EK212

Mouse IL-12p70 ELISA Kit检测试剂盒（酶联免疫吸附法）

X

¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：IL12, IL12B, NKSF, CLMF2
  • 种属：小鼠 (Mouse)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：20 μL
  • 灵敏度：3.58 pg/mL
  • 检测范围：31.25 pg/mL - 2000 pg/mL
  • 回收率：89% - 114%

在售SKU:70-EK212/3-48, 70-EK212/3-96

描述

商品名

Mouse IL-12p70 ELISA Kit (小鼠IL-12p70 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：5.2% - 9.4%；板间变异系数：6.5% - 7.9%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

20 μL
灵敏度

3.58 pg/mL
检测范围

31.25 pg/mL - 2000 pg/mL
回收率

89% - 114%
平均回收率

0.99
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IL-12p70单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠IL-12p70冻干标准品
  • IL-12p70检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-12p70抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-12p70与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-12p70的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IL-12p70 ELISA Kit (小鼠IL-12p70 ELISA试剂盒) - 标准曲线
标准曲线

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. C9–13 chlorinated paraffins cause immunomodulatory effects in adult C57BL/6 mice 

  2. S-allylmercaptocysteine inhibits mucin overexpression and inflammation via MAPKs and PI3K-Akt signaling pathways in acute respiratory distress syndrome 

  3. Remodeling immune microenvironment in periodontitis using resveratrol liposomes as an antibiotic-free therapeutic strategy 

  4. Dual Inhibition of Endoplasmic Reticulum Stress and Oxidation Stress Manipulates the Polarization of Macrophages under Hypoxia to Sensitize Immunotherapy 

  5. Tumor Microenvironment-Triggered Charge Reversal Polymetformin-Based Nanosystem Co-Delivered Doxorubicin and IL-12 Cytokine Gene for Chemo–Gene Combination Therapy on Metastatic Breast Cancer 

  6. Effect of Lactobacillus reuteri on intestinal microbiota and immune parameters: Involvement of sex differences 

  7. M1/M2 Macrophages Play Different Roles in Adipogenic Differentiation of PDGFRα+ Preadipocytes In Vitro 

  8. Amplified Cancer Immunotherapy of a Surface-Engineered Antigenic Microparticle Vaccine by Synergistically Modulating Tumor Microenvironment 

  9. LincRNA-p21 knockdown reversed tumor-associated macrophages function by promoting MDM2 to antagonize* p53 activation and alleviate breast cancer development 

  10. Different protein of Echinococcus granulosus stimulates dendritic induced immune response 

  11. Self-Assembled Immunostimulatory Tetrahedral Framework Nucleic Acid Vehicles for Tumor Chemo-immunotherapy 

  12. Effects of TLR4 on β2-glycoprotein I-induced bone marrow-derived dendritic cells maturation 

  13. A peptide inhibitor of macrophage migration in atherosclerosis purified from the leech Whitmania pigra 

  14. In vivo self-assembled siRNA as a modality for combination therapy of ulcerative colitis 

  15. Improved immune response to an attenuated pseudorabies virus vaccine by ginseng stem-leaf saponins (GSLS) in combination with thimerosal (TS) 

  16. Reactivation of dysfunctional dendritic cells by a stress-relieving nanosystem resets anti-tumor immune landscape 

  17. Erythrocyte Membrane-Enveloped Polymeric Nanoparticles as Nanovaccine for Induction of Antitumor Immunity against Melanoma 

  18. RETRACTED ARTICLE: Down-regulation of microRNA-342-5p or Up-regulation of Wnt3a Inhibits Angiogenesis and Maintains Atherosclerotic Plaque Stability in Atherosclerosis Mice 

  19. Intravenous injection of the oncolytic virus M1 awakens antitumor T cells and overcomes resistance to checkpoint blockade 

  20. Macrophage immunomodulatory activity of the cationic polymer modified PLGA nanoparticles encapsulating Alhagi honey polysaccharide 

