EK212HS

Mouse IL-12p70 High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥2,000.00 – ¥3,400.00

  • 分子靶点：IL12, IL12B, NKSF, CLMF2
  • 种属：小鼠 (Mouse)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：20 μL
  • 灵敏度：0.57 pg/mL
  • 检测范围：7.81 pg/mL - 500 pg/mL
  • 回收率：92% - 115%

在售SKU:70-EK212HS-48, 70-EK212HS-96

描述

商品名

Mouse IL-12p70 High Sensitivity ELISA Kit (小鼠白细胞介素12 (IL-12) 高敏 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：2.2% - 3.1%；板间变异系数：2.6% - 3.1%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

20 μL
灵敏度

0.57 pg/mL
检测范围

7.81 pg/mL - 500 pg/mL
回收率

92% - 115%
平均回收率

1.04
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IL-12p70单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠IL-12p70冻干标准品
  • IL-12p70检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 信号增强剂浓缩液
  • 信号增强剂稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-12p70抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的IL-12p70与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-12p70的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IL-12p70 High Sensitivity ELISA Kit (小鼠白细胞介素12 (IL-12) 高敏 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 IL12B, NKSF, CLMF2 靶点信息

IL12B 分子靶点信息概述

  • 分子名：IL12B, interleukin 12B
  • 基因家族：Immunoglobulin like domain containing; Interleukins
  • 别名：CLMF; IL-12B; NKSF; CLMF2
  • 曾用名：NKSF2
  • 全称：natural killer cell stimulatory factor-2; cytotoxic lymphocyte maturation factor 2, p40; interleukin 12, p40; natural killer cell stimulatory factor, 40 kD subunit; interleukin-12 beta chain; IL12, subunit p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)

IL12B 分子靶点综述

白细胞介素12 (IL-12)由树突状细胞、巨噬细胞、中性粒细胞和人B淋巴母细胞株 (NC-37)在响应抗原刺激时自然产生。IL-12由4个а螺旋组成，是一个由2种独立基因--- IL-12A (p35)和IL-12B (p40)编码形成的异源二聚体细胞因子。蛋白合成之后可形成有生物活性的异源二聚体(p70)和同源二聚体p40。IL-12参与初始T细胞向Th1细胞分化的过程，作为T细胞刺激因子，它可刺激T细胞的生长及其功能发挥。IL-12具有抗血管生成活性，对自然杀伤细胞和T淋巴细胞活性有重要作用。同样地，IL-12与自身免疫相关。

小鼠 Mouse Il12b 分子靶点信息

小鼠 Mouse Il12b 靶点分子功能（预测）

Enables identical protein binding activity and interleukin-12 alpha subunit binding activity. Contributes to cytokine activity and cytokine receptor binding activity. Involved in positive regulation of T cell mediated cytotoxicity; positive regulation of T-helper 1 type immune response; and positive regulation of defense response to virus by host. Acts upstream of or within several processes, including cellular response to lipopolysaccharide; defense response to other organism; and positive regulation of interferon-gamma production. Located in cell surface; cytoplasm; and extracellular space. Part of interleukin-12 complex and interleukin-23 complex. Is expressed in colon. Human ortholog(s) of this gene implicated in asthma; chronic obstructive pulmonary disease; immunodeficiency 29; and salmonellosis. Orthologous to human IL12B (interleukin 12B).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


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