EK1M03

Human MMP-3 ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:MMP3, MMP-3, STMY
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:100 μL
  • 灵敏度:0.30 pg/mL
  • 检测范围:31.25 pg/mL - 2000 pg/mL
  • 回收率:95% - 105%

在售SKU:70-EK1M03-48, 70-EK1M03-96


描述

商品名

Human MMP-3 ELISA Kit (人基质金属蛋白酶3 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:4.0% - 4.9%;板间变异系数:4.2% - 9.0%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

100 μL

灵敏度

0.30 pg/mL

检测范围

31.25 pg/mL - 2000 pg/mL

回收率

95% - 105%

平均回收率

1.01

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗MMP-3单克隆抗体的96孔聚苯乙烯酶标板
  • 人MMP-3冻干标准品
  • MMP-3检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人MMP-3抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的MMP-3与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中MMP-3的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长5632 nm)测定吸光度值。

Human MMP-3 ELISA Kit (人基质金属蛋白酶3 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 MMP3, MMP-3, STMY 靶点信息

MMP3 分子靶点信息概述

  • 分子名:MMP3, matrix metallopeptidase 3
  • 基因家族:M10 matrix metallopeptidases
  • 曾用名:STMY1; STMY
  • 全称:matrix metalloproteinase 3 (stromelysin 1, progelatinase); stromelysin 1

MMP3 分子靶点综述

基质金属蛋白酶3(MMP-3),又名基质分解素-1,在人类中由MMP3基因编码。MMP3的表达主要在转录水平上调控,转录水平上的基因启动子对多种刺激物包括生长因子、细胞因子、促癌剂和原癌基因产物作出应答。MMP-3酶降解II、III、IV、IX和X型胶原蛋白、蛋白聚糖、纤维连接蛋白、层粘连蛋白和弹性蛋白。另外,MMP-3还可激活其它MMPs如MMP-1、MMP-7和MMP-9,因此MMP-3在结缔组织重塑中起关键作用。该酶参与伤口修复、动脉粥样硬化进展和肿瘤启动。MMP-3野生型和基因敲除的小鼠研究表明,MMP-3增加了外伤性损伤后的血脑屏障通透性。细胞核MMP-3可作为转录因子和蛋白酶发挥作用。

人 Human MMP3 分子靶点信息

  • 分子名:MMP3, matrix metallopeptidase 3
  • 别称:
    • CHDS6
    • matrix metallopeptidase 3 (stromelysin 1, progelatinase)
    • matrix metalloproteinase 3 (stromelysin 1, progelatinase)
    • matrix metalloproteinase-3
    • MGC126102
    • MGC126103
    • MGC126104
    • MMP-3
    • proteoglycanase
    • SL-1
    • STMY
    • STMY1
    • STR1
    • stromelysin-1
    • transin-1
  • 基因序列:NCBI_Gene: 4314
  • 蛋白序列:UniProtKB: P08254

人 Human MMP3靶点分子功能(预测)

Enables endopeptidase activity and metallopeptidase activity. Involved in several processes, including cellular response to UV-A; negative regulation of hydrogen peroxide metabolic process; and positive regulation of protein-containing complex assembly. Predicted to be located in cytosol and mitochondrion. Implicated in several diseases, including artery disease (multiple); carcinoma (multiple); end stage renal disease; osteoarthritis; and von Hippel-Lindau disease. Biomarker of several diseases, including Takayasu's arteritis; arthritis (multiple); autoimmune disease (multiple); conjunctivochalasis; and lung disease (multiple).

操作步骤

ELISA操作常见问题

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Role of ROR2 in promoting gastric cancer metastasis by enhancing c-JUN-mediated MMP3 transcription 

  2. Elevated microRNA‑145‑5p increases matrix metalloproteinase‑9 by activating the nuclear factor‑κB pathway in rheumatoid arthritis 

  3. Follistatin-like protein 1 induction of matrix metalloproteinase 1, 3 and 13 gene expression in rheumatoid arthritis synoviocytes requires MAPK, JAK/STAT3 and NF-κB pathways 

  4. Comparison of the therapeutic effects of human umbilical cord blood-derived mesenchymal stem cells and adipose-derived stem cells on erectile dysfunction in a rat model of bilateral cavernous nerve injury. 

  5. Aconiti Lateralis Radix Praeparata lipid-soluble alkaloids alleviates IL-1β-induced inflammation of human fibroblast-like synoviocytes in rheumatoid arthritis by inhibiting NF-κB and MAPKs signaling pathways and inducing apoptosis 

  6. Structural characterization and anti-photoaging activity of a polysaccharide from Sargassum fusiforme 

  7. The isolation, structure characterizations and anti-photoaging activities of sulfated polysaccharides isolated from Sargassum fusiforme 

  8. The Potential of Bioactive Fish Collagen Oligopeptides against Hydrogen Peroxide-Induced NIH/3T3 and HUVEC Damage: The Involvement of the Mitochondria 

  9. Beneficial Effects of Small-Molecule Oligopeptides Isolated from Panax Ginseng C. A. Meyer on Cellular Fates in Oxidative Stress-Induced Damaged Human Umbilical Vein Endothelial Cells and PC-12 

  10. Sulfasalazine promotes ferroptosis through AKT-ERK1/2 and P53-SLC7A11 in rheumatoid arthritis 

  11. Knockdown of Galectin-9 alleviates rheumatoid arthritis through suppressing TNF-α-induced activation of fibroblast-like synoviocytes 

  12. Maslinic acid attenuates UVB-induced oxidative damage in HFF-1 cells 

  13. The effects of alginate lyase treatment on structural characteristics and anti-photoaging activity of alginate from Sargassum fusiforme 

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