EK212HSS

Mouse IL-12p70 High Sensitivity Standard (小鼠白细胞介素12 (IL-12) 高敏 标准品)

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¥180.00

  • 分子靶点:IL12, IL12B, NKSF, CLMF2
  • 种属:小鼠 (Mouse)
  • 试剂盒:EK212HS
  • 保存:短期4℃保存,长期-20℃保存
  • 运输条件:4℃蓝冰运输

在售SKU:70-EK212HSS


描述

本产品只包含标准品试剂,如需购买试剂盒请点击下图

商品名

Mouse IL-12p70 High Sensitivity Standard (小鼠白细胞介素12 (IL-12) 高敏 标准品)

组分

小鼠IL-12p70标准品

检测方法

双抗夹心法

样本类型

血清,血浆,细胞培养上清及其他生物学样本

板式

保存

短期4℃,长期-20℃保存

运输条件

4℃蓝冰运输

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-12p70抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的IL-12p70与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入信号增强剂孵育,洗涤去除未结合的物质后,再次加入Streptavidin-HRP。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-12p70的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IL-12p70 High Sensitivity ELISA Kit (小鼠白细胞介素12 (IL-12) 高敏 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 IL12B, NKSF, CLMF2 靶点信息

IL12B 分子靶点信息概述

  • 分子名:IL12B, interleukin 12B
  • 基因家族:Immunoglobulin like domain containing; Interleukins
  • 别名:CLMF; IL-12B; NKSF; CLMF2
  • 曾用名:NKSF2
  • 全称:natural killer cell stimulatory factor-2; cytotoxic lymphocyte maturation factor 2, p40; interleukin 12, p40; natural killer cell stimulatory factor, 40 kD subunit; interleukin-12 beta chain; IL12, subunit p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)

IL12B 分子靶点综述

白细胞介素12 (IL-12)由树突状细胞、巨噬细胞、中性粒细胞和人B淋巴母细胞株 (NC-37)在响应抗原刺激时自然产生。IL-12由4个а螺旋组成,是一个由2种独立基因--- IL-12A (p35)和IL-12B (p40)编码形成的异源二聚体细胞因子。蛋白合成之后可形成有生物活性的异源二聚体(p70)和同源二聚体p40。IL-12参与初始T细胞向Th1细胞分化的过程,作为T细胞刺激因子,它可刺激T细胞的生长及其功能发挥。IL-12具有抗血管生成活性,对自然杀伤细胞和T淋巴细胞活性有重要作用。同样地,IL-12与自身免疫相关。

小鼠 Mouse Il12b 分子靶点信息

小鼠 Mouse Il12b 靶点分子功能(预测)

Enables identical protein binding activity and interleukin-12 alpha subunit binding activity. Contributes to cytokine activity and cytokine receptor binding activity. Involved in positive regulation of T cell mediated cytotoxicity; positive regulation of T-helper 1 type immune response; and positive regulation of defense response to virus by host. Acts upstream of or within several processes, including cellular response to lipopolysaccharide; defense response to other organism; and positive regulation of interferon-gamma production. Located in cell surface; cytoplasm; and extracellular space. Part of interleukin-12 complex and interleukin-23 complex. Is expressed in colon. Human ortholog(s) of this gene implicated in asthma; chronic obstructive pulmonary disease; immunodeficiency 29; and salmonellosis. Orthologous to human IL12B (interleukin 12B).

操作步骤


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引用文献

文章目录[隐藏]


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  6. Amplified Cancer Immunotherapy of a Surface-Engineered Antigenic Microparticle Vaccine by Synergistically Modulating Tumor Microenvironment 

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  9. Self-Assembled Immunostimulatory Tetrahedral Framework Nucleic Acid Vehicles for Tumor Chemo-immunotherapy 

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  16. RETRACTED ARTICLE: Down-regulation of microRNA-342-5p or Up-regulation of Wnt3a Inhibits Angiogenesis and Maintains Atherosclerotic Plaque Stability in Atherosclerosis Mice 

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  89. Dissolving Microneedle Arrays as a Hepatitis B Vaccine Delivery System Adjuvanted by APC-Targeted Poly (Lactic-co-Glycolic Acid) (PLGA) Nanoparticles 

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