Human TRANCE/TNFSF11/RANKL ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:TNFSF11, RANKL, CD254
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:50 μL
- 灵敏度:0.75 pg/mL
- 检测范围:7.81 pg/mL - 500 pg/mL
- 回收率:80% - 102%
在售SKU:70-EK1208-48, 70-EK1208-96
描述
商品名 |
Human TRANCE/TNFSF11/RANKL ELISA Kit (人 TRANCE/TNFSF11/RANKL ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:5.6% - 8.6%;板间变异系数:2.2% - 7.7% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
50 μL |
灵敏度 |
0.75 pg/mL |
检测范围 |
7.81 pg/mL - 500 pg/mL |
回收率 |
80% - 102% |
平均回收率 |
0.91 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人TRANCE抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的TRANCE与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中TRANCE的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
TNFSF11 分子靶点信息概述
- 分子名:TNFSF11, TNF superfamily member 11
- 基因家族:CD molecules; Tumor necrosis factor superfamily
- 别名:TRANCE; RANKL; OPGL; ODF; CD254
- 全称:tumor necrosis factor (ligand) superfamily, member 11
TNFSF11 分子靶点综述
肿瘤坏死因子相关激活诱导因子(TRANCE),又名肿瘤坏死因子配体超家族成员11(TNFSF11)、核因子κB受体活化因子配基(RANKL),是TNF超家族成员。成骨细胞、CD4+和CD8+T细胞、巨核细胞、支气管和肠道上皮细胞等都可表达TRANCE。TRANCE在免疫应答和骨形态发生中起核心作用。在骨形成和重塑过程中,成骨细胞表达的TRANCE与M-CSF一起诱导破骨细胞形成和活化,导致骨吸收,进而导致随后的骨沉积和重塑。TRANCE同样在病理性骨质疏松中起作用。活化的淋巴细胞和成骨细胞不恰当表达TRANCE就会导致关节和骨头的过度吸收与沉积。过量的TRANCE与许多退行性骨疾病,如类风湿性关节炎、银屑病性关节炎有关。
人 Human TNFSF11 分子靶点信息
- 分子名:TNFSF11, TNF superfamily member 11
- 别称:
- CD254
- hRANKL2
- ODF
- OPGL
- OPTB2
- osteoclast differentiation factor
- osteoprotegerin ligand
- RANKL
- receptor activator of nuclear factor kappa B ligand
- receptor activator of nuclear factor kappa-B ligand
- sOdf
- TNF-related activation-induced cytokine
- TNLG6B
- TRANCE
- tumor necrosis factor (ligand) superfamily member 11
- tumor necrosis factor (ligand) superfamily, member 11
- tumor necrosis factor ligand 6B
- tumor necrosis factor ligand superfamily member 11
- tumor necrosis factor superfamily member 11
- 基因序列:NCBI_Gene: 8600
- 蛋白序列:UniProtKB: O14788
人 Human TNFSF11靶点分子功能(预测)
Enables cytokine activity. Involved in several processes, including positive regulation of MAPK cascade; positive regulation of cell-cell adhesion; and regulation of transcription, DNA-templated. Predicted to be located in extracellular region and plasma membrane. Implicated in autosomal recessive osteopetrosis 2 and osteoporosis. Biomarker of myocardial infarction.
操作步骤
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引用文献
文章目录[隐藏]
- Biological responses of human bone mesenchymal stem cells to Ti and TiZr implant materials 
- Piperlongumine induces apoptosis and reduces bortezomib resistance by inhibiting STAT3 in multiple myeloma cells 
- Supranutritional selenium suppresses ROS-induced generation of RANKL-expressing osteoclastogenic CD4+ T cells and ameliorates rheumatoid arthritis 
- Bone remodeling serum markers in children with systemic lupus erythematosus 
- Vertebral marrow fat fraction is associated with circulating RANKL in postmenopausal females 
- The autocrine action of salidroside on osteoclast during osteoclastogenesis via hypoxia-inducible factor-1α pathway 
- Association of serum anti-carbamylated protein antibodies with disease activity and bone loss in rheumatoid arthritis 
Biological responses of human bone mesenchymal stem cells to Ti and TiZr implant materials 
Piperlongumine induces apoptosis and reduces bortezomib resistance by inhibiting STAT3 in multiple myeloma cells 
Supranutritional selenium suppresses ROS-induced generation of RANKL-expressing osteoclastogenic CD4+ T cells and ameliorates rheumatoid arthritis 
Bone remodeling serum markers in children with systemic lupus erythematosus 
Vertebral marrow fat fraction is associated with circulating RANKL in postmenopausal females 
The autocrine action of salidroside on osteoclast during osteoclastogenesis via hypoxia-inducible factor-1α pathway 
Association of serum anti-carbamylated protein antibodies with disease activity and bone loss in rheumatoid arthritis 
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