Mouse Fas/TNFRSF6/CD95 Standard (小鼠Fas 标准品)
¥280.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:FAS, TNFRSF6, CD95, APO-1
- 种属:小鼠 (Mouse)
- 试剂盒:EK2F01
- 保存:短期4℃保存,长期-20℃保存
- 运输条件:4℃蓝冰运输
在售SKU:70-EK2F01S
描述
文章目录[隐藏]
本产品只包含标准品试剂,如需购买试剂盒请点击下图
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- EK2F01
- ELISA试剂盒
Mouse Fas/TNFRSF6/CD95 ELISA Kit检测试剂盒(酶联免疫吸附法)
- ¥2,000.00 – ¥3,400.00
商品名 |
Mouse Fas/TNFRSF6/CD95 Standard (小鼠Fas 标准品) |
---|---|
组分 |
小鼠Fas冻干标准品 |
检测方法 |
双抗夹心法 |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
板式 |
管 |
保存 |
短期4℃,长期-20℃保存 |
运输条件 |
4℃蓝冰运输 |
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠Fas抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的Fas与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中Fas的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
FAS 分子靶点信息概述
- 分子名:FAS, Fas cell surface death receptor
- 基因家族:CD molecules; Tumor necrosis factor receptor superfamily; Death inducing signaling complex
- 别名:CD95; APO-1
- 曾用名:FAS1; APT1; TNFRSF6
- 全称:TNF receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6; Fas (TNF receptor superfamily, member 6)
FAS 分子靶点综述
Fas,又名APO-1、CD95或TNFRSF6,是一个可转导凋亡信号的细胞表面受体。它是两种凋亡途径之一,另一种是线粒体途径。一旦结合配体,Fas就会形成死亡诱导信号复合物。相邻细胞表面的膜锚定Fas配体三聚体会导致Fas低聚化。近来的小鼠模型研究表明,Fas促进其肿瘤生长,但是人类癌症基因组学数据库表明Fas可作为肿瘤抑制物。在AOM-DSS诱导的结肠癌和MCA诱导的肉瘤小鼠模型中,结果显示Fas是肿瘤抑制物。另外,Fas还可介导肿瘤特异性细胞毒性T细胞的抗肿瘤细胞毒作用。研究报道,癌症和自身免疫性疾病患者的血清Fas水平是升高的,前者可能来源于肿瘤细胞本身。
小鼠 Mouse Fas 分子靶点信息
- 分子名:Fas, Fas (TNF receptor superfamily member 6)
- 别称:
- AI196731
- APO-1
- CD95
- expressed sequence AI196731
- lpr
- lymphoproliferation
- TNF receptor superfamily member 6
- TNFR6
- Tnfrsf6
- 基因序列:NCBI_Gene: 14102
- 蛋白序列:
小鼠 Mouse Fas 靶点分子功能(预测)
Enables identical protein binding activity. Involved in positive regulation of protein-containing complex assembly. Acts upstream of or within several processes, including extrinsic apoptotic signaling pathway; hematopoietic or lymphoid organ development; and regulation of hemopoiesis. Located in external side of plasma membrane and extracellular region. Part of CD95 death-inducing signaling complex. Is expressed in several structures, including alimentary system; brain; genitourinary system; hemolymphoid system gland; and liver and biliary system. Used to study Sjogren's syndrome; autoimmune lymphoproliferative syndrome; systemic lupus erythematosus; and type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); cystic fibrosis; hematologic cancer (multiple); pre-eclampsia (multiple); and urinary system cancer (multiple). Orthologous to human FAS (Fas cell surface death receptor).
操作步骤
文章目录[隐藏]
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- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
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ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
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- A ginseng polysaccharide protects intestinal barrier integrity in high-fat diet-fed obese mice 
Effects of sulfated polysaccharides from Laminaria japonica on regularating the gut microbiotan and alleviating intestinal inflammation in obese mice 
A ginseng polysaccharide protects intestinal barrier integrity in high-fat diet-fed obese mice 
A ginseng polysaccharide protects intestinal barrier integrity in high-fat diet-fed obese mice 
A ginseng polysaccharide protects intestinal barrier integrity in high-fat diet-fed obese mice 
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