EK213

Mouse IL-13 ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：IL13, ALRH, BHR1
  • 种属：小鼠 (Mouse)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：20 μL
  • 灵敏度：1.17 pg/mL
  • 检测范围：15.63 pg/mL - 1000 pg/mL
  • 回收率：89% - 112%

在售SKU:70-EK213/2-48, 70-EK213/2-96

描述

商品名

Mouse IL-13 ELISA Kit (小鼠白细胞介素13 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：2.3%-3.0%；板间变异系数：2.1% - 5.3%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

20 μL
灵敏度

1.17 pg/mL
检测范围

15.63 pg/mL - 1000 pg/mL
回收率

89% - 112%
平均回收率

1.02
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IL-13单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠IL-13冻干标准品
  • IL-13检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-13抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-13与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-13的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IL-13 ELISA Kit (小鼠白细胞介素13 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 IL13, ALRH, BHR1 靶点信息

IL13 分子靶点信息概述

  • 分子名：IL13, interleukin 13
  • 基因家族：Interleukins
  • 别名：P600; IL-13; ALRH; BHR1; MGC116786; MGC116788; MGC116789
  • 全称：allergic rhinitis; Bronchial hyperresponsiveness-1 (bronchial asthma)

IL13 分子靶点综述

白细胞介素13(IL-13)位于染色体5q31上，与IL-4具有30%的同源性和相似的结构。IL-13由许多细胞类型分泌产生，尤其是2型辅助性T细胞(Th2)。与密切相关的IL-4相似，IL-13对免疫细胞也具有多种功能。虽然IL-13主要与气道疾病的诱导相关，但是它还具有消炎特性。IL-13可诱导造血细胞的变化，但是这些效果与IL-4相比可能不那么重要。IL-13专门诱导寄生感染的器官的生理变化。它也可诱导过敏性肺部疾病的许多特征，包括气道高反应性、杯状细胞增殖和黏液高分泌，这些都会导致气道阻塞。此外，IL-13抑制原代培养衍生的巨噬细胞中HIV-1的复制，可作为体内单核细胞和巨噬细胞中抑制HIV感染的候选细胞因子。

小鼠 Mouse Il13 分子靶点信息

小鼠 Mouse Il13 靶点分子功能（预测）

Predicted to enable interleukin-13 receptor binding activity. Involved in positive regulation of cold-induced thermogenesis and positive regulation of macrophage activation. Acts upstream of or within cellular response to cytokine stimulus; inflammatory response; and positive regulation of immune effector process. Located in cytoplasm; external side of plasma membrane; and extracellular space. Is expressed in gut; liver; male reproductive gland or organ; and thymus. Used to study atopic dermatitis. Human ortholog(s) of this gene implicated in several diseases, including allergic disease (multiple); autoimmune disease (multiple); diffuse scleroderma; lung disease (multiple); and nose disease (multiple). Orthologous to human IL13 (interleukin 13).

操作步骤


下载本产品说明书

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

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引用文献

文章目录[隐藏]


  1. Small Proline-Rich Protein 3 Regulates IL-33/ILC2 Axis to Promote Allergic Airway Inflammation 

  2. ROS-responsive liposomes as an inhaled drug delivery nanoplatform for idiopathic pulmonary fibrosis treatment via Nrf2 signaling 

  3. Effects of divalent cations on the physical, conformational and immunological properties of bovine allergen β-lactoglobulin aggregates 

  4. Mechanistic study of salidroside on ovalbumin-induced asthmatic model mice based on untargeted metabolomics analysis 

  5. The Therapeutic Effects of Baicalin on Vitiligo Mice 

  6. GTS-21 Reduces Inflammation in Acute Lung Injury by Regulating M1 Polarization and Function of Alveolar Macrophages 

  7. LncRNA AK085865 depletion ameliorates asthmatic airway inflammation by modulating macrophage polarization 

  8. Long non-coding RNA growth arrest specific-5: a potential biomarker for early diagnosis of severe asthma 

  9. HMGB1-induced ILC2s activate dendritic cells by producing IL-9 in asthmatic mouse model 

  10. Chrysophanol attenuates airway inflammation and remodeling through nuclear factor-kappa B signaling pathway in asthma 