  21. A Vaccination with Boosted Cross Presentation by ER-Targeted Antigen Delivery for Anti-Tumor Immunotherapy 

  22. Mannose-Modified PLGA Nanoparticles for Sustained and Targeted Delivery in Hepatitis B Virus Immunoprophylaxis 

  23. Critical roles of conventional dendritic cells in autoimmune hepatitis via autophagy regulation 

  24. Microneedle-assisted dendritic cell-targeted nanoparticles for transcutaneous DNA immunization 

  25. A Solution with Ginseng Saponins and Selenium as Vaccine Diluent to Increase Th1/Th2 Immune Responses in Mice 

  26. Ginseng Stem-Leaf Saponins in Combination with Selenium Promote the Immune Response in Neonatal Mice with Maternal Antibody 

  27. Toxoplasma gondii ROP18I inhibits host innate immunity through cGAS-STING signaling 

  28. Boosting Tumor Immunotherapy by Bioactive Nanoparticles via Ca2+ Interference Mediated TME Reprogramming and Specific PD-L1 Depletion 

  29. In situ photothermal nano-vaccine based on tumor cell membrane-coated black phosphorus-Au for photo-immunotherapy of metastatic breast tumors 

  30. Bacterial navigation for tumor targeting and photothermally-triggered bacterial ghost transformation for spatiotemporal drug release 

  31. Chitosan Nanovaccines as Efficient Carrier Adjuvant System for IL-12 with Enhanced Protection Against HBV 

  32. miR-130b-3p regulates M1 macrophage polarization via targeting IRF1 

  33. A non-bactericidal cathelicidin provides prophylactic efficacy against bacterial infection by driving phagocyte influx 

  34. Tacrolimus ameliorates thrombocytopenia in an ITP mouse model 

  35. Viperin deficiency promotes dendritic cell activation and function via NF-kappaB activation during Mycobacterium tuberculosis infection 

  36. Blockade of exosome generation by GW4869 inhibits the education of M2 macrophages in prostate cancer 

  37. Polyethylenimine-coated PLGA nanoparticles containing Angelica sinensis polysaccharide promote dendritic cells activation and associated molecular mechanisms 

  38. Terrestrosin D ameliorates skin lesions in an imiquimod-induced psoriasis-like murine model by inhibiting the interaction between Substance P and Dendritic cells 

  39. Combined Antitumor Effects of Sorafenib and GPC3-CAR T Cells in Mouse Models of Hepatocellular Carcinoma 

  40. A Cu9S5 nanoparticle-based CpG delivery system for synergistic photothermal-, photodynamic- and immunotherapy 

  41. A low-intensity focused ultrasound-assisted nanocomposite for advanced triple cancer therapy: local chemotherapy, therapeutic extracellular vesicles and combined immunotherapy 

  42. A nanoplatform to boost multi-phases of cancer-immunity-cycle for enhancing immunotherapy 

  43. Glutamine Metabolism-Regulated Nanoparticles to Enhance Chemoimmunotherapy by Increasing Antigen Presentation Efficiency 

  44. Targeted Delivery of miRNA 155 to Tumor Associated Macrophages for Tumor Immunotherapy 

  45. CD137 signaling induces macrophage M2 polarization in atherosclerosis through STAT6/PPARδ pathway 

  46. Sustained Antitumor Immunity Based on Persistent Luminescence Nanoparticles for Cancer Immunotherapy 

  47. Juglone Suppresses Inflammation and Oxidative Stress in Colitis Mice 

  48. Curdlan sulfate/O-linked quaternized chitosan nanoparticles acting as potential adjuvants promote multiple arms of immune responses 

  49. Bacterium-Mimicking Vector with Enhanced Adjuvanticity for Cancer Immunotherapy and Minimized Toxicity 

  50. Fluorous-Tagged Peptide Nanoparticles Ameliorate Acute Lung Injury via Lysosomal Stabilization and Inflammation Inhibition in Pulmonary Macrophages 

  51. Ginseng stem-leaf saponins in combination with selenium enhance immune responses to an attenuated pseudorabies virus vaccine 

  52. CpG-oligodeoxynucleotides may be effective for preventing ionizing radiation induced pulmonary fibrosis 

  53. Chinese yam polysaccharides PLGA-stabilized Pickering emulsion as an adjuvant system for PCV- 2 vaccine to enhance immune response 

