EK2208

Mouse TRANCE/TNFSF11/RANKL ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:TNFSF11, RANKL, CD254
  • 种属:小鼠 (Mouse)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:20 μL
  • 灵敏度:4.23 pg/mL
  • 检测范围:31.25 pg/mL - 2000 pg/mL
  • 回收率:83% - 116%

在售SKU:70-EK2208-48, 70-EK2208-96


描述

商品名

Mouse TRANCE/TNFSF11/RANKL ELISA Kit (小鼠肿瘤坏死因子相关激活诱导因子/肿瘤坏死因子配体超家族成员11/核因子κB受体活化因子配基 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:6.0%-8.9%;板间变异系数:5.8% - 8.6%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

20 μL

灵敏度

4.23 pg/mL

检测范围

31.25 pg/mL - 2000 pg/mL

回收率

83% - 116%

平均回收率

1

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗TRANCE单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠TRANCE冻干标准品
  • TRANCE检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠 TRANCE 抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的 TRANCE 与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物 TMB,避光显色。颜色反应的深浅与样本中 TRANCE 的浓度成正比。加入终止液终止反应,在 450 nm 波长(参考波长 570 - 630 nm)测定吸光度值。

Mouse TRANCE/TNFSF11/RANKL ELISA Kit (小鼠肿瘤坏死因子相关激活诱导因子/肿瘤坏死因子配体超家族成员11/核因子κB受体活化因子配基 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 TNFSF11, RANKL, CD254 靶点信息

TNFSF11 分子靶点信息概述

  • 分子名:TNFSF11, TNF superfamily member 11
  • 基因家族:CD molecules; Tumor necrosis factor superfamily
  • 别名:TRANCE; RANKL; OPGL; ODF; CD254
  • 全称:tumor necrosis factor (ligand) superfamily, member 11

TNFSF11 分子靶点综述

肿瘤坏死因子相关激活诱导因子(TRANCE),又名肿瘤坏死因子配体超家族成员11(TNFSF11)、核因子κB受体活化因子配基(RANKL),是TNF超家族成员。成骨细胞、CD4+和CD8+T细胞、巨核细胞、支气管和肠道上皮细胞等都可表达TRANCE。TRANCE在免疫应答和骨形态发生中起核心作用。在骨形成和重塑过程中,成骨细胞表达的TRANCE与M-CSF一起诱导破骨细胞形成和活化,导致骨吸收,进而导致随后的骨沉积和重塑。TRANCE同样在病理性骨质疏松中起作用。活化的淋巴细胞和成骨细胞不恰当表达TRANCE就会导致关节和骨头的过度吸收与沉积。过量的TRANCE与许多退行性骨疾病,如类风湿性关节炎、银屑病性关节炎有关。

小鼠 Mouse Tnfsf11 分子靶点信息

  • 分子名:Tnfsf11, tumor necrosis factor (ligand) superfamily, member 11
  • 别称:
    • Ly109l
    • ODF
    • OPGL
    • osteoclast differentiation factor
    • RANKL
    • TNF-related activation-induced cytokine
    • Trance
  • 基因序列:NCBI_Gene: 21943
  • 蛋白序列:

小鼠 Mouse Tnfsf11 靶点分子功能(预测)

Enables identical protein binding activity and tumor necrosis factor receptor superfamily binding activity. Involved in several processes, including intracellular signal transduction; positive regulation of secretion by cell; and tumor necrosis factor-mediated signaling pathway. Acts upstream of or within several processes, including animal organ development; cellular response to cytokine stimulus; and positive regulation of intracellular signal transduction. Predicted to be located in cytoplasm; extracellular space; and plasma membrane. Predicted to be integral component of membrane. Is expressed in genitourinary system; hemolymphoid system; hindlimb long bone; and lymphatic system. Used to study autosomal recessive osteopetrosis 2 and osteoporosis. Human ortholog(s) of this gene implicated in autosomal recessive osteopetrosis 2 and osteoporosis. Orthologous to human TNFSF11 (TNF superfamily member 11).

操作步骤

ELISA操作常见问题

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引用文献


  1. Characterizations and the Mechanism Underlying Osteogenic Activity of Peptides from Enzymatic Hydrolysates of Stichopus japonicus 

  2. ZhiJingSan Inhibits Osteoclastogenesis via Regulating RANKL/NF-κB Signaling Pathway and Ameliorates Bone Erosion in Collagen-Induced Mouse Arthritis 

  3. Inhibitory effects of Atlantic cod (Gadus morhua) peptides on RANKL-induced osteoclastogenesis in vitro and osteoporosis in ovariectomized mice 

  4. Supranutritional selenium suppresses ROS-induced generation of RANKL-expressing osteoclastogenic CD4+ T cells and ameliorates rheumatoid arthritis 

  5. Absorption and transport properties of a codfish-derived peptide and its protective effect on bone loss in ovariectomized mice 

  6. Myosin heavy chain-derived peptide of Gadus morhua promotes proliferation and differentiation in osteoblasts and bone formation and maintains bone homeostasis in ovariectomized mice 

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