EK223

Mouse IL-23 ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：IL23A, IL-23, SGRF
  • 种属：小鼠 (Mouse)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：20 μL
  • 灵敏度：0.31 pg/mL
  • 检测范围：1.56 pg/mL - 100 pg/mL
  • 回收率：80% - 99%

在售SKU:70-EK223-48, 70-EK223-96

描述

商品名

Mouse IL-23 ELISA Kit (小鼠白细胞介素23 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：3.0% - 5.4%；板间变异系数：4.4% - 6.4%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

20 μL
灵敏度

0.31 pg/mL
检测范围

1.56 pg/mL - 100 pg/mL
回收率

80% - 99%
平均回收率

0.91
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IL-23单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠IL-23冻干标准品
  • IL-23检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-23抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-23与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-23的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IL-23 ELISA Kit (小鼠白细胞介素23 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 IL23A, IL-23, SGRF 靶点信息

IL23A 分子靶点信息概述

  • 分子名：IL23A, interleukin 23 subunit alpha
  • 基因家族：Interleukins
  • 别名：SGRF; IL23P19; IL-23; IL-23A; P19
  • 全称：interleukin-six, G-CSF related factor; interleukin 23, alpha subunit p19

IL23A 分子靶点综述

白细胞介素23(IL-23)是一种由IL-12p40亚基和IL-23p19亚基组成的异二聚体细胞因子，其中p40亚基是IL-23与IL-12共用的。IL-23的功能性受体已被鉴定，由IL-12Rβ1和IL-23R组成。IL-23在1型极化T细胞的免疫应答中起作用。虽然IL-12可强有力激活幼稚T细胞，但是最初的报道认为IL-23可优先作用于记忆T细胞，促进其分泌IFN-γ和增殖，这表明IL-23在控制细菌感染上有重要作用。IL-23被进一步描述为是一种控制周围组织炎症的关键细胞因子。p19的过表达与多个器官以及包含皮肤在内的上皮组织的炎症有关。IL-23参与中枢神经系统和自身免疫性疾病的炎症。

小鼠 Mouse Il23a 分子靶点信息

小鼠 Mouse Il23a 靶点分子功能（预测）

Contributes to interleukin-23 receptor binding activity. Involved in positive regulation of defense response to virus by host; positive regulation of immune response; and positive regulation of neutrophil chemotaxis. Acts upstream of or within T cell proliferation and positive regulation of transcription by RNA polymerase II. Located in extracellular space. Part of interleukin-23 complex. Is expressed in decidua; decidua basalis; embryo; and extraembryonic component. Orthologous to human IL23A (interleukin 23 subunit alpha).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. RORγt inhibitor SR1001 alleviates acute pancreatitis by suppressing pancreatic IL-17-producing Th17 and γδ-T cells in mice with ceruletide-induced pancreatitis 

  2. Berberine-Loaded Carboxylmethyl Chitosan Nanoparticles Ameliorate DSS-Induced Colitis and Remodel Gut Microbiota in Mice 

  3. Abietic acid ameliorates psoriasis-like inflammation and modulates gut microbiota in mice 

  4. Potential mechanism of oral baicalin treating psoriasis via suppressing Wnt signaling pathway and inhibiting Th17/IL-17 axis by activating PPARγ 

  5. Mechanism of miRNA-based Aconitum leucostomum Worosch. Monomer inhibition of bone marrow-derived dendritic cell maturation 

  6. In vivo self-assembled siRNA as a modality for combination therapy of ulcerative colitis 

  7. TRIM31 facilitates K27-linked polyubiquitination of SYK to regulate antifungal immunity 

  8. A novel hyaluronic acid-based dissolving microneedle patch loaded with ginsenoside Rg3 liposome for effectively alleviate psoriasis 

  9. Terrestrosin D ameliorates skin lesions in an imiquimod-induced psoriasis-like murine model by inhibiting the interaction between Substance P and Dendritic cells 

  10. Inhibition of microRNA-155 attenuates concanavalin-A-induced autoimmune hepatitis by regulating Treg/Th17 cell differentiation 

  11. Enhanced antitumor effect of combination of annexin A1 knockdown and bortezomib treatment in multiple myeloma in vitro and in vivo 

  12. Juglone Suppresses Inflammation and Oxidative Stress in Colitis Mice 

  13. Rebamipide ophthalmic solution modulates the ratio of T helper cell 17/regulatory T cells in dry eye disease mice 

  14. RAS-association domain family 1A regulates the abnormal cell proliferation in psoriasis via inhibition of Yes-associated protein 

  15. Triptolide inhibits Th17 differentiation via controlling PKM2-mediated glycolysis in rheumatoid arthritis 

  16. Human IL-23R Cytokine-Binding Homology Region-Fc Fusion Protein Ameliorates Psoriasis via the Decrease of Systemic Th17 and ILC3 Cell Responses 

  17. Celastrol alleviates atopic dermatitis by regulating Ezrin-mediated mitochondrial fission and fusion 

  18. Thymic Stromal Lymphopoietin Activates Mouse Dendritic Cells Through the JAK/SYK Pathway in Promoting Th17 Response in Psoriasis 

  19. Accurate network pharmacology and novel ingredients formula of herbal targeting estrogen signaling for psoriasis intervention 

  20. GLP-1 receptor agonists alleviate colonic inflammation by modulating intestinal microbiota and the function of group 3 innate lymphoid cells 

  21. Photothermal Microneedle Hydrogel Patch for Refractory Soft Tissue Injuries through Thermosensitized Anti-Inflammaging Modulation 

  22. SGLT2 knockdown restores the Th17/Treg balance and suppresses diabetic nephropathy in db/db mice by regulating SGK1 via Na+ 

  23. MiR-326 sponges TET2 triggering imbalance of Th17/Treg differentiation to exacerbate pyroptosis of hepatocytes in concanavalin A-induced autoimmune hepatitis 

  24. Transcutaneous Immunotherapy for RNAi: A Cascade-Responsive Decomposable Nanocomplex Based on Polyphenol-Mediated Framework Nucleic Acid in Psoriasis 

  25. 皮肤 皮肤损伤组织 组织匀浆 血清

    Potential shared therapeutic and hepatotoxic mechanisms of Tripterygium wilfordii polyglycosides treating three kinds of autoimmune skin diseases by regulating IL-17 signaling pathway and Th17 cell differentiation 

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