Mouse CXCL1/KC ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:CXCL1, SCYB1, NAP-3
- 种属:小鼠 (Mouse)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:20 μL
- 灵敏度:0.47 pg/mL
- 检测范围:31.25 pg/mL - 2000 pg/mL
- 回收率:91% - 107%
在售SKU:70-EK296/2-48, 70-EK296/2-96
描述
商品名 |
Mouse CXCL1/KC ELISA Kit (小鼠CXCL1/KC ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:3.9% - 5.6%;板间变异系数:4.4% - 5.9% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
20 μL |
灵敏度 |
0.47 pg/mL |
检测范围 |
31.25 pg/mL - 2000 pg/mL |
回收率 |
91% - 107% |
平均回收率 |
0.98 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠CXCL1抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的CXCL1与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中CXCL1的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
CXCL1 分子靶点信息概述
- 分子名:CXCL1, C-X-C motif chemokine ligand 1
- 基因家族:Chemokine ligands
- 别名:SCYB1; GROa; MGSA-a; NAP-3
- 曾用名:MGSA; GRO1; FSP
- 全称:melanoma growth stimulating activity, alpha; GRO1 oncogene (melanoma growth stimulating activity, alpha); fibroblast secretory protein; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
CXCL1 分子靶点综述
趋化因子(C-X-C基序)配体1(CXCL1)是一个小分子量细胞因子,属于CXC趋化因子家族,以前又被称为GRO1致癌基因、GROα、KC和中性粒细胞激活蛋白3 (NAP-3)。在人类中,该蛋白由CXCL1基因编码。CXCL1由人类黑色素瘤细胞分泌,具有促有丝分裂特性,且与黑色素瘤发病机制有关。CXCL1由巨噬细胞、中性粒细胞和上皮细胞表达,有中性粒细胞趋化活性。该趋化因子通过趋化因子受体CXCR2产生信号,进而引发它的作用效果。CXCL1通过抑制少突细胞前体的迁移,从而对脊髓发育起作用。它参与血管生长、炎症、伤口愈合和肿瘤发生过程。小鼠的初步研究表明,CXCL1可降低多发性硬化症的严重程度,可提供神经保护作用。
小鼠 Mouse Cxcl1 分子靶点信息
- 分子名:Cxcl1, chemokine (C-X-C motif) ligand 1
- 别称:
- fibroblast secretory protein
- Fsp
- Gro1
- GRO1 oncogene
- KC
- KC/GRO-alpha
- melanoma growth stimulatory activity
- Mgsa
- N51
- Scyb1
- 基因序列:NCBI_Gene: 14825
- 蛋白序列:
小鼠 Mouse Cxcl1 靶点分子功能(预测)
Predicted to enable CXCR chemokine receptor binding activity and chemokine activity. Involved in positive regulation of hematopoietic stem cell proliferation; positive regulation of neutrophil mediated killing of fungus; and positive regulation of superoxide anion generation. Located in extracellular space. Is expressed in several structures, including gonad; liver; lung; nervous system; and retina. Orthologous to human CXCL2 (C-X-C motif chemokine ligand 2).
引用文献统计
该产品被引用的文献总数为:55
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