  54. Design of a self-driven probiotic-CRISPR/Cas9 nanosystem for sono-immunometabolic cancer therapy 

  55. Transdermal delivery system based on heparin-modified graphene oxide for deep transportation, tumor microenvironment regulation, and immune activation 

  56. Alleviation of cyclophosphamide-induced immunosuppression in mice by naturally acetylated hemicellulose from bamboo shavings 

  57. Self-assembled tetrahedral framework nucleic acid mediates tumor-associated macrophage reprogramming and restores antitumor immunity 

  58. Immunopotentiation of Polysaccharides of Atractylodes macrocephala Koidz-loaded nanostructured lipid carriers as an adjuvant 

  59. Polyethylenimine-coated PLGA nanoparticles-encapsulated Angelica sinensis polysaccharide as an adjuvant to enhance immune responses 

  60. Polystyrene nanoparticles trigger the activation of p38 MAPK and apoptosis via inducing oxidative stress in zebrafish and macrophage cells 

  61. Berberine inhibits macrophage M1 polarization via AKT1/SOCS1/NF-κB signaling pathway to protect against DSS-induced colitis 

  62. Significantly increased anti-tumor activity of carcinoembryonic antigen-specific chimeric antigen receptor T cells in combination with recombinant human IL-12 

  63. Armored Inducible Expression of IL-12 Enhances Antitumor Activity of Glypican-3–Targeted Chimeric Antigen Receptor–Engineered T Cells in Hepatocellular Carcinoma 

  64. Enhancing protective immunity against bacterial infection via coating nano-Rehmannia glutinosa polysaccharide with outer membrane vesicles 

  65. Glypican-3-targeted macrophages delivering drug-loaded exosomes offer efficient cytotherapy in mouse models of solid tumours 

  66. A nanotheranostics with hypoxia-switchable fluorescence and photothermal effect for hypoxia imaging-guided immunosuppressive tumor microenvironment modulation 

  67. Localized light-triggered release macrophage cytopharmaceuticals containing O-nitrobenzyl group for enhanced solid tumor cell-chemotherapy 

  68. Astragaloside IV microfibers assembling into injectable 3D-scaffolds with intrinsic immunoactivity for enhanced tumor vaccine efficacy 

  69. Metal-ion-chelating phenylalanine nanostructures reverse immune dysfunction and sensitize breast tumour to immune checkpoint blockade 

  70. Protective Effects of Recombined Mussel Adhesive Protein against AD Skin Inflammation in Mice 

  71. Toxoplasma gondii bradyzoite-specific BAG1 is nonessential for cyst formation due to compensation by other heat-shock proteins 

  72. In situ reprogramming of tumor-associated macrophages enhances cancer immunotherapy 

  73. A polymeric nanoplatform enhances the cGAS-STING pathway in macrophages to potentiate phagocytosis for cancer immunotherapy 

  74. Dual-Functional Nanodroplet for Tumor Vasculature Ultrasound Imaging and Tumor Immunosuppressive Microenvironment Remodeling 

  75. Boron Dipyrromethene-Based Nanotheranostic System for Sonophotoassisted Therapy and Simultaneous Monitoring of Tumor Immune Microenvironment Reprogramming 

  76. Controlling the speed of antigens transport in dendritic cells improves humoral and cellular immunity for vaccine 

  77. Structural characterization and adjuvant activity of a water soluble polysaccharide from Poria cocos 

  78. Rare Earth Nanoprobes for Targeted Delineation of Triple Negative Breast Cancer and Enhancement of Radioimmunotherapy 

  79. Glycyrrhiza polysaccharides may have an antitumor effect in γδT cells through gut microbiota and TLRs/NF-κB pathway in mice 

  80. Preparation and characterization of curdlan-chitosan conjugate nanoparticles as mucosal adjuvants for intranasal influenza H1N1 subunit vaccine 

  81. Self-delivery photothermal-boosted-nanobike multi-overcoming immune escape by photothermal/chemical/immune synergistic therapy against HCC 

  82. Targeted knockdown of PGAM5 in synovial macrophages efficiently alleviates osteoarthritis 

  83. Biomimetic bacteria-derived nanoclusters enhance ferroptosis cancer immunotherapy through synergistic CRISPR-photothermo modulation 