  11. Tfh Exosomes Derived from Allergic Rhinitis Promote DC Maturation Through miR-142-5p/CDK5/STAT3 Pathway 

  12. Louki Zupa decoction attenuates the airway inflammation in acute asthma mice induced by ovalbumin through IL-33/ST2-NF-κB/GSK3β/mTOR signalling pathway 

  13. TMT-based quantitative proteomics reveals suppression of SLC3A2 and ATP1A3 expression contributes to the inhibitory role of acupuncture on airway inflammation in an OVA-induced mouse asthma model 

  14. Artemisia annua water extract attenuates DNCB-induced atopic dermatitis by restraining Th2 cell mediated inflammatory responses in BALB/c mice 

  15. Icariside II attenuates eosinophils-induced airway inflammation and remodeling via inactivation of NF-κB and STAT3 in an asthma mouse model 

  16. Environmental allergens house dust mite‑induced asthma is associated with ferroptosis in the lungs 

  17. Melatonin modulates airway smooth muscle cell phenotype by targeting the STAT3/Akt/GSK-3β pathway in experimental asthma 

  18. Long non-coding RNA NKILA alleviates airway inflammation in asthmatic mice by promoting M2 macrophage polarization and inhibiting the NF-κB pathway 

  19. Solid lipid nanoparticle delivery of rhynchophylline enhanced the efficiency of allergic asthma treatment via the upregulation of suppressor of cytokine signaling 1 by repressing the p38 signaling pathway 

  20. Quantitative proteomic profiling of targeted proteins associated with Loki Zupa Decoction Treatment in OVA-Induced asthmatic mice 

  21. Rhynchophylline attenuates allergic bronchial asthma by inhibiting transforming growth factor‑β1‑mediated Smad and mitogen‑activated protein kinase signaling transductions in vivo and in vitro 

  22. Branched-chain amino acid transaminase 1 inhibition attenuates childhood asthma in mice by effecting airway remodeling and autophagy 

  23. TMT-Based Quantitative Proteomic Analysis Reveals Downregulation of ITGAL and Syk by the Effects of Cycloastragenol in OVA-Induced Asthmatic Mice 

  24. Isorhynchophylline exerts anti-asthma effects in mice by inhibiting the proliferation of airway smooth muscle cells: The involvement of miR-200a-mediated FOXC1/NF-κB pathway 

  25. Tissue-Resident Type 2 Innate Lymphoid Cells Arrest Alveolarization in Bronchopulmonary Dysplasia 

  26. Therapeutic Effect of Renifolin F on Airway Allergy in an Ovalbumin-Induced Asthma Mouse Model In Vivo 

  27. Bu-Shen-Yi-Qi formula ameliorates airway remodeling in murine chronic asthma by modulating airway inflammation and oxidative stress in the lung 

  28. The effect of neonatal maternal separation on short-chain fatty acids and airway inflammation in adult asthma mice 

  29. STING controls energy stress-induced autophagy and energy metabolism via STX17 

  30. Tacrolimus ameliorates thrombocytopenia in an ITP mouse model 

  31. Fine particulate matter increases airway hyperresponsiveness through kallikrein-bradykinin pathway 

  32. Triclosan exposure induced disturbance of gut microbiota and exaggerated experimental colitis in mice 

  33. Immunomodulatory activity of purified polysaccharides from Rubus chingii Hu fruits in lymphocytes and its molecular mechanisms 

  34. Jia-Wei-Yu-Ping-Feng-San Attenuates Group 2 Innate Lymphoid Cell-Mediated Airway Inflammation in Allergic Asthma 

  35. CpG-ODNs and Budesonide Act Synergistically to Improve Allergic Responses in Combined Allergic Rhinitis and Asthma Syndrome Induced by Chronic Exposure to Ovalbumin by Modulating the TSLP-DC-OX40L Axis 

  36. Cycloastragenol alleviates airway inflammation in asthmatic mice by inhibiting autophagy 

  37. The Involvement of Type 2 Innate Lymphoid Cells in Airway Inflammation of Asthma 

  38. Against NF-κB/thymic stromal lymphopoietin signaling pathway, catechin alleviates the inflammation in allergic rhinitis 

  39. MiRNA-192-5p attenuates airway remodeling and autophagy in asthma by targeting MMP-16 and ATG7 

  40. Upregulation of miR-92a contributes to blocking goblet cell metaplasia by targeting MUC5AC in asthma 

  41. A Selective α7 Nicotinic Acetylcholine Receptor Agonist, PNU-282987, Attenuates ILC2s Activation and Alternaria-Induced Airway Inflammation 

  42. Acupuncture inhibited airway inflammation and group 2 innate lymphoid cells in the lung in an ovalbumin-induced murine asthma model 

  43. Substance P-induced lung inflammation in mice is mast cell dependent 

  44. N6-Methyladenosine Methylomic Landscape of Lung Tissues in Murine Acute Allergic Asthma 

  45. Wuling San and Xiao Chaihu Decoction affect airway inflammatory response and airway smooth muscle cell proliferation in mice with allergic asthma via miR-486-5p/AQP5 axis 

  46. Exosome membrane-modified M2 macrophages targeted nanomedicine: Treatment for allergic asthma 

  47. Nrf2 regulates downstream genes by targeting miR-29b in severe asthma and the role of grape seed proanthocyanidin extract in a murine model of steroid-insensitive asthma 

  48. CpG-oligodeoxynucleotides may be effective for preventing ionizing radiation induced pulmonary fibrosis 

  49. Protective Effects of Platycodin D3 on Airway Remodeling and Inflammation via Modulating MAPK/NF-κB Signaling Pathway in Asthma Mice 

  50. Proteomics analysis reveals suppression of IL-17 signaling pathways contributed to the therapeutic effects of Jia-Wei Bu-Shen-Yi-Qi formula in a murine asthma model 

  51. Syndecan-1 Amplifies Ovalbumin-Induced Airway Remodeling by Strengthening TGFβ1/Smad3 Action 

  52. The TL1A-DR3 Axis in Asthma: Membrane-Bound and Secreted TL1A Co-Determined the Development of Airway Remodeling 

  53. A Neonatal Murine Model of Coxsackievirus A6 Infection for Evaluation of Antiviral and Vaccine Efficacy 

  54. A novel iridoid glycoside leonuride (ajugol) attenuates airway inflammation and remodeling through inhibiting type-2 high cytokine/chemokine activity in OVA-induced asthmatic mice 

  55. Follistatin-related protein 1 in asthma: miR-200b-3p interactions affect airway remodeling and inflammation phenotype 

  56. Dioscin exhibits protective effects on in vivo and in vitro asthma models via suppressing TGF-β1/Smad2/3 and AKT pathways 

  57. Metformin Ameliorates Inflammation and Airway Remodeling of Experimental Allergic Asthma in Mice by Restoring AMPKα Activity 

  58. Xanthatin alleviates airway inflammation in asthmatic mice by regulating the STAT3/NF-κB signaling pathway 

  59. Recombination of a dual-CAR-modified T lymphocyte to accurately eliminate pancreatic malignancy 

  60. Murine model of acute myocarditis and cerebral cortical neuron edema induced by coxsackievirus B4 

  61. Protective Efficacies of Formaldehyde-Inactivated Whole-Virus Vaccine and Antivirals in a Murine Model of Coxsackievirus A10 Infection 

  62. Tespa1 plays a role in the modulation of airway hyperreactivity through the IL-4/STAT6 pathway 

  63. Knockdown of Cadherin 26 Prevents the Inflammatory Responses of Allergic Rhinitis 

  64. Mdivi-1 alleviates atopic dermatitis through the inhibition of NLRP3 inflammasome 

  65. Mesenchymal stromal cells pretreated with proinflammatory cytokines enhance skin wound healing via IL-6-dependent M2 polarization 

  66. c-CBL/LCK/c-JUN/ETS1/CD28 axis restrains childhood asthma by suppressing Th2 differentiation 

  67. Silencing TRIM8 alleviates allergic asthma and suppressing Th2 differentiation through inhibiting NF-κB/NLRP3 signaling pathway 

  68. Lentinan attenuates allergic airway inflammation and epithelial barrier dysfunction in asthma via inhibition of the PI3K/AKT/NF-κB pathway 

