EK9131S

Mouse/Rat IGF-1 Standard (小鼠/大鼠IGF-1 标准品)

X

¥180.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:IGF1, IGFI
  • 种属:小鼠 (Mouse); 大鼠 (Rat)
  • 试剂盒:EK9131
  • 保存:短期4℃保存,长期-20℃保存
  • 运输条件:4℃蓝冰运输

在售SKU:70-EK9131S


描述

本产品只包含标准品试剂,如需购买试剂盒请点击下图

商品名

Mouse/Rat IGF-1 Standard (小鼠/大鼠IGF-1 标准品)

组分

小鼠/大鼠IGF-1标准品

检测方法

双抗夹心法

样本类型

血清,血浆,细胞培养上清及其他生物学样本

板式

保存

短期4℃,长期-20℃保存

运输条件

4℃蓝冰运输

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠/大鼠IGF-1抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的IGF-1与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IGF-1的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse/Rat IGF-1 ELISA Kit (小鼠/大鼠IGF-1 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述

IGF1 分子靶点信息概述

  • 分子名:IGF1, insulin like growth factor 1
  • 基因家族:Neuropeptides
  • 别名:IGF1A; IGFI; IGF-I; IGF
  • 全称:somatomedin C; insulin-like growth factor 1 (somatomedin C)

IGF1 分子靶点综述

胰岛素样生长因子1(IGF-1),也称为生长调节素C,是一个在分子结构上类似于胰岛素的激素。IGF-1主要由肝脏产生,作为内分泌激素。同样地,靶组织亦能旁分泌/自分泌产生IGF-1。IGF-1是AKT信号转导途径中的一种有效天然激活剂、细胞生长和增殖的刺激子、细胞程序性死亡的有效抑制子。IGF-1最少可结合两种细胞表面受体:IGF-1受体(IGF1R)和胰岛素受体。IGF-1的产生受生长激素刺激,受营养不良、生长激素不敏感、缺少生长激素受体或者生长激素受体(SHP2和STAT5B)下游信号通路发生故障等方面所抑制。IGF-1在儿童生长过程中起重要作用,继而在成人合成代谢中有影响,且与老年化、神经病变、癌症以及中风相关。

小鼠 Mouse Igf1 分子靶点信息

小鼠 Mouse Igf1 靶点分子功能(预测)

Enables insulin receptor binding activity and insulin-like growth factor receptor binding activity. Involved in negative regulation of gene expression; negative regulation of release of cytochrome c from mitochondria; and positive regulation of myelination. Acts upstream of or within several processes, including animal organ development; positive regulation of cell population proliferation; and regulation of signal transduction. Located in exocytic vesicle and extracellular space. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; limb; and sensory organ. Human ortholog(s) of this gene implicated in several diseases, including acne; artery disease (multiple); diabetes mellitus (multiple); myopia; and neurodegenerative disease (multiple). Orthologous to human IGF1 (insulin like growth factor 1).

操作步骤

ELISA操作常见问题

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Dual-Functionalized MSCs that Express CX3CR1 and IL-25 Exhibit Enhanced Therapeutic Effects on Inflammatory Bowel Disease 

  2. PLGA Microspheres of hGH of Preserved Native State Prepared Using a Self-Regulated Process 

  3. Engineered adipose-derived stem cells with IGF-1-modified mRNA ameliorates osteoarthritis development 

  4. Chondroprotective effects of platelet lysate towards monoiodoacetate-induced arthritis by suppression of TNF-α-induced activation of NF-ĸB pathway in chondrocytes 

  5. Chitosan-Gelatin Scaffolds Incorporating Decellularized Platelet-Rich Fibrin Promote Bone Regeneration 

  6. Macrophage polarization plays roles in bone formation instructed by calcium phosphate ceramics 

  7. Actively separated microneedle patch for sustained-release of growth hormone to treat growth hormone deficiency 

  8. IGF-1 and myostatin-mediated co-regulation in skeletal muscle and bone of Daurian ground squirrels (Spermophilus dauricus) during different hibernation stages 

  9. A long-acting FGF21 attenuates metabolic dysfunction-associated steatohepatitis-related fibrosis by modulating NR4A1-mediated Ly6C phenotypic switch in macrophages 

  10. Regeneration of Thyroid Glands in the Spleen Restores Homeostasis in Thyroidectomy Mice 

  11. Cartilage-targeting mRNA-lipid nanoparticles rescue perifocal apoptotic chondrocytes for integrative cartilage repair 

  12. Insulin-like growth factor binding protein 4 loaded electrospun membrane ameliorating tendon injury by promoting retention of IGF-1 

  13. 细胞培养上清 间充质干细胞

    Dual-Functionalized MSCs that Express CX3CR1 and IL-25 Exhibit Enhanced Therapeutic Effects on Inflammatory Bowel Disease 

评价

目前还没有评价

成为第一个“Mouse/Rat IGF-1 Standard (小鼠/大鼠IGF-1 标准品)” 的评价者

您的电子邮箱地址不会被公开。 必填项已用*标注