Mouse IL-12/IL-23p40 Standard (小鼠 IL-12/IL-23p40 标准品)
¥280.00
- 分子靶点:IL12, IL12B, NKSF, CLMF2
- 种属:小鼠 (Mouse)
- 试剂盒:EK2183
- 保存:短期4℃保存,长期-20℃保存
- 运输条件:4℃蓝冰运输
在售SKU:70-EK2183S
描述
文章目录[隐藏]
本产品只包含标准品试剂,如需购买试剂盒请点击下图
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- EK2183
- ELISA试剂盒
Mouse IL-12/IL-23p40 ELISA Kit检测试剂盒(酶联免疫吸附法)
- ¥1,600.00 – ¥2,650.00
商品名 |
Mouse IL-12/IL-23p40 Standard (小鼠 IL-12/IL-23p40 标准品) |
---|---|
组分 |
小鼠IL-12/IL-23p40标准品 |
检测方法 |
双抗夹心法 |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
板式 |
管 |
保存 |
短期4℃,长期-20℃保存 |
运输条件 |
4℃蓝冰运输 |
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-12/IL-23p40抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-12/IL-23p40与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-12/IL-23p40的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
IL12B 分子靶点信息概述
- 分子名:IL12B, interleukin 12B
- 基因家族:Immunoglobulin like domain containing; Interleukins
- 别名:CLMF; IL-12B; NKSF; CLMF2
- 曾用名:NKSF2
- 全称:natural killer cell stimulatory factor-2; cytotoxic lymphocyte maturation factor 2, p40; interleukin 12, p40; natural killer cell stimulatory factor, 40 kD subunit; interleukin-12 beta chain; IL12, subunit p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
IL12B 分子靶点综述
白细胞介素12 (IL-12)由树突状细胞、巨噬细胞、中性粒细胞和人B淋巴母细胞株 (NC-37)在响应抗原刺激时自然产生。IL-12由4个а螺旋组成,是一个由2种独立基因--- IL-12A (p35)和IL-12B (p40)编码形成的异源二聚体细胞因子。蛋白合成之后可形成有生物活性的异源二聚体(p70)和同源二聚体p40。IL-12参与初始T细胞向Th1细胞分化的过程,作为T细胞刺激因子,它可刺激T细胞的生长及其功能发挥。IL-12具有抗血管生成活性,对自然杀伤细胞和T淋巴细胞活性有重要作用。同样地,IL-12与自身免疫相关。
小鼠 Mouse Il12b 分子靶点信息
- 分子名:Il12b, interleukin 12b
- 别称:
- IL-12 p40
- Il-12b
- Il-12p40
- IL-23 subunit p40
- 基因序列:NCBI_Gene: 16160
- 蛋白序列:
小鼠 Mouse Il12b 靶点分子功能(预测)
Enables identical protein binding activity and interleukin-12 alpha subunit binding activity. Contributes to cytokine activity and cytokine receptor binding activity. Involved in positive regulation of T cell mediated cytotoxicity; positive regulation of T-helper 1 type immune response; and positive regulation of defense response to virus by host. Acts upstream of or within several processes, including cellular response to lipopolysaccharide; defense response to other organism; and positive regulation of interferon-gamma production. Located in cell surface; cytoplasm; and extracellular space. Part of interleukin-12 complex and interleukin-23 complex. Is expressed in colon. Human ortholog(s) of this gene implicated in asthma; chronic obstructive pulmonary disease; immunodeficiency 29; and salmonellosis. Orthologous to human IL12B (interleukin 12B).
