EK182

Human TNF-a ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥10,800.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：TNF, TNFA, TNFSF2
  • 种属：人 (Human)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：50 μL
  • 灵敏度：0.42 pg/mL
  • 检测范围：15.63 pg/mL - 1000 pg/mL
  • 回收率：89% - 97%

在售SKU:70-EK182-48, 70-EK182-96

描述

商品名

Human TNF-α ELISA Kit (人肿瘤坏死因子α (TNF-α) ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：3.8% - 8.9%；板间变异系数：5.0% - 8.4%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

50 μL
灵敏度

0.42 pg/mL
检测范围

15.63 pg/mL - 1000 pg/mL
回收率

89% - 97%
平均回收率

0.93
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗TNF-α单克隆抗体的96孔聚苯乙烯酶标板
  • 人TNF-α冻干标准品
  • TNF-α检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人TNF-α抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的TNF-α与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中TNF-α的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human TNF-α ELISA Kit (人肿瘤坏死因子α (TNF-α) ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 TNF, TNFA, TNFSF2 靶点信息

TNF 分子靶点信息概述

  • 分子名：TNF, tumor necrosis factor
  • 基因家族：Tumor necrosis factor superfamily
  • 别名：TNFSF2; DIF; TNF-alpha
  • 曾用名：TNFA
  • 全称：TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)

TNF 分子靶点综述

TNF-α 为单核细胞、巨噬细胞、中性粒细胞、NK 细胞和 CD4+T 细胞产生的 17 kDa 细胞因子。它在免疫应答中具有多种调节功能，还可作为潜在的热原。TNF-α对刺激物(感染因子或组织受损)产生应答后，在全身循环，激活中性粒细胞，改变血管内皮细胞的特性，调控其它组织的代谢活性，以及通过诱导局部凝血作用展现肿瘤杀伤活性。TNF-α 在关节组织和其它组织发生炎症的发病机制具有重要作用。

人 Human TNF 分子靶点信息

  • 分子名：TNF, tumor necrosis factor
  • 别称：
    • APC1 protein
    • cachectin
    • DIF
    • TNF superfamily, member 2
    • TNF-a
    • TNF-alpha
    • TNF, macrophage-derived
    • TNF, monocyte-derived
    • TNFA
    • TNFSF2
    • