EK2183

Mouse IL-12/IL-23p40 ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：IL12, IL12B, NKSF, CLMF2
  • 种属：小鼠 (Mouse)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：20 μL
  • 灵敏度：0.25 pg/mL
  • 检测范围：4.69 pg/mL - 300 pg/mL
  • 回收率：85% - 112%

在售SKU:70-EK2183/2-48, 70-EK2183/2-96

描述

商品名

Mouse IL-12/IL-23p40 ELISA Kit (小鼠 IL-12/IL-23p40 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：2.3% - 3.0%；板间变异系数：3.6% - 6.2%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

20 μL
灵敏度

0.25 pg/mL
检测范围

4.69 pg/mL - 300 pg/mL
回收率

85% - 112%
平均回收率

0.98
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IL-12/IL-23p40单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠IL-12/IL-23p40冻干标准品
  • IL-12/IL-23p40检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠IL-12/IL-23p40抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-12/IL-23p40与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-12/IL-23p40的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse IL-12/IL-23p40 ELISA Kit (小鼠 IL-12/IL-23p40 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 IL12B, NKSF, CLMF2 靶点信息

IL12B 分子靶点信息概述

  • 分子名：IL12B, interleukin 12B
  • 基因家族：Immunoglobulin like domain containing; Interleukins
  • 别名：CLMF; IL-12B; NKSF; CLMF2
  • 曾用名：NKSF2
  • 全称：natural killer cell stimulatory factor-2; cytotoxic lymphocyte maturation factor 2, p40; interleukin 12, p40; natural killer cell stimulatory factor, 40 kD subunit; interleukin-12 beta chain; IL12, subunit p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)

IL12B 分子靶点综述

白细胞介素12 (IL-12)由树突状细胞、巨噬细胞、中性粒细胞和人B淋巴母细胞株 (NC-37)在响应抗原刺激时自然产生。IL-12由4个а螺旋组成，是一个由2种独立基因--- IL-12A (p35)和IL-12B (p40)编码形成的异源二聚体细胞因子。蛋白合成之后可形成有生物活性的异源二聚体(p70)和同源二聚体p40。IL-12参与初始T细胞向Th1细胞分化的过程，作为T细胞刺激因子，它可刺激T细胞的生长及其功能发挥。IL-12具有抗血管生成活性，对自然杀伤细胞和T淋巴细胞活性有重要作用。同样地，IL-12与自身免疫相关。

小鼠 Mouse Il12b 分子靶点信息

小鼠 Mouse Il12b 靶点分子功能（预测）

Enables identical protein binding activity and interleukin-12 alpha subunit binding activity. Contributes to cytokine activity and cytokine receptor binding activity. Involved in positive regulation of T cell mediated cytotoxicity; positive regulation of T-helper 1 type immune response; and positive regulation of defense response to virus by host. Acts upstream of or within several processes, including cellular response to lipopolysaccharide; defense response to other organism; and positive regulation of interferon-gamma production. Located in cell surface; cytoplasm; and extracellular space. Part of interleukin-12 complex and interleukin-23 complex. Is expressed in colon. Human ortholog(s) of this gene implicated in asthma; chronic obstructive pulmonary disease; immunodeficiency 29; and salmonellosis. Orthologous to human IL12B (interleukin 12B).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Gastrodin Attenuates Lipopolysaccharide-Induced Inflammatory Response and Migration via the Notch-1 Signaling Pathway in Activated Microglia 

  2. Comparative studies of macrophage-biased responses in mice to infection with Toxoplasma gondii ToxoDB #9 strains of different virulence isolated from China 

  3. Microneedle-assisted genome editing: A transdermal strategy of targeting NLRP3 by CRISPR-Cas9 for synergistic therapy of inflammatory skin disorders 

  4. On-demand integrated nano-engager converting cold tumors to hot via increased DNA damage and dual immune checkpoint inhibition 

