EK2143

Mouse CXCL9/MIG ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:CXCL9, MIG, SCYB9
  • 种属:小鼠 (Mouse)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:20 μL
  • 灵敏度:1.82 pg/mL
  • 检测范围:15.63 pg/mL - 1000 pg/mL
  • 回收率:78% - 120%

在售SKU:70-EK2143/2-48, 70-EK2143/2-96


描述

商品名

Mouse CXCL9/MIG ELISA Kit (小鼠趋化因子CXC配体9 (CXCL9) ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.1% - 4.5%;板间变异系数:3.8% - 6.8%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

20 μL

灵敏度

1.82 pg/mL

检测范围

15.63 pg/mL - 1000 pg/mL

回收率

78% - 120%

平均回收率

0.98

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗CXCL9/MIG单克隆抗体的96孔聚苯乙烯酶标板
  • 小鼠CXCL9/MIG冻干标准品
  • CXCL9/MIG检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠CXCL9抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的CXCL9与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中CXCL9的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse CXCL9/MIG ELISA Kit (小鼠趋化因子CXC配体9 (CXCL9) ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 CXCL9, MIG, SCYB9 靶点信息

CXCL9 分子靶点信息概述

  • 分子名:CXCL9, C-X-C motif chemokine ligand 9
  • 基因家族:Chemokine ligands
  • 别名:SCYB9; Humig; crg-10
  • 曾用名:CMK; MIG
  • 全称:monokine induced by gamma interferon; chemokine (C-X-C motif) ligand 9

CXCL9 分子靶点综述

趋化因子CXC配体9(CXCL9),又名γ干扰素诱导的单核因子(MIG),是属于CXC趋化因子家族的一个小分子量细胞因子。它与其它两个CXC趋化因子CXCL10和CXCL11密切相关,它们都是与趋化因子受体CXCR3相互作用来发挥其趋化功能。CXCL9参与Th1型炎症,它有许多功能,主要的功能是化学吸引。活化的B细胞、单核细胞、CD8+记忆性T细胞和CD4+Th1T细胞都可对CXCL9作出应答。CXCL9还可通过作为IL-4/Th2应答的拮抗剂来促进Th1应答,这是通过阻断Th2相关趋化因子(eotaxin)发生的,从而限制Th2的浸润。CXCL9还可通过其延伸的C端展现出直接的抗微生物活性。在造血中,CXCL9参与CD34+干细胞的动员和迁移、抑制红系和髓系细胞集落的形成。

小鼠 Mouse Cxcl9 分子靶点信息

小鼠 Mouse Cxcl9 靶点分子功能(预测)

Predicted to enable CXCR3 chemokine receptor binding activity and chemokine activity. Acts upstream of or within several processes, including defense response to virus; positive regulation of myoblast differentiation; and positive regulation of myoblast fusion. Located in external side of plasma membrane and extracellular space. Is expressed in central nervous system; retina; stomach; testis; and thymus. Orthologous to human CXCL9 (C-X-C motif chemokine ligand 9).

操作步骤

ELISA操作常见问题

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Antitumor Effect and Immune Response of Nanosecond Pulsed Electric Fields in Pancreatic Cancer 

  2. MiR-19a-3p Suppresses M1 Macrophage Polarization by Inhibiting STAT1/IRF1 Pathway 

  3. Cocktail strategy for ‘cold’ tumors therapy via active recruitment of CD8+ T cells and enhancing their function 

  4. Isoforskolin Alleviates AECOPD by Improving Pulmonary Function and Attenuating Inflammation Which Involves Downregulation of Th17/IL-17A and NF-κB/NLRP3 

  5. Combination of Fruquintinib and Anti–PD-1 for the Treatment of Colorectal Cancer 

  6. Boosting Tumor Immunotherapy by Bioactive Nanoparticles via Ca2+ Interference Mediated TME Reprogramming and Specific PD-L1 Depletion 

  7. Lentinan inhibits tumor angiogenesis via interferon γ and in a T cell independent manner 

  8. Itaconate inhibits TET DNA dioxygenases to dampen inflammatory responses 

  9. Reversing EZH2-Mediated Epigenetic Silence with Photodynamic Polymeric Nanoparticles for Synergistically Enhanced Antitumor Immunotherapy 

  10. Psoralen attenuates cigarette smoke extract-induced inflammation by modulating CD8+ T lymphocyte recruitment and chemokines via the JAK2/STAT1 signaling pathway 

  11. Chemical conjugation mitigates immunotoxicity of chemotherapy via reducing receptor-mediated drug leakage from lipid nanoparticles 

  12. Targeting BCL9/BCL9L enhances antigen presentation by promoting conventional type 1 dendritic cell (cDC1) activation and tumor infiltration 

  13. HIF-1α Mediates Immunosuppression and Chemoresistance in Colorectal Cancer by Inhibiting CXCL9, ?10 and ?11 

  14. Immunostimulant Hydrogel-Guided Tumor Microenvironment Reprogramming to Efficiently Potentiate Macrophage-Mediated Cellular Phagocytosis for Systemic Cancer Immunotherapy 

  15. Enhancement of Microglia Functions by Developed Nano-Immuno-Synergist to Ameliorate Immunodeficiency for Malignant Glioma Treatment 

  16. 组织匀浆 肿瘤

    Cocktail Strategy for 'Cold' Tumors Therapy Via Active Recruitment of Cd8+ T Cells and Enhancing Their Function 

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