Human TRAIL/TNFSF10 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:TNFSF10, TRAIL, TL2, CD253
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:20 μL
- 灵敏度:8.19 pg/mL
- 检测范围:125 pg/mL - 8000 pg/mL
- 回收率:77% - 120%
在售SKU:70-EK1161-48, 70-EK1161-96
描述
商品名 |
Human TRAIL/TNFSF10 ELISA Kit (人肿瘤坏死因子相关的凋亡诱导配体 (TRAIL) ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:6.6% - 8.3%;板间变异系数:4.8% - 6.4% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
20 μL |
灵敏度 |
8.19 pg/mL |
检测范围 |
125 pg/mL - 8000 pg/mL |
回收率 |
77% - 120% |
平均回收率 |
0.97 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人TRAIL抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的TRAIL与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中TRAIL的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
TNFSF10 分子靶点信息概述
- 分子名:TNFSF10, TNF superfamily member 10
- 基因家族:CD molecules; Tumor necrosis factor superfamily
- 别名:TRAIL; Apo-2L; TL2; CD253
- 全称:tumor necrosis factor (ligand) superfamily, member 10
TNFSF10 分子靶点综述
肿瘤坏死因子相关的凋亡诱导配体(TRAIL)也称为CD253或TNFSF10,可由大部分正常组织细胞产生并分泌。TRAIL与死亡受体DR4(TRAIL-RI)和DR5(TRAIL-RII)结合。凋亡的过程依赖于caspase-8。Caspase-8活化下游的caspase,包括procaspase-3、6和7,使特异性的激酶活化。TRAIL也能与不含胞浆区的受体DcR1和含有截短的死亡结构域的受体DcR2结合。DcR1可作为TRAIL中和受体。DcR2的胞浆区具有功能,可活化NF-κB。因此在表达DcR2的细胞中,TRAIL的结合会活化NF-κB,使拮抗死亡信号通路和/或促进炎症的基因发生转录。
人 Human TNFSF10 分子靶点信息
- 分子名:TNFSF10, TNF superfamily member 10
- 别称:
- Apo-2 ligand
- Apo-2L
- APO2L
- CD253
- chemokine tumor necrosis factor ligand superfamily member 10
- TL2
- TNF-related apoptosis inducing ligand TRAIL
- TNF-related apoptosis-inducing ligand
- TNLG6A
- TRAIL
- tumor necrosis factor (ligand) family, member 10
- tumor necrosis factor (ligand) superfamily member 10
- tumor necrosis factor (ligand) superfamily, member 10
- tumor necrosis factor apoptosis-inducing ligand splice variant delta
- tumor necrosis factor ligand 6A
- tumor necrosis factor ligand superfamily member 10
- tumor necrosis factor superfamily member 10
- 基因序列:NCBI_Gene: 8743
- 蛋白序列:UniProtKB: P50591
人 Human TNFSF10靶点分子功能(预测)
Enables identical protein binding activity and zinc ion binding activity. Involved in positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; positive regulation of release of cytochrome c from mitochondria; and positive regulation of signal transduction. Located in extracellular exosome. Implicated in arteriosclerosis and small cell carcinoma. Biomarker of type 1 diabetes mellitus.
操作步骤
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引用文献
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