Mouse Total MMP-3 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥2,000.00 – ¥3,400.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:MMP3, MMP-3, STMY
- 种属:小鼠 (Mouse)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:100 μL(稀释液)
- 灵敏度:15.99 pg/mL
- 检测范围:46.88 pg/mL - 3000 pg/mL
- 回收率:75% - 95%
在售SKU:70-EK2M03-48, 70-EK2M03-96
描述
商品名 |
Mouse Total MMP-3 ELISA Kit (小鼠总基质金属蛋白酶3 (total MMP-3) ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:2.9% - 3.6%;板间变异系数:3.4%-4.8% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
100 μL(稀释液) |
灵敏度 |
15.99 pg/mL |
检测范围 |
46.88 pg/mL - 3000 pg/mL |
回收率 |
75% - 95% |
平均回收率 |
0.88 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠total MMP-3抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的total MMP-3与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中total MMP-3的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
MMP3 分子靶点信息概述
- 分子名:MMP3, matrix metallopeptidase 3
- 基因家族:M10 matrix metallopeptidases
- 曾用名:STMY1; STMY
- 全称:matrix metalloproteinase 3 (stromelysin 1, progelatinase); stromelysin 1
MMP3 分子靶点综述
基质金属蛋白酶3(MMP-3),又名基质分解素-1,在人类中由MMP3基因编码。MMP3的表达主要在转录水平上调控,转录水平上的基因启动子对多种刺激物包括生长因子、细胞因子、促癌剂和原癌基因产物作出应答。MMP-3酶降解II、III、IV、IX和X型胶原蛋白、蛋白聚糖、纤维连接蛋白、层粘连蛋白和弹性蛋白。另外,MMP-3还可激活其它MMPs如MMP-1、MMP-7和MMP-9,因此MMP-3在结缔组织重塑中起关键作用。该酶参与伤口修复、动脉粥样硬化进展和肿瘤启动。MMP-3野生型和基因敲除的小鼠研究表明,MMP-3增加了外伤性损伤后的血脑屏障通透性。细胞核MMP-3可作为转录因子和蛋白酶发挥作用。
小鼠 Mouse Mmp3 分子靶点信息
- 分子名:Mmp3, matrix metallopeptidase 3
- 别称:
- progelatinase
- SLN-1
- SLN1
- Stmy1
- STR-1
- Str1
- stromelysin 1
- stromelysin-1
- 基因序列:NCBI_Gene: 17392
- 蛋白序列:
小鼠 Mouse Mmp3 靶点分子功能(预测)
Predicted to enable metalloendopeptidase activity. Involved in several processes, including negative regulation of protein kinase B signaling; positive regulation of oxidative stress-induced cell death; and protein catabolic process. Acts upstream of or within cellular response to amino acid stimulus and regulation of cell migration. Located in cytosol and mitochondrion. Is expressed in several structures, including axial skeleton thoracic region; genitourinary system; liver; oral region epithelium; and palatal shelf. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); carcinoma (multiple); end stage renal disease; osteoarthritis; and von Hippel-Lindau disease. Orthologous to human MMP3 (matrix metallopeptidase 3).
操作步骤
文章目录[隐藏]
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引用文献
文章目录[隐藏]
- Bioactive Oligopeptides from Ginseng (Panax ginseng Meyer) Suppress Oxidative Stress-Induced Senescence in Fibroblasts via NAD+/SIRT1/PGC-1α Signaling Pathway 
- A non-Bactericidal Cathelicidin with Antioxidant Properties Ameliorates UVB-Induced Mouse Skin Photoaging via Intracellular ROS Scavenging and Keap1/Nrf2 Pathway Activation 
- The Potential of Bioactive Fish Collagen Oligopeptides against Hydrogen Peroxide-Induced NIH/3T3 and HUVEC Damage: The Involvement of the Mitochondria 
- Beneficial Effects of Small-Molecule Oligopeptides Isolated from Panax Ginseng C. A. Meyer on Cellular Fates in Oxidative Stress-Induced Damaged Human Umbilical Vein Endothelial Cells and PC-12 
- SIRT1 inhibitors within Qing-Luo-Yin alleviated white adipose tissues-mediated inflammation in antigen-induced arthritis mice 
Bioactive Oligopeptides from Ginseng (Panax ginseng Meyer) Suppress Oxidative Stress-Induced Senescence in Fibroblasts via NAD+/SIRT1/PGC-1α Signaling Pathway 
A non-Bactericidal Cathelicidin with Antioxidant Properties Ameliorates UVB-Induced Mouse Skin Photoaging via Intracellular ROS Scavenging and Keap1/Nrf2 Pathway Activation 
The Potential of Bioactive Fish Collagen Oligopeptides against Hydrogen Peroxide-Induced NIH/3T3 and HUVEC Damage: The Involvement of the Mitochondria 
Beneficial Effects of Small-Molecule Oligopeptides Isolated from Panax Ginseng C. A. Meyer on Cellular Fates in Oxidative Stress-Induced Damaged Human Umbilical Vein Endothelial Cells and PC-12 
SIRT1 inhibitors within Qing-Luo-Yin alleviated white adipose tissues-mediated inflammation in antigen-induced arthritis mice 
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