  5. Original research: Synergy between Toxoplasma gondii type I ΔGRA17 immunotherapy and PD-L1 checkpoint inhibition triggers the regression of targeted and distal tumors 

  6. GTS-21 Reduces Inflammation in Acute Lung Injury by Regulating M1 Polarization and Function of Alveolar Macrophages 

  7. A therapeutic DC vaccine with maintained immunological activity exhibits robust anti-tumor efficacy 

  8. A uracil auxotroph Toxoplasma gondii exerting immunomodulation to inhibit breast cancer growth and metastasis 

  9. Acupuncture Needling, Electroacupuncture, and Fire Needling Improve Imiquimod-Induced Psoriasis-Like Skin Lesions through Reducing Local Inflammatory Responses 

  10. TL1A primed dendritic cells activation exacerbated chronic murine colitis 

  11. Immunometabolism-modulation and immunotoxicity evaluation of perfluorooctanoic acid in macrophage 

  12. Peucedanum praeruptorum Dunn polysaccharides regulate macrophage inflammatory response through TLR2/TLR4-mediated MAPK and NF-κB pathways 

  13. ANG II facilitated CD11+Ly6Chi cells reprogramming into M1-like macrophage through Erk1/2 or p38-Stat3 pathway and involved in EAM 

  14. Toxoplasma gondii bradyzoite-specific BAG1 is nonessential for cyst formation due to compensation by other heat-shock proteins 

  15. A polymeric nanoplatform enhances the cGAS-STING pathway in macrophages to potentiate phagocytosis for cancer immunotherapy 

  16. Dual-Functional Nanodroplet for Tumor Vasculature Ultrasound Imaging and Tumor Immunosuppressive Microenvironment Remodeling 

  17. Boron Dipyrromethene-Based Nanotheranostic System for Sonophotoassisted Therapy and Simultaneous Monitoring of Tumor Immune Microenvironment Reprogramming 

  18. Paroxetine alleviates dendritic cell and T lymphocyte activation via GRK2-mediated PI3K–AKT signaling in rheumatoid arthritis 

  19. Structural characteristics and immunomodulation activity of a polysaccharide from purslane (Portulaca oleracea) 

  20. Efficient TNBC immunotherapy by dual reprogramming tumor-infiltrating dendritic cells and tumor-associated macrophages with stimulus-responsive miR155 nanocomplexes 

  21. Toxoplasma gondii gra5 deletion mutant protects hosts against Toxoplasma gondii infection and breast tumors 

  22. Surface Functional Modification by Ti3C2Tx MXene on PLLA Nanofibers for Optimizing Neural Stem Cell Engineering 

  23. An injectable signal-amplifying device elicits a specific immune response against malignant glioblastoma 

  24. Regulating T-cell metabolic reprogramming and blocking PD-1 co-promote personalized postoperative autologous nanovaccines 

  25. Adjuvant Efficacy of the ECMS-Oil on Immune Responses against Bordetella bronchiseptica in Mice through the TLR2/MyD88/NF-κB Pathway 

  26. Dissolving Microneedle Arrays as a Hepatitis B Vaccine Delivery System Adjuvanted by APC-Targeted Poly (Lactic-co-Glycolic Acid) (PLGA) Nanoparticles 

  27. In vitro and in vivo exploration of the anti-atopic dermatitis mechanism of action of Tibetan medicine Qi-Sai-Er-Sang-Dang-Song decoction 

  28. A polymeric nanoplatform enhances the cGAS-STING pathway in macrophages to potentiate phagocytosis for cancer immunotherapy 

  29. A polymeric nanoplatform enhances the cGAS-STING pathway in macrophages to potentiate phagocytosis for cancer immunotherapy 

  30. 组织匀浆 组织细胞 血清

    A therapeutic DC vaccine with maintained immunological activity exhibits robust anti-tumor efficacy 

  31. 细胞培养上清 血浆

    Critical roles of conventional dendritic cells in autoimmune hepatitis via autophagy regulation 

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