EK205

Mouse IL-5 ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：IL5, IL-5, TRF
  • 种属：小鼠 (Mouse)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：血清血浆：10 μL；细胞培养上清：100 μL
  • 灵敏度：0.78 pg/mL
  • 检测范围：15.63 pg/mL - 1000 pg/mL
  • 回收率：78% - 115%

在售SKU:70-EK205-48, 70-EK205-96

描述

商品名

Mouse IL-5 ELISA Kit (小鼠白细胞介素5 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：5.2% - 7.2%；板间变异系数：4.3% - 7.4%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

血清血浆：10 μL；细胞培养上清：100 μL
灵敏度

0.78 pg/mL
检测范围

15.63 pg/mL - 1000 pg/mL
回收率

78% - 115%
平均回收率

1
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IL-5单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠IL-5 冻干标准品
  • IL-5检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-5抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-5与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-5的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IL-5 ELISA Kit (小鼠白细胞介素5 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 IL5, IL-5, TRF 靶点信息

IL5 分子靶点信息概述

  • 分子名：IL5, interleukin 5
  • 基因家族：Interleukins
  • 别名：IL-5; EDF; TRF
  • 全称：interleukin-5; T-cell replacing factor; B cell differentiation factor I; eosinophil differentiation factor; colony-stimulating factor, eosinophil; interleukin 5 (colony-stimulating factor, eosinophil)

IL5 分子靶点综述

白细胞介素5(IL-5)是造血家族Th2细胞因子成员，由115个氨基酸组成。与该细胞因子家族其它成员(IL-3和GM-CSF)不同，该糖蛋白的活化形式是同源二聚体。IL-5由Th2细胞和肥大细胞分泌。它对B细胞以及其它类型的细胞产生的生物学活性表明，IL-5对Th2细胞的活性具有重要的功能性作用。IL-5通过与其受体结合，刺激B细胞生长，提高免疫球蛋白分泌。它也是活化嗜酸性粒细胞的关键因子。IL-5一直以来与多种变态疾病相关，包括变应性鼻炎和哮喘，在循环组织、气管组织中观察到大幅增加的IL-5，而痰嗜酸性粒细胞则减少。

小鼠 Mouse Il5 分子靶点信息

小鼠 Mouse Il5 靶点分子功能（预测）

Enables cytokine activity. Involved in positive regulation of DNA-binding transcription factor activity; positive regulation of immunoglobulin production; and positive regulation of transcription, DNA-templated. Acts upstream of or within positive regulation of peptidyl-tyrosine phosphorylation. Located in extracellular space. Is expressed in colon; thymus; and thymus primordium. Human ortholog(s) of this gene implicated in asthma. Orthologous to human IL5 (interleukin 5).

操作步骤


下载本产品说明书

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

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引用文献

文章目录[隐藏]


  1. Small Proline-Rich Protein 3 Regulates IL-33/ILC2 Axis to Promote Allergic Airway Inflammation 

  2. Effects of divalent cations on the physical, conformational and immunological properties of bovine allergen β-lactoglobulin aggregates 

  3. Mechanistic study of salidroside on ovalbumin-induced asthmatic model mice based on untargeted metabolomics analysis 

  4. RETRACTED ARTICLE: Down-regulation of microRNA-342-5p or Up-regulation of Wnt3a Inhibits Angiogenesis and Maintains Atherosclerotic Plaque Stability in Atherosclerosis Mice 

  5. LncRNA AK085865 depletion ameliorates asthmatic airway inflammation by modulating macrophage polarization 

  6. HMGB1-induced ILC2s activate dendritic cells by producing IL-9 in asthmatic mouse model 

  7. Mucosal immunity of mannose-modified chitosan microspheres loaded with the nontyepable Haemophilus influenzae outer membrane protein P6 in BALB/c mice 

  8. Chrysophanol attenuates airway inflammation and remodeling through nuclear factor-kappa B signaling pathway in asthma 

  9. Louki Zupa decoction attenuates the airway inflammation in acute asthma mice induced by ovalbumin through IL-33/ST2-NF-κB/GSK3β/mTOR signalling pathway 

  10. TMT-based quantitative proteomics reveals suppression of SLC3A2 and ATP1A3 expression contributes to the inhibitory role of acupuncture on airway inflammation in an OVA-induced mouse asthma model 

  11. Icariside II attenuates eosinophils-induced airway inflammation and remodeling via inactivation of NF-κB and STAT3 in an asthma mouse model 

  12. Environmental allergens house dust mite‑induced asthma is associated with ferroptosis in the lungs 

  13. Melatonin modulates airway smooth muscle cell phenotype by targeting the STAT3/Akt/GSK-3β pathway in experimental asthma 

  14. Long non-coding RNA NKILA alleviates airway inflammation in asthmatic mice by promoting M2 macrophage polarization and inhibiting the NF-κB pathway 

  15. Solid lipid nanoparticle delivery of rhynchophylline enhanced the efficiency of allergic asthma treatment via the upregulation of suppressor of cytokine signaling 1 by repressing the p38 signaling pathway 

  16. Quantitative proteomic profiling of targeted proteins associated with Loki Zupa Decoction Treatment in OVA-Induced asthmatic mice 

  17. Rhynchophylline attenuates allergic bronchial asthma by inhibiting transforming growth factor‑β1‑mediated Smad and mitogen‑activated protein kinase signaling transductions in vivo and in vitro 

  18. Branched-chain amino acid transaminase 1 inhibition attenuates childhood asthma in mice by effecting airway remodeling and autophagy 

  19. TMT-Based Quantitative Proteomic Analysis Reveals Downregulation of ITGAL and Syk by the Effects of Cycloastragenol in OVA-Induced Asthmatic Mice 

  20. Isorhynchophylline exerts anti-asthma effects in mice by inhibiting the proliferation of airway smooth muscle cells: The involvement of miR-200a-mediated FOXC1/NF-κB pathway 

  21. Suppression of Hippo Pathway by Food Allergen Exacerbates Intestinal Epithelia Instability and Facilitates Hypersensitivity 

  22. Therapeutic Effect of Renifolin F on Airway Allergy in an Ovalbumin-Induced Asthma Mouse Model In Vivo 

  23. Jia-Wei-Yu-Ping-Feng-San Attenuates Group 2 Innate Lymphoid Cell-Mediated Airway Inflammation in Allergic Asthma 

  24. CpG-ODNs and Budesonide Act Synergistically to Improve Allergic Responses in Combined Allergic Rhinitis and Asthma Syndrome Induced by Chronic Exposure to Ovalbumin by Modulating the TSLP-DC-OX40L Axis 

  25. Cycloastragenol alleviates airway inflammation in asthmatic mice by inhibiting autophagy 

  26. The Involvement of Type 2 Innate Lymphoid Cells in Airway Inflammation of Asthma 

  27. Against NF-κB/thymic stromal lymphopoietin signaling pathway, catechin alleviates the inflammation in allergic rhinitis 

  28. Ginseng stem-leaf saponins in combination with selenium enhance immune responses to an attenuated pseudorabies virus vaccine 

  29. MiRNA-192-5p attenuates airway remodeling and autophagy in asthma by targeting MMP-16 and ATG7 

  30. Upregulation of miR-92a contributes to blocking goblet cell metaplasia by targeting MUC5AC in asthma 

  31. A Selective α7 Nicotinic Acetylcholine Receptor Agonist, PNU-282987, Attenuates ILC2s Activation and Alternaria-Induced Airway Inflammation 

  32. Acupuncture inhibited airway inflammation and group 2 innate lymphoid cells in the lung in an ovalbumin-induced murine asthma model 

  33. Contribution of five major apple polyphenols in reducing peanut protein sensitization and alleviating allergencitiy of peanut by changing allergen structure 

  34. Substance P-induced lung inflammation in mice is mast cell dependent 

  35. N6-Methyladenosine Methylomic Landscape of Lung Tissues in Murine Acute Allergic Asthma 

  36. Wuling San and Xiao Chaihu Decoction affect airway inflammatory response and airway smooth muscle cell proliferation in mice with allergic asthma via miR-486-5p/AQP5 axis 

  37. Exosome membrane-modified M2 macrophages targeted nanomedicine: Treatment for allergic asthma 

  38. Untargeted Metabolomic Profiling Reveals Changes in Gut Microbiota and Mechanisms of Its Regulation of Allergy in OVA-Sensitive BALB/c Mice 

  39. Protective Effects of Platycodin D3 on Airway Remodeling and Inflammation via Modulating MAPK/NF-κB Signaling Pathway in Asthma Mice 

  40. Proteomics analysis reveals suppression of IL-17 signaling pathways contributed to the therapeutic effects of Jia-Wei Bu-Shen-Yi-Qi formula in a murine asthma model 

  41. Syndecan-1 Amplifies Ovalbumin-Induced Airway Remodeling by Strengthening TGFβ1/Smad3 Action 

  42. The TL1A-DR3 Axis in Asthma: Membrane-Bound and Secreted TL1A Co-Determined the Development of Airway Remodeling 

  43. A novel iridoid glycoside leonuride (ajugol) attenuates airway inflammation and remodeling through inhibiting type-2 high cytokine/chemokine activity in OVA-induced asthmatic mice 

  44. Dioscin exhibits protective effects on in vivo and in vitro asthma models via suppressing TGF-β1/Smad2/3 and AKT pathways 

