EK205HS

Mouse IL-5 High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥2,000.00 – ¥3,400.00

  • 分子靶点：IL5, IL-5, TRF
  • 种属：小鼠 (Mouse)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：20 μL
  • 灵敏度：0.14 pg/mL
  • 检测范围：1.56 pg/mL - 100 pg/mL
  • 回收率：104% - 107%

在售SKU:70-EK205HS-48, 70-EK205HS-96

描述

商品名

Mouse IL-5 High Sensitivity ELISA Kit (小鼠白细胞介素5 (IL-5) 高敏 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：4.8% - 5.0%；板间变异系数：3.0% - 5.3%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

20 μL
灵敏度

0.14 pg/mL
检测范围

1.56 pg/mL - 100 pg/mL
回收率

104% - 107%
平均回收率

1.05
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IL-5单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠IL-5冻干标准品
  • IL-5检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 信号增强剂浓缩液
  • 信号增强剂稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-5抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的IL-5与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-5的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IL-5 High Sensitivity ELISA Kit (小鼠白细胞介素5 (IL-5) 高敏 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 IL5, IL-5, TRF 靶点信息

IL5 分子靶点信息概述

  • 分子名：IL5, interleukin 5
  • 基因家族：Interleukins
  • 别名：IL-5; EDF; TRF
  • 全称：interleukin-5; T-cell replacing factor; B cell differentiation factor I; eosinophil differentiation factor; colony-stimulating factor, eosinophil; interleukin 5 (colony-stimulating factor, eosinophil)

IL5 分子靶点综述

白细胞介素5(IL-5)是造血家族Th2细胞因子成员，由115个氨基酸组成。与该细胞因子家族其它成员(IL-3和GM-CSF)不同，该糖蛋白的活化形式是同源二聚体。IL-5由Th2细胞和肥大细胞分泌。它对B细胞以及其它类型的细胞产生的生物学活性表明，IL-5对Th2细胞的活性具有重要的功能性作用。IL-5通过与其受体结合，刺激B细胞生长，提高免疫球蛋白分泌。它也是活化嗜酸性粒细胞的关键因子。IL-5一直以来与多种变态疾病相关，包括变应性鼻炎和哮喘，在循环组织、气管组织中观察到大幅增加的IL-5，而痰嗜酸性粒细胞则减少。

小鼠 Mouse Il5 分子靶点信息

小鼠 Mouse Il5 靶点分子功能（预测）

Enables cytokine activity. Involved in positive regulation of DNA-binding transcription factor activity; positive regulation of immunoglobulin production; and positive regulation of transcription, DNA-templated. Acts upstream of or within positive regulation of peptidyl-tyrosine phosphorylation. Located in extracellular space. Is expressed in colon; thymus; and thymus primordium. Human ortholog(s) of this gene implicated in asthma. Orthologous to human IL5 (interleukin 5).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Small Proline-Rich Protein 3 Regulates IL-33/ILC2 Axis to Promote Allergic Airway Inflammation 

  2. Effects of divalent cations on the physical, conformational and immunological properties of bovine allergen β-lactoglobulin aggregates 

  3. RETRACTED ARTICLE: Down-regulation of microRNA-342-5p or Up-regulation of Wnt3a Inhibits Angiogenesis and Maintains Atherosclerotic Plaque Stability in Atherosclerosis Mice 

  4. LncRNA AK085865 depletion ameliorates asthmatic airway inflammation by modulating macrophage polarization 

  5. HMGB1-induced ILC2s activate dendritic cells by producing IL-9 in asthmatic mouse model 

  6. Mucosal immunity of mannose-modified chitosan microspheres loaded with the nontyepable Haemophilus influenzae outer membrane protein P6 in BALB/c mice 

  7. Louki Zupa decoction attenuates the airway inflammation in acute asthma mice induced by ovalbumin through IL-33/ST2-NF-κB/GSK3β/mTOR signalling pathway 

  8. TMT-based quantitative proteomics reveals suppression of SLC3A2 and ATP1A3 expression contributes to the inhibitory role of acupuncture on airway inflammation in an OVA-induced mouse asthma model 

  9. Icariside II attenuates eosinophils-induced airway inflammation and remodeling via inactivation of NF-κB and STAT3 in an asthma mouse model 

  10. Melatonin modulates airway smooth muscle cell phenotype by targeting the STAT3/Akt/GSK-3β pathway in experimental asthma 

  11. Long non-coding RNA NKILA alleviates airway inflammation in asthmatic mice by promoting M2 macrophage polarization and inhibiting the NF-κB pathway 

  12. Solid lipid nanoparticle delivery of rhynchophylline enhanced the efficiency of allergic asthma treatment via the upregulation of suppressor of cytokine signaling 1 by repressing the p38 signaling pathway 

  13. Rhynchophylline attenuates allergic bronchial asthma by inhibiting transforming growth factor‑β1‑mediated Smad and mitogen‑activated protein kinase signaling transductions in vivo and in vitro 

  14. Branched-chain amino acid transaminase 1 inhibition attenuates childhood asthma in mice by effecting airway remodeling and autophagy 

  15. TMT-Based Quantitative Proteomic Analysis Reveals Downregulation of ITGAL and Syk by the Effects of Cycloastragenol in OVA-Induced Asthmatic Mice 

  16. Isorhynchophylline exerts anti-asthma effects in mice by inhibiting the proliferation of airway smooth muscle cells: The involvement of miR-200a-mediated FOXC1/NF-κB pathway 

  17. Suppression of Hippo Pathway by Food Allergen Exacerbates Intestinal Epithelia Instability and Facilitates Hypersensitivity 

