Mouse BDNF ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:BDNF, neurotrophin
- 种属:小鼠 (Mouse)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:10 μL
- 灵敏度:1.05 pg/mL
- 检测范围:15.63 pg/mL - 1000 pg/mL
- 回收率:75% - 126%
在售SKU:70-EK2127-48, 70-EK2127-96
描述
商品名 |
Mouse BDNF ELISA Kit (小鼠脑源性神经营养因子 (BDNF) ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:2.7% - 4.4%;板间变异系数:3.9% - 4.9% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
10 μL |
灵敏度 |
1.05 pg/mL |
检测范围 |
15.63 pg/mL - 1000 pg/mL |
回收率 |
75% - 126% |
平均回收率 |
1.01 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠BDNF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的BDNF与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中BDNF的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
BDNF 分子靶点信息概述
- 分子名:BDNF, brain derived neurotrophic factor
- 基因家族:Neurotrophins
- 全称:neurotrophin
BDNF 分子靶点综述
脑源性神经营养因子(BDNF)是生长因子神经营养素家族中的一员,与神经生长因子相关。神经营养因子存在于脑及外周。BDNF在内质网中加工合成,由致密核心囊泡分泌。它在大脑中的海马、皮层和基底前脑区域活跃,同样也在视网膜、运动神经元、肾脏、唾液和前列腺中表达。BDNF作用于中枢神经系统和外周神经系统的某些神经元,帮助现存神经元的存活、促进新生神经元和突触的生长和分化。BDNF的改变可能在精神分裂症的发病中起作用,与抑郁、湿疹、癫痫、阿尔茨海默病、肥胖、衰老、先天性中枢性低通气综合征和化疗后认知功能障碍有关。
小鼠 Mouse Bdnf 分子靶点信息
- 分子名:Bdnf, brain derived neurotrophic factor
- 别称:
- 无
- 基因序列:NCBI_Gene: 12064
- 蛋白序列:
小鼠 Mouse Bdnf 靶点分子功能(预测)
Predicted to enable growth factor activity; nerve growth factor receptor binding activity; and neurotrophin TRKB receptor binding activity. Involved in negative regulation of apoptotic signaling pathway; positive regulation of neuron projection development; and retrograde trans-synaptic signaling by neuropeptide, modulating synaptic transmission. Acts upstream of or within several processes, including generation of neurons; modulation of chemical synaptic transmission; and sensory organ development. Located in several cellular components, including dendrite; perinuclear region of cytoplasm; and secretory granule. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in several diseases, including cognitive disorder (multiple); congenital central hypoventilation syndrome; neurodegenerative disease (multiple); obstructive sleep apnea; and status epilepticus. Orthologous to human BDNF (brain derived neurotrophic factor).
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 血清OR血浆,哪个是ELISA的菜 
- ELISA通关必备丨数据篇丨标准曲线不佳 
- ELISA通关必备丨操作篇丨常见问题及解决方案 
- ELISA通关必备丨操作篇丨溶解与稀释标准品 
- ELISA通关必备丨样本篇丨不常见样本 
- ELISA通关必备丨样本篇丨常见样本丨细胞 
- ELISA通关必备丨如何选择试剂盒 
- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
- ELISA常见类型一 | 双抗夹心法,你要的都在这里! 
- ELISA常见类型二 | 竞争法,五分钟搞定! 
- 叮!联科向您投递了个ELISA实验操作干货包,请查收~ 
- 【视频】ELISA实验操作步骤演示视频教程 
- ELISA 组织样本的处理—大鼠组织 
- 【视频】ELISA实验原理与常见问题分析 
- 查看更多ELISA操作相关问题
ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- Melatonin enhances autologous adipose-derived stem cells to improve mouse ovarian function in relation to the SIRT6/NF-κB pathway 
- Tenacissoside H promotes neurological recovery of cerebral ischaemia/reperfusion injury in mice by modulating inflammation and oxidative stress via TrkB pathway 
- Duloxetine-Induced Neural Cell Death and Promoted Neurite Outgrowth in N2a Cells 
- Depletion of placental brain-derived neurotrophic factor (BDNF) is attributed to premature ovarian insufficiency (POI) in mice offspring 
- BDNF promotes mouse follicular development and reverses ovarian aging by promoting cell proliferation 
- Arctiin alleviates functional constipation by enhancing intestinal motility in mice 
Melatonin enhances autologous adipose-derived stem cells to improve mouse ovarian function in relation to the SIRT6/NF-κB pathway 
Tenacissoside H promotes neurological recovery of cerebral ischaemia/reperfusion injury in mice by modulating inflammation and oxidative stress via TrkB pathway 
Duloxetine-Induced Neural Cell Death and Promoted Neurite Outgrowth in N2a Cells 
Depletion of placental brain-derived neurotrophic factor (BDNF) is attributed to premature ovarian insufficiency (POI) in mice offspring 
BDNF promotes mouse follicular development and reverses ovarian aging by promoting cell proliferation 
Arctiin alleviates functional constipation by enhancing intestinal motility in mice 
评价
目前还没有评价