  45. Gene knockdown of CCR3 reduces eosinophilic inflammation and the Th2 immune response by inhibiting the PI3K/AKT pathway in allergic rhinitis mice 

  46. Lonicerin attenuates house dust mite-induced eosinophilic asthma through targeting Src/EGFR signaling 

  47. Metformin Ameliorates Inflammation and Airway Remodeling of Experimental Allergic Asthma in Mice by Restoring AMPKα Activity 

  48. Xanthatin alleviates airway inflammation in asthmatic mice by regulating the STAT3/NF-κB signaling pathway 

  49. Cold to Hot: Rational Design of a Minimalist Multifunctional Photo-immunotherapy Nanoplatform toward Boosting Immunotherapy Capability 

  50. Activation of STAT6 by intranasal allergens correlated with the development of eosinophilic chronic rhinosinusitis in a mouse model. 

  51. Knockdown of Cadherin 26 Prevents the Inflammatory Responses of Allergic Rhinitis 

  52. Mdivi-1 alleviates atopic dermatitis through the inhibition of NLRP3 inflammasome 

  53. c-CBL/LCK/c-JUN/ETS1/CD28 axis restrains childhood asthma by suppressing Th2 differentiation 

  54. Enhancing protective immunity against bacterial infection via coating nano-Rehmannia glutinosa polysaccharide with outer membrane vesicles 

  55. Silencing TRIM8 alleviates allergic asthma and suppressing Th2 differentiation through inhibiting NF-κB/NLRP3 signaling pathway 

  56. Lentinan attenuates allergic airway inflammation and epithelial barrier dysfunction in asthma via inhibition of the PI3K/AKT/NF-κB pathway 

  57. Protective Effects of Recombined Mussel Adhesive Protein against AD Skin Inflammation in Mice 

  58. ETS1 and RBPJ transcriptionally regulate METTL14 to suppress TGF-β1-induced epithelial-mesenchymal transition in human bronchial epithelial cells 

  59. Necroptosis plays a role in TL1A-induced airway inflammation and barrier damage in asthma 

  60. Dietary Fiber-Derived Microbial Butyrate Suppresses ILC2-Dependent Airway Inflammation in COPD 

  61. Tumor cells inhibit the activation of ILC2s through up-regulating PD-1 expression 

  62. Jiawei Yanghe Decoction attenuate allergic airway inflammation by suppressing group 2 innate lymphoid cells responses 

  63. Preparation of Budesonide-Loaded Liposomal Nanoparticles for Pulmonary Delivery and Their Therapeutic Effect in OVA-Induced Asthma in Mice 

  64. Edaravone alleviated allergic airway inflammation by inhibiting oxidative stress and endoplasmic reticulum stress 

  65. Baiheqingjin formula reduces inflammation in mice with asthma by inhibiting the PI3K/AKT/NF-κb signaling pathway 

  66. Lonicerin alleviates ovalbumin-induced asthma of mice via inhibiting EZH2/NF-κB signaling pathway 

  67. Plumbagin alleviates obesity-related asthma: Targeting inflammation, oxidative stress, and the AMPK pathway 

  68. Mitochondrial dynamics modulate the allergic inflammation in a murine model of allergic rhinitis 

  69. Effects of total alkaloids from Alstonia scholaris (L.) R. Br. on ovalbumin-induced asthma mice 

  70. Effect of Peanut Protein Treated with Alkaline Protease and Flavorzyme on BALB/c Mice 

  71. ADP Ribosylation Factor 6 Relieves Airway Inflammation and Remodeling by Inhibiting Ovalbumin Induced-Epithelial Mesenchymal Transition in Experimental Asthma, Possibly by Regulating of E2F Transcription Factor 8 

  72. Heat-Killed Bifidobacterium longum BBMN68 in Pasteurized Yogurt Alleviates Mugwort Pollen-Induced Allergic Airway Responses through Gut Microbiota Modulation in a Murine Model 

  73. Transcriptomics reveals apigenin alleviates airway inflammation and epithelial cell apoptosis in allergic asthma via MAPK pathway 

  74. A non-invasive strategy for suppressing asthmatic airway inflammation and remodeling: Inhalation of nebulized hypoxic hUCMSC-derived extracellular vesicles 

  75. Silencing SOX11 Alleviates Allergic Rhinitis by Inhibiting Epithelial-Derived Cytokines 

  76. Collagen Triple Helix Repeat Containing 1 Deficiency Protects Against Airway Remodeling and Inflammation in Asthma Models In Vivo and In Vitro 

  77. 支气管肺泡灌洗液 BALF 组织细胞

    Small Proline-Rich Protein 3 Regulates IL-33/ILC2 Axis to Promote Allergic Airway Inflammation 

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