  18. Therapeutic Effect of Renifolin F on Airway Allergy in an Ovalbumin-Induced Asthma Mouse Model In Vivo 

  19. Jia-Wei-Yu-Ping-Feng-San Attenuates Group 2 Innate Lymphoid Cell-Mediated Airway Inflammation in Allergic Asthma 

  20. MiRNA-192-5p attenuates airway remodeling and autophagy in asthma by targeting MMP-16 and ATG7 

  21. Upregulation of miR-92a contributes to blocking goblet cell metaplasia by targeting MUC5AC in asthma 

  22. A Selective α7 Nicotinic Acetylcholine Receptor Agonist, PNU-282987, Attenuates ILC2s Activation and Alternaria-Induced Airway Inflammation 

  23. Acupuncture inhibited airway inflammation and group 2 innate lymphoid cells in the lung in an ovalbumin-induced murine asthma model 

  24. Contribution of five major apple polyphenols in reducing peanut protein sensitization and alleviating allergencitiy of peanut by changing allergen structure 

  25. Substance P-induced lung inflammation in mice is mast cell dependent 

  26. N6-Methyladenosine Methylomic Landscape of Lung Tissues in Murine Acute Allergic Asthma 

  27. Wuling San and Xiao Chaihu Decoction affect airway inflammatory response and airway smooth muscle cell proliferation in mice with allergic asthma via miR-486-5p/AQP5 axis 

  28. Exosome membrane-modified M2 macrophages targeted nanomedicine: Treatment for allergic asthma 

  29. A novel iridoid glycoside leonuride (ajugol) attenuates airway inflammation and remodeling through inhibiting type-2 high cytokine/chemokine activity in OVA-induced asthmatic mice 

  30. Dioscin exhibits protective effects on in vivo and in vitro asthma models via suppressing TGF-β1/Smad2/3 and AKT pathways 

  31. Gene knockdown of CCR3 reduces eosinophilic inflammation and the Th2 immune response by inhibiting the PI3K/AKT pathway in allergic rhinitis mice 

  32. Lonicerin attenuates house dust mite-induced eosinophilic asthma through targeting Src/EGFR signaling 

  33. Metformin Ameliorates Inflammation and Airway Remodeling of Experimental Allergic Asthma in Mice by Restoring AMPKα Activity 

  34. Xanthatin alleviates airway inflammation in asthmatic mice by regulating the STAT3/NF-κB signaling pathway 

  35. Cold to Hot: Rational Design of a Minimalist Multifunctional Photo-immunotherapy Nanoplatform toward Boosting Immunotherapy Capability 

  36. Knockdown of Cadherin 26 Prevents the Inflammatory Responses of Allergic Rhinitis 

  37. Mdivi-1 alleviates atopic dermatitis through the inhibition of NLRP3 inflammasome 

  38. c-CBL/LCK/c-JUN/ETS1/CD28 axis restrains childhood asthma by suppressing Th2 differentiation 

  39. Enhancing protective immunity against bacterial infection via coating nano-Rehmannia glutinosa polysaccharide with outer membrane vesicles 

  40. Silencing TRIM8 alleviates allergic asthma and suppressing Th2 differentiation through inhibiting NF-κB/NLRP3 signaling pathway 

  41. Lentinan attenuates allergic airway inflammation and epithelial barrier dysfunction in asthma via inhibition of the PI3K/AKT/NF-κB pathway 

  42. Necroptosis plays a role in TL1A-induced airway inflammation and barrier damage in asthma 

  43. Dietary Fiber-Derived Microbial Butyrate Suppresses ILC2-Dependent Airway Inflammation in COPD 

  44. Tumor cells inhibit the activation of ILC2s through up-regulating PD-1 expression 

  45. Jiawei Yanghe Decoction attenuate allergic airway inflammation by suppressing group 2 innate lymphoid cells responses 

  46. AAV-delivered muscone-induced transgene system for treating chronic diseases in mice via inhalation 

  47. Radical S-adenosyl methionine domain-containing 2, a potential target of D-tryptophan in asthma treatment, regulates T helper cell type 1/2 balance 

  48. Edaravone alleviated allergic airway inflammation by inhibiting oxidative stress and endoplasmic reticulum stress 

  49. Baiheqingjin formula reduces inflammation in mice with asthma by inhibiting the PI3K/AKT/NF-κb signaling pathway 

  50. Lonicerin alleviates ovalbumin-induced asthma of mice via inhibiting EZH2/NF-κB signaling pathway 

  51. Mitochondrial dynamics modulate the allergic inflammation in a murine model of allergic rhinitis 

  52. Effects of total alkaloids from Alstonia scholaris (L.) R. Br. on ovalbumin-induced asthma mice 

  53. Effect of Peanut Protein Treated with Alkaline Protease and Flavorzyme on BALB/c Mice 

  54. ADP Ribosylation Factor 6 Relieves Airway Inflammation and Remodeling by Inhibiting Ovalbumin Induced-Epithelial Mesenchymal Transition in Experimental Asthma, Possibly by Regulating of E2F Transcription Factor 8 

  55. Heat-Killed Bifidobacterium longum BBMN68 in Pasteurized Yogurt Alleviates Mugwort Pollen-Induced Allergic Airway Responses through Gut Microbiota Modulation in a Murine Model 

  56. Transcriptomics reveals apigenin alleviates airway inflammation and epithelial cell apoptosis in allergic asthma via MAPK pathway 

  57. Silencing SOX11 Alleviates Allergic Rhinitis by Inhibiting Epithelial-Derived Cytokines 

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