane. Is integral component of plasma membrane. Implicated in atopic dermatitis and myocardial infarction. Biomarker of several diseases, including Kawasaki disease; autoimmune disease (multiple); coronary artery disease (multiple); obesity; and type 2 diabetes mellitus.
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
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- ELISA通关必备丨如何选择试剂盒 
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ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- miR-374b-5p is increased in deep vein thrombosis and negatively targets IL-10 
- Thymic stromal lymphopoietin induces platelet mitophagy and promotes thrombosis in Kawasaki disease 
- Salvianolic acid B inhibits platelets-mediated inflammatory response in vascular endothelial cells 
- A metal-organic-framework incorporated vascular graft for sustained nitric oxide generation and long-term vascular patency 
- Direct thrombin inhibitor-bivalirudin improved the hemocompatibility of electrospun polycaprolactone vascular grafts 
- LncRNA MALAT1 suppresses monocyte-endothelial cell interactions by targeting miR-30b-5p and enhancing ATG5-mediated autophagy 
miR-374b-5p is increased in deep vein thrombosis and negatively targets IL-10 
Thymic stromal lymphopoietin induces platelet mitophagy and promotes thrombosis in Kawasaki disease 
Salvianolic acid B inhibits platelets-mediated inflammatory response in vascular endothelial cells 
A metal-organic-framework incorporated vascular graft for sustained nitric oxide generation and long-term vascular patency 
Direct thrombin inhibitor-bivalirudin improved the hemocompatibility of electrospun polycaprolactone vascular grafts 
LncRNA MALAT1 suppresses monocyte-endothelial cell interactions by targeting miR-30b-5p and enhancing ATG5-mediated autophagy 
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