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
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- ELISA通关必备丨样本篇丨不常见样本 
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- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
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- 查看更多ELISA操作相关问题
ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- Gastrodin Attenuates Lipopolysaccharide-Induced Inflammatory Response and Migration via the Notch-1 Signaling Pathway in Activated Microglia 
- Comparative studies of macrophage-biased responses in mice to infection with Toxoplasma gondii ToxoDB #9 strains of different virulence isolated from China 
- Microneedle-assisted genome editing: A transdermal strategy of targeting NLRP3 by CRISPR-Cas9 for synergistic therapy of inflammatory skin disorders 
- On-demand integrated nano-engager converting cold tumors to hot via increased DNA damage and dual immune checkpoint inhibition 
- Original research: Synergy between Toxoplasma gondii type I ΔGRA17 immunotherapy and PD-L1 checkpoint inhibition triggers the regression of targeted and distal tumors 
- GTS-21 Reduces Inflammation in Acute Lung Injury by Regulating M1 Polarization and Function of Alveolar Macrophages 
- A therapeutic DC vaccine with maintained immunological activity exhibits robust anti-tumor efficacy 
- A uracil auxotroph Toxoplasma gondii exerting immunomodulation to inhibit breast cancer growth and metastasis 
- Acupuncture Needling, Electroacupuncture, and Fire Needling Improve Imiquimod-Induced Psoriasis-Like Skin Lesions through Reducing Local Inflammatory Responses 
- TL1A primed dendritic cells activation exacerbated chronic murine colitis 
- Immunometabolism-modulation and immunotoxicity evaluation of perfluorooctanoic acid in macrophage 
- Peucedanum praeruptorum Dunn polysaccharides regulate macrophage inflammatory response through TLR2/TLR4-mediated MAPK and NF-κB pathways 
- ANG II facilitated CD11+Ly6Chi cells reprogramming into M1-like macrophage through Erk1/2 or p38-Stat3 pathway and involved in EAM 
- Toxoplasma gondii bradyzoite-specific BAG1 is nonessential for cyst formation due to compensation by other heat-shock proteins 
- A polymeric nanoplatform enhances the cGAS-STING pathway in macrophages to potentiate phagocytosis for cancer immunotherapy 
- Dual-Functional Nanodroplet for Tumor Vasculature Ultrasound Imaging and Tumor Immunosuppressive Microenvironment Remodeling 
- Boron Dipyrromethene-Based Nanotheranostic System for Sonophotoassisted Therapy and Simultaneous Monitoring of Tumor Immune Microenvironment Reprogramming 
- Paroxetine alleviates dendritic cell and T lymphocyte activation via GRK2-mediated PI3K–AKT signaling in rheumatoid arthritis 
- Structural characteristics and immunomodulation activity of a polysaccharide from purslane (Portulaca oleracea) 
- Efficient TNBC immunotherapy by dual reprogramming tumor-infiltrating dendritic cells and tumor-associated macrophages with stimulus-responsive miR155 nanocomplexes 
- Toxoplasma gondii gra5 deletion mutant protects hosts against Toxoplasma gondii infection and breast tumors 
- Surface Functional Modification by Ti3C2Tx MXene on PLLA Nanofibers for Optimizing Neural Stem Cell Engineering 
- An injectable signal-amplifying device elicits a specific immune response against malignant glioblastoma 
- Regulating T-cell metabolic reprogramming and blocking PD-1 co-promote personalized postoperative autologous nanovaccines 
- Adjuvant Efficacy of the ECMS-Oil on Immune Responses against Bordetella bronchiseptica in Mice through the TLR2/MyD88/NF-κB Pathway 
- Dissolving Microneedle Arrays as a Hepatitis B Vaccine Delivery System Adjuvanted by APC-Targeted Poly (Lactic-co-Glycolic Acid) (PLGA) Nanoparticles 
- In vitro and in vivo exploration of the anti-atopic dermatitis mechanism of action of Tibetan medicine Qi-Sai-Er-Sang-Dang-Song decoction 
- A polymeric nanoplatform enhances the cGAS-STING pathway in macrophages to potentiate phagocytosis for cancer immunotherapy 
- A polymeric nanoplatform enhances the cGAS-STING pathway in macrophages to potentiate phagocytosis for cancer immunotherapy 