  84. Matrix metalloproteinase-8 regulates dendritic cell tolerance in late polymicrobial sepsis via the nuclear factor kappa-B p65/β-catenin pathway 

  85. Solobacterium moorei promotes the progression of adenomatous polyps by causing inflammation and disrupting the intestinal barrier 

  86. PI3Kδ inhibition alleviates the brain injury during cerebral ischemia reperfusion via suppressing pericyte contraction in a TNF-α dependent manner 

  87. Cistanche deserticola polysaccharide-functionalized dendritic fibrous nano-silica as oral vaccine adjuvant delivery enhancing both the mucosal and systemic immunity 

  88. Inhalable metal-organic framework-mediated cuproptosis combined with PD-L1 checkpoint blockade for lung metastasis synergistic immunotherapy 

  89. Mnox Nanoenzyme Armed CAR-NK Cells Enhance Solid Tumor Immunotherapy by Alleviating the Immunosuppressive Microenvironment 

  90. Tumor Microenvironment Sequential Drug/Gene Delivery Nanosystem for Realizing Multistage Boosting of Cancer–Immunity Cycle on Cancer Immunotherapy 

  91. Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFA 

  92. Cell Communication-Mediated Selective Drug Delivery for Epitope-Specific Deletion of Alloreactive T Cells in Organ Transplant 

  93. Carrier-Free Nanovaccine: An Innovative Strategy for Ultrahigh Melanoma Neoantigen Loading 

  94. Immunostimulant Hydrogel-Guided Tumor Microenvironment Reprogramming to Efficiently Potentiate Macrophage-Mediated Cellular Phagocytosis for Systemic Cancer Immunotherapy 

  95. Liquid nitrogen frozen cells for chemotherapy drug delivery and vaccination of melanoma 

  96. Glucan from Dictyophora rubrovolvata boosts immunity by regulating AKT-STAT1 pathway to induce dendritic cells maturation and T cells differentiation 

  97. Melatonin attenuates lipopolysaccharide-induced immune dysfunction in dendritic cells 

  98. Alpha-linolenic acid improves nasal mucosa epithelial barrier function in allergic rhinitis by arresting CD4+ T cell differentiation via IL-4Rα-JAK2-STAT3 pathway 

  99. Temperature sensitive liposome based cancer nanomedicine enables tumour lymph node immune microenvironment remodelling 

  100. Targeting STING Activation by Antigen-Inspired MnO2 Nanovaccines Optimizes Tumor Radiotherapy 

  101. Dissolving Microneedle Arrays as a Hepatitis B Vaccine Delivery System Adjuvanted by APC-Targeted Poly (Lactic-co-Glycolic Acid) (PLGA) Nanoparticles 

  102. In vitro and in vivo exploration of the anti-atopic dermatitis mechanism of action of Tibetan medicine Qi-Sai-Er-Sang-Dang-Song decoction 

  103. A polymeric nanoplatform enhances the cGAS-STING pathway in macrophages to potentiate phagocytosis for cancer immunotherapy 

  104. A polymeric nanoplatform enhances the cGAS-STING pathway in macrophages to potentiate phagocytosis for cancer immunotherapy 

  105. 细胞培养上清 血清

    The Immunoenhancement Effects of Polyethylenimine-Modified Chinese Yam Polysaccharide-Encapsulated Plga Nanoparticles as an Adjuvant 

  106. 细胞培养上清

    Intravenous Injection of the Oncolytic Virus M1 Awakens Antitumor T Cells and Overcomes Resistance to Checkpoint Blockade 

  107. 组织匀浆 细胞培养上清 肿瘤 血清

    Bacterium-Mimicking Vector with Enhanced Adjuvanticity for Cancer Immunotherapy and Minimized Toxicity 

  108. RAW 264.7 单核巨噬细胞 细胞培养上清

    CD137 signaling induces macrophage M2 polarization in atherosclerosis through STAT6/PPARδ pathway 

评价

目前还没有评价

成为第一个“Mouse IL-12p70 ELISA Kit检测试剂盒（酶联免疫吸附法）” 的评价者

您的电子邮箱地址不会被公开。 必填项已用*标注

相关产品