  69. Protective Effects of Recombined Mussel Adhesive Protein against AD Skin Inflammation in Mice 

  70. ETS1 and RBPJ transcriptionally regulate METTL14 to suppress TGF-β1-induced epithelial-mesenchymal transition in human bronchial epithelial cells 

  71. Necroptosis plays a role in TL1A-induced airway inflammation and barrier damage in asthma 

  72. Dietary Fiber-Derived Microbial Butyrate Suppresses ILC2-Dependent Airway Inflammation in COPD 

  73. FOXK2 facilitates the airway remodeling during chronic asthma by promoting glycolysis in a SIRT2-dependent manner 

  74. Tumor cells inhibit the activation of ILC2s through up-regulating PD-1 expression 

  75. Silencing of forkhead box C1 reduces nasal epithelial barrier damage in mice with allergic rhinitis via epigenetically upregulating secreted frizzled-related protein 5 

  76. Jiawei Yanghe Decoction attenuate allergic airway inflammation by suppressing group 2 innate lymphoid cells responses 

  77. Radical S-adenosyl methionine domain-containing 2, a potential target of D-tryptophan in asthma treatment, regulates T helper cell type 1/2 balance 

  78. Preparation of Budesonide-Loaded Liposomal Nanoparticles for Pulmonary Delivery and Their Therapeutic Effect in OVA-Induced Asthma in Mice 

  79. Edaravone alleviated allergic airway inflammation by inhibiting oxidative stress and endoplasmic reticulum stress 

  80. Baiheqingjin formula reduces inflammation in mice with asthma by inhibiting the PI3K/AKT/NF-κb signaling pathway 

  81. Lonicerin alleviates ovalbumin-induced asthma of mice via inhibiting EZH2/NF-κB signaling pathway 

  82. Jiangqi Pingxiao formula regulates dendritic cell apoptosis in an autophagy-dependent manner through the AMPK/mTOR pathway in a murine model of OVA-induced asthma 

  83. Effects of total alkaloids from Alstonia scholaris (L.) R. Br. on ovalbumin-induced asthma mice 

  84. Surface Functional Modification by Ti3C2Tx MXene on PLLA Nanofibers for Optimizing Neural Stem Cell Engineering 

  85. ADP Ribosylation Factor 6 Relieves Airway Inflammation and Remodeling by Inhibiting Ovalbumin Induced-Epithelial Mesenchymal Transition in Experimental Asthma, Possibly by Regulating of E2F Transcription Factor 8 

  86. Schistosoma japonicum-derived peptide SJMHE1 ameliorates allergic symptoms and responses in mice with allergic rhinitis 

  87. Heat-Killed Bifidobacterium longum BBMN68 in Pasteurized Yogurt Alleviates Mugwort Pollen-Induced Allergic Airway Responses through Gut Microbiota Modulation in a Murine Model 

  88. Butyrate inhibits iILC2-mediated lung inflammation via lung-gut axis in chronic obstructive pulmonary disease (COPD) 

  89. Transcriptomics reveals apigenin alleviates airway inflammation and epithelial cell apoptosis in allergic asthma via MAPK pathway 

  90. A non-invasive strategy for suppressing asthmatic airway inflammation and remodeling: Inhalation of nebulized hypoxic hUCMSC-derived extracellular vesicles 

  91. Versatility of bacterial outer membrane vesicles in regulating intestinal homeostasis 

  92. Effects of Pine Pollen Polysaccharides and Sulfated Polysaccharides on Ulcerative Colitis and Gut Flora in Mice 

  93. Silencing SOX11 Alleviates Allergic Rhinitis by Inhibiting Epithelial-Derived Cytokines 

  94. Collagen Triple Helix Repeat Containing 1 Deficiency Protects Against Airway Remodeling and Inflammation in Asthma Models In Vivo and In Vitro 

  95. Regulation of ferroptosis and ACSL4-15LO1 pathway contributed to the anti-asthma effect of acupuncture 

  96. 支气管肺泡灌洗液 BALF 组织细胞

    Small Proline-Rich Protein 3 Regulates IL-33/ILC2 Axis to Promote Allergic Airway Inflammation 

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