- A therapeutic DC vaccine with maintained immunological activity exhibits robust anti-tumor efficacy 
- Critical roles of conventional dendritic cells in autoimmune hepatitis via autophagy regulation 
Gastrodin Attenuates Lipopolysaccharide-Induced Inflammatory Response and Migration via the Notch-1 Signaling Pathway in Activated Microglia 
Comparative studies of macrophage-biased responses in mice to infection with Toxoplasma gondii ToxoDB #9 strains of different virulence isolated from China 
Microneedle-assisted genome editing: A transdermal strategy of targeting NLRP3 by CRISPR-Cas9 for synergistic therapy of inflammatory skin disorders 
On-demand integrated nano-engager converting cold tumors to hot via increased DNA damage and dual immune checkpoint inhibition 
Original research: Synergy between Toxoplasma gondii type I ΔGRA17 immunotherapy and PD-L1 checkpoint inhibition triggers the regression of targeted and distal tumors 
GTS-21 Reduces Inflammation in Acute Lung Injury by Regulating M1 Polarization and Function of Alveolar Macrophages 
A therapeutic DC vaccine with maintained immunological activity exhibits robust anti-tumor efficacy 
A uracil auxotroph Toxoplasma gondii exerting immunomodulation to inhibit breast cancer growth and metastasis 
Acupuncture Needling, Electroacupuncture, and Fire Needling Improve Imiquimod-Induced Psoriasis-Like Skin Lesions through Reducing Local Inflammatory Responses 
TL1A primed dendritic cells activation exacerbated chronic murine colitis 
Immunometabolism-modulation and immunotoxicity evaluation of perfluorooctanoic acid in macrophage 
Peucedanum praeruptorum Dunn polysaccharides regulate macrophage inflammatory response through TLR2/TLR4-mediated MAPK and NF-κB pathways 
ANG II facilitated CD11+Ly6Chi cells reprogramming into M1-like macrophage through Erk1/2 or p38-Stat3 pathway and involved in EAM 
Toxoplasma gondii bradyzoite-specific BAG1 is nonessential for cyst formation due to compensation by other heat-shock proteins 
A polymeric nanoplatform enhances the cGAS-STING pathway in macrophages to potentiate phagocytosis for cancer immunotherapy 
Dual-Functional Nanodroplet for Tumor Vasculature Ultrasound Imaging and Tumor Immunosuppressive Microenvironment Remodeling 
Boron Dipyrromethene-Based Nanotheranostic System for Sonophotoassisted Therapy and Simultaneous Monitoring of Tumor Immune Microenvironment Reprogramming 
Paroxetine alleviates dendritic cell and T lymphocyte activation via GRK2-mediated PI3K–AKT signaling in rheumatoid arthritis 
Structural characteristics and immunomodulation activity of a polysaccharide from purslane (Portulaca oleracea) 
Efficient TNBC immunotherapy by dual reprogramming tumor-infiltrating dendritic cells and tumor-associated macrophages with stimulus-responsive miR155 nanocomplexes 
Toxoplasma gondii gra5 deletion mutant protects hosts against Toxoplasma gondii infection and breast tumors 
Surface Functional Modification by Ti3C2Tx MXene on PLLA Nanofibers for Optimizing Neural Stem Cell Engineering 
An injectable signal-amplifying device elicits a specific immune response against malignant glioblastoma 
Regulating T-cell metabolic reprogramming and blocking PD-1 co-promote personalized postoperative autologous nanovaccines 
Adjuvant Efficacy of the ECMS-Oil on Immune Responses against Bordetella bronchiseptica in Mice through the TLR2/MyD88/NF-κB Pathway 
Dissolving Microneedle Arrays as a Hepatitis B Vaccine Delivery System Adjuvanted by APC-Targeted Poly (Lactic-co-Glycolic Acid) (PLGA) Nanoparticles 
In vitro and in vivo exploration of the anti-atopic dermatitis mechanism of action of Tibetan medicine Qi-Sai-Er-Sang-Dang-Song decoction 
A polymeric nanoplatform enhances the cGAS-STING pathway in macrophages to potentiate phagocytosis for cancer immunotherapy 
A polymeric nanoplatform enhances the cGAS-STING pathway in macrophages to potentiate phagocytosis for cancer immunotherapy 
- 组织匀浆 组织细胞 血清
A therapeutic DC vaccine with maintained immunological activity exhibits robust anti-tumor efficacy 
- 细胞培养上清 血浆
Critical roles of conventional dendritic cells in autoimmune hepatitis via autophagy regulation 
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