EK2173

Mouse Granzyme B ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：GZMB, granzyme B, CTLA1
  • 种属：小鼠 (Mouse)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：10 μL
  • 灵敏度：0.91 pg/mL
  • 检测范围：0.31 ng/mL - 20 ng/mL
  • 回收率：91% - 105%

在售SKU:70-EK2173-48, 70-EK2173-96

描述

商品名

Mouse Granzyme B ELISA Kit (小鼠颗粒酶B ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：2.9%-3.9%；板间变异系数：1.8% - 2.8%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

10 μL
灵敏度

0.91 pg/mL
检测范围

0.31 ng/mL - 20 ng/mL
回收率

91% - 105%
平均回收率

0.99
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗Granzyme B单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠Granzyme B冻干标准品
  • Granzyme B检测抗体
  • 辣根过氧化物酶标记的链霉亲和素
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠 Granzyme B 抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的 Granzyme B 与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中 Granzyme B 的浓度成正比。加入终止液终止反应，在 450 nm 波长(参考波长 570 - 630 nm)测定吸光度值。

Mouse Granzyme B ELISA Kit (小鼠颗粒酶B ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 GZMB, granzyme B, CTLA1 靶点信息

GZMB 分子靶点信息概述

  • 分子名：GZMB, granzyme B
  • 基因家族：Granule associated serine proteases of immune defence
  • 别名：CCPI; CGL-1; CSP-B; CGL1; CTSGL1; HLP; SECT
  • 曾用名：CTLA1; CSPB
  • 全称：fragmentin 2; cytotoxic serine protease B; cathepsin G-like 1; T-cell serine protease 1-3E; granzyme 2; cytotoxic T-lymphocyte-associated serine esterase 1; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)

GZMB 分子靶点综述

颗粒酶B(GranzymeB)是一种丝氨酸蛋白酶，最常见于细胞毒性淋巴细胞(CTLs)和自然杀伤细胞(NK细胞)颗粒中。由这些细胞分泌的GranzymeB和穿孔蛋白一起作用介导靶细胞的凋亡。最近，人们发现许多非细胞毒性细胞从嗜碱性粒细胞、肥大细胞到平滑肌细胞也可产生GranzymeB。GranzymeB通过刺激细胞因子的释放参与诱导炎症，同样也参与细胞外基质重塑。升高的GranzymeB水平与许多自身免疫性疾病、一些皮肤病和1型糖尿病有关。

小鼠 Mouse Gzme 分子靶点信息

  • 分子名：Gzme, granzyme E
  • 别称：
    • CCP3
    • Ctla-6
    • Ctla6
    • cytotoxic T lymphocyte-associated protein 6
    • MCSP-2
  • 基因序列：NCBI_Gene: 14942
  • 蛋白序列：

小鼠 Mouse Gzme 靶点分子功能（预测）

Predicted to enable serine-type endopeptidase activity. Predicted to be involved in several processes, including granzyme-mediated programmed cell death signaling pathway; natural killer cell mediated cytotoxicity; and pyroptosis. Predicted to act upstream of or within cytolysis. Predicted to be located in cytolytic granule. Predicted to be active in cytoplasm and intracellular membrane-bounded organelle. Is expressed in extraembryonic component; placenta; spongiotrophoblast; and trophoblast giant cell. Orthologous to human GZMB (granzyme B).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Dual Inhibition of Endoplasmic Reticulum Stress and Oxidation Stress Manipulates the Polarization of Macrophages under Hypoxia to Sensitize Immunotherapy 

  2. METTL3-mediated m6A RNA methylation promotes the anti-tumour immunity of natural killer cells 

  3. Administration Routes of Polyethylenimine-Coated PLGA Nanoparticles Encapsulating Angelica Sinensis Polysaccharide Vaccine Delivery System Affect Immune Responses 

  4. Antitumor efficacy of chimeric antigen receptor T cells against EGFRvIII-expressing glioblastoma in C57BL/6 mice 

  5. Intravenous injection of the oncolytic virus M1 awakens antitumor T cells and overcomes resistance to checkpoint blockade 

  6. A Vaccination with Boosted Cross Presentation by ER-Targeted Antigen Delivery for Anti-Tumor Immunotherapy 

  7. Olaparib Suppresses MDSC Recruitment via SDF1α/CXCR4 Axis to Improve the Anti-tumor Efficacy of CAR-T Cells on Breast Cancer in Mice 

  8. Tumor-derived Jagged1 promotes cancer progression through immune evasion 

  9. CXCL10-armed oncolytic adenovirus promotes tumor-infiltrating T-cell chemotaxis to enhance anti-PD-1 therapy 

  10. Cytotoxic T lymphocyte-associated protein 4 antibody aggrandizes antitumor immune response of oncolytic virus M1 via targeting regulatory T cells 

  11. Pickering emulsion stabilized by Chinese Yam polysaccharides PLGA for enhanced humoral and cellular immune responses 

  12. Liver type 1 innate lymphoid cells develop locally via an interferon-γ–dependent loop 

  13. Lycium barbarum polysaccharides-loaded Particulate Alum via Pickering emulsion as an adjuvant to enhance immune responses 

  14. Coexpression of IL7 and CCL21 Increases Efficacy of CAR-T Cells in Solid Tumors without Requiring Preconditioned Lymphodepletion 

  15. Resveratrol ameliorates Lewis lung carcinoma-bearing mice development, decreases granulocytic myeloid-derived suppressor cell accumulation and impairs its suppressive ability 

  16. Inhibition of PCSK9 enhances the antitumor effect of PD-1 inhibitor in colorectal cancer by promoting the infiltration of CD8+ T cells and the exclusion of Treg cells 

  17. IL-1β mediates Candida tropicalis-induced immunosuppressive function of MDSCs to foster colorectal cancer 

  18. Controlling the speed of antigens transport in dendritic cells improves humoral and cellular immunity for vaccine 

  19. Astragalus polysaccharides promote neural stem cells-derived oligodendrogenesis through attenuating CD8+T cell infiltration in experimental autoimmune encephalomyelitis 

  20. An mRNA-based broad-spectrum vaccine candidate confers cross-protection against heterosubtypic influenza A viruses 

  21. Deubiquitinase inhibitor PR-619 potentiates colon cancer immunotherapy by inducing ferroptosis 

  22. An inflammatory checkpoint generated by IL1RN splicing offers therapeutic opportunity for KRAS mutant intrahepatic cholangiocarcinoma 

  23. Damage-induced NAD release activates intestinal CD4+ and CD8+ T cell via P2X7R signaling 

  24. 细胞培养上清

    Cytotoxic T Lymphocyte-Associated Protein 4 Antibody Aggrandizes Antitumor Immune Response of Oncolytic Virus M1 Via Targeting Regulatory T Cells 

  25. 细胞培养上清

    Intravenous Injection of the Oncolytic Virus M1 Awakens Antitumor T Cells and Overcomes Resistance to Checkpoint Blockade 

  26. 组织匀浆 脾脏 血清

    Administration Routes of Polyethylenimine-Coated Plga Nanoparticles Encapsulating Angelica Sinensis Polysaccharide Vaccine Delivery System Affect Immune Responses 

  27. 细胞培养上清

    Overcoming resistance to oncolytic virus M1 by targeting PI3K-γ in tumor-associated myeloid cells 

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