EK3127

Rat BDNF ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:BDNF, neurotrophin
  • 种属:大鼠 (Rat)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:100 μL(稀释液)
  • 灵敏度:0.54 pg/mL
  • 检测范围:3.91 pg/mL - 250 pg/mL
  • 回收率:84% - 118%

在售SKU:70-EK3127-48, 70-EK3127-96


描述

商品名

Rat BDNF ELISA Kit (大鼠脑源性神经营养因子 (BDNF) ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.7% - 4.7%;板间变异系数:3.9%-4.9%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

100 μL(稀释液)

灵敏度

0.54 pg/mL

检测范围

3.91 pg/mL - 250 pg/mL

回收率

84% - 118%

平均回收率

1.05

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗BDNF单克隆抗体的96孔聚苯乙烯酶标板
  • 大鼠BDNF冻干标准品
  • BDNF检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠BDNF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的BDNF与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中BDNF的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Rat BDNF ELISA Kit (大鼠脑源性神经营养因子 (BDNF) ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述大鼠 BDNF, neurotrophin 靶点信息

BDNF 分子靶点信息概述

  • 分子名:BDNF, brain derived neurotrophic factor
  • 基因家族:Neurotrophins
  • 全称:neurotrophin

BDNF 分子靶点综述

脑源性神经营养因子(BDNF)是生长因子神经营养素家族中的一员,与神经生长因子相关。神经营养因子存在于脑及外周。BDNF在内质网中加工合成,由致密核心囊泡分泌。它在大脑中的海马、皮层和基底前脑区域活跃,同样也在视网膜、运动神经元、肾脏、唾液和前列腺中表达。BDNF作用于中枢神经系统和外周神经系统的某些神经元,帮助现存神经元的存活、促进新生神经元和突触的生长和分化。BDNF的改变可能在精神分裂症的发病中起作用,与抑郁、湿疹、癫痫、阿尔茨海默病、肥胖、衰老、先天性中枢性低通气综合征和化疗后认知功能障碍有关。

大鼠 Rat Bdnf 分子靶点信息

大鼠 Rat Bdnf 靶点分子功能(预测)

Enables neurotrophin TRKB receptor binding activity. Involved in several processes, including behavioral response to cocaine; cellular response to norepinephrine stimulus; and regulation of neuronal synaptic plasticity. Acts upstream of or within positive regulation of DNA-binding transcription factor activity; positive regulation of glucocorticoid receptor signaling pathway; and positive regulation of peptidyl-serine phosphorylation. Located in several cellular components, including mitochondrial crista; perikaryon; and synaptic vesicle. Used to study Alzheimer's disease; cystitis; eye disease (multiple); neuropathy (multiple); and status epilepticus. Biomarker of several diseases, including alcohol use disorder; attention deficit hyperactivity disorder; hypertensive encephalopathy; middle cerebral artery infarction; and sciatic neuropathy. Human ortholog(s) of this gene implicated in several diseases, including cognitive disorder (multiple); congenital central hypoventilation syndrome; neurodegenerative disease (multiple); obstructive sleep apnea; and status epilepticus. Orthologous to human BDNF (brain derived neurotrophic factor).

操作步骤

ELISA操作常见问题

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引用文献


  1. In vitro study of decellularized rat tissues for nerve regeneration. 

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  3. Safety evaluation and protective effects of ethanolic extract from maca (Lepidium meyenii Walp.) against corticosterone and H2O2 induced neurotoxicity 

  4. Integrated analysis of phytochemical composition, pharmacokinetics, and network pharmacology to probe distinctions between the stems of Cistanche deserticola and C. tubulosa based on antidepressant activity 

  5. Intratympanic injection of MSC-derived small extracellular vesicles protects spiral ganglion neurons from degeneration 

  6. Anti-neuroinflammatory effect of hydroxytyrosol: a potential strategy for anti-depressant development 

  7. 细胞培养上清

    Nanofat functionalized injectable super-lubricating microfluidic microspheres for treatment of osteoarthritis 

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