EK222

Mouse IL-22 ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：IL22, IL-22, ILTIF, IL-TIF
  • 种属：小鼠 (Mouse)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：血清血浆：20 μL；细胞培养上清：100 μL
  • 灵敏度：0.74 pg/mL
  • 检测范围：15.63 pg/mL - 1000 pg/mL
  • 回收率：85% - 118%

在售SKU:70-EK222/2-48, 70-EK222/2-96

描述

商品名

Mouse IL-22 ELISA Kit (小鼠白细胞介素22 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：2.9% - 7.9%；板间变异系数：3.1% - 5.4%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

血清血浆：20 μL；细胞培养上清：100 μL
灵敏度

0.74 pg/mL
检测范围

15.63 pg/mL - 1000 pg/mL
回收率

85% - 118%
平均回收率

0.94
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IL-22单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠IL-22冻干标准品
  • IL-22 检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-22抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-22与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-22的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IL-22 ELISA Kit (小鼠白细胞介素22 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 IL22, IL-22, ILTIF, IL-TIF 靶点信息

IL22 分子靶点信息概述

  • 分子名：IL22, interleukin 22
  • 基因家族：Interleukins
  • 别名：ILTIF; IL-21; zcyto18; IL-TIF; IL-D110; TIFa; TIFIL-23; IL-22; MGC79382; MGC79384
  • 全称：IL-10-related T-cell-derived inducible factor

IL22 分子靶点综述

白细胞介素22(IL-22)是IL-10家族或者IL-10超家族(包括IL-19、IL-20、IL-24和IL-26)细胞因子中的一员，是细胞炎症反应的一类有效调节者。它与这个家族的其它成员，IL-10、IL-26、IL-28A/B和IL-29在细胞信号传导中共同使用IL-10R2。IL-22由活化的DC和T细胞产生，启动对抗病原菌特别是来源于上皮细胞如呼吸道和肠道上皮细胞的先天免疫应答。银屑病患者的血浆中表现出强烈升高的IL-22水平，这与疾病的严重程度相关。在由伴刀豆凝集素A(ConA)诱导的T细胞介导的肝炎中，IL-22可作为肝细胞的存活因子发挥保护作用。与IFN-γ和IL-17相反，克罗恩氏病患者血液中存在大量的IL-22。

小鼠 Mouse Il22 分子靶点信息

小鼠 Mouse Il22 靶点分子功能（预测）

Predicted to enable cytokine activity. Acts upstream of or within several processes, including negative regulation of inflammatory response; reactive oxygen species metabolic process; and regulation of tyrosine phosphorylation of STAT protein. Predicted to be located in extracellular space. Is expressed in retina and skin. Used to study liposarcoma. Human ortholog(s) of this gene implicated in asthma. Orthologous to human IL22 (interleukin 22).

操作步骤


下载本产品说明书

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

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【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. IL-22 ameliorated cardiomyocyte apoptosis in cardiac ischemia/reperfusion injury by blocking mitochondrial membrane potential decrease, inhibiting ROS and cytochrome C 

  2. Orally administered mesoporous silica capped with the cucurbit[8]uril complex to combat colitis and improve intestinal homeostasis by targeting the gut microbiota 

  3. Catalpol ameliorates psoriasis-like phenotypes via SIRT1 mediated suppression of NF-κB and MAPKs signaling pathways 

  4. Cimifugin ameliorates imiquimod-induced psoriasis by inhibiting oxidative stress and inflammation via NF-κB/MAPK pathway 

  5. Tethering Interleukin-22 to Apolipoprotein A-I Ameliorates Mice from Acetaminophen-induced Liver Injury 

  6. In vivo hepatocellular expression of interleukin-22 using penetratin-based hybrid nanoparticles as potential anti-hepatitis therapeutics 

  7. Interleukin-22 Attenuated Renal Tubular Injury in Aristolochic Acid Nephropathy via Suppressing Activation of NLRP3 Inflammasome 

  8. Interleukin-22 attenuates allergic airway inflammation in ovalbumin-induced asthma mouse model 

  9. Thymopentin ameliorates dextran sulfate sodium-induced colitis by triggering the production of IL-22 in both innate and adaptive lymphocytes 

  10. An elevated deoxycholic acid level induced by high-fat feeding damages intestinal stem cells by reducing the ileal IL-22 

  11. Structure Characterization of Honey-Processed Astragalus Polysaccharides and Its Anti-Inflammatory Activity In Vitro 

  12. NOD-Like Receptor Protein 3 Inflammasome-Dependent IL-1β Accelerated ConA-Induced Hepatitis 

  13. IL-22 ameliorates LPS-induced acute liver injury by autophagy activation through ATF4-ATG7 signaling 

  14. Beneficial effects of Dendrobium officinale National Herbal Drink on metabolic immune crosstalk via regulate SCFAs-Th17/Treg 

  15. Accurate network pharmacology and novel ingredients formula of herbal targeting estrogen signaling for psoriasis intervention 

  16. GLP-1 receptor agonists alleviate colonic inflammation by modulating intestinal microbiota and the function of group 3 innate lymphoid cells 

  17. Exopolysaccharides Produced by Bifidobacterium longum subsp. longum YS108R Ameliorates DSS-Induced Ulcerative Colitis in Mice by Improving the Gut Barrier and Regulating the Gut Microbiota 

  18. Disease-specific corona mediated co-delivery of MTX and siRNA-TNFα by a polypeptide nanoplatform with antigen-scavenging functions in psoriasis 

  19. Discovery of novel carboxylesterase 2 inhibitors for the treatment of delayed diarrhea and ulcerative colitis 

  20. Glucan from Dictyophora rubrovolvata boosts immunity by regulating AKT-STAT1 pathway to induce dendritic cells maturation and T cells differentiation 

  21. ILC2 regulates hyperoxia-induced lung injury via an enhanced Th17 cell response in the BPD mouse model 

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