EK2F01S

Mouse Fas/TNFRSF6/CD95 Standard (小鼠Fas 标准品)

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¥280.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:FAS, TNFRSF6, CD95, APO-1
  • 种属:小鼠 (Mouse)
  • 试剂盒:EK2F01
  • 保存:短期4℃保存,长期-20℃保存
  • 运输条件:4℃蓝冰运输

在售SKU:70-EK2F01S


描述

本产品只包含标准品试剂,如需购买试剂盒请点击下图

商品名

Mouse Fas/TNFRSF6/CD95 Standard (小鼠Fas 标准品)

组分

小鼠Fas冻干标准品

检测方法

双抗夹心法

样本类型

血清,血浆,细胞培养上清及其他生物学样本

板式

保存

短期4℃,长期-20℃保存

运输条件

4℃蓝冰运输

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠Fas抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的Fas与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中Fas的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse Fas/TNFRSF6/CD95 ELISA Kit (小鼠Fas ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 FAS, TNFRSF6, CD95, APO-1 靶点信息

FAS 分子靶点信息概述

  • 分子名:FAS, Fas cell surface death receptor
  • 基因家族:CD molecules; Tumor necrosis factor receptor superfamily; Death inducing signaling complex
  • 别名:CD95; APO-1
  • 曾用名:FAS1; APT1; TNFRSF6
  • 全称:TNF receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6; Fas (TNF receptor superfamily, member 6)

FAS 分子靶点综述

Fas,又名APO-1、CD95或TNFRSF6,是一个可转导凋亡信号的细胞表面受体。它是两种凋亡途径之一,另一种是线粒体途径。一旦结合配体,Fas就会形成死亡诱导信号复合物。相邻细胞表面的膜锚定Fas配体三聚体会导致Fas低聚化。近来的小鼠模型研究表明,Fas促进其肿瘤生长,但是人类癌症基因组学数据库表明Fas可作为肿瘤抑制物。在AOM-DSS诱导的结肠癌和MCA诱导的肉瘤小鼠模型中,结果显示Fas是肿瘤抑制物。另外,Fas还可介导肿瘤特异性细胞毒性T细胞的抗肿瘤细胞毒作用。研究报道,癌症和自身免疫性疾病患者的血清Fas水平是升高的,前者可能来源于肿瘤细胞本身。

小鼠 Mouse Fas 分子靶点信息

小鼠 Mouse Fas 靶点分子功能(预测)

Enables identical protein binding activity. Involved in positive regulation of protein-containing complex assembly. Acts upstream of or within several processes, including extrinsic apoptotic signaling pathway; hematopoietic or lymphoid organ development; and regulation of hemopoiesis. Located in external side of plasma membrane and extracellular region. Part of CD95 death-inducing signaling complex. Is expressed in several structures, including alimentary system; brain; genitourinary system; hemolymphoid system gland; and liver and biliary system. Used to study Sjogren's syndrome; autoimmune lymphoproliferative syndrome; systemic lupus erythematosus; and type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); cystic fibrosis; hematologic cancer (multiple); pre-eclampsia (multiple); and urinary system cancer (multiple). Orthologous to human FAS (Fas cell surface death receptor).

操作步骤

ELISA操作常见问题

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引用文献


  1. Effects of sulfated polysaccharides from Laminaria japonica on regularating the gut microbiotan and alleviating intestinal inflammation in obese mice 

  2. A ginseng polysaccharide protects intestinal barrier integrity in high-fat diet-fed obese mice 

  3. A ginseng polysaccharide protects intestinal barrier integrity in high-fat diet-fed obese mice 

  4. A ginseng polysaccharide protects intestinal barrier integrity in high-fat diet-fed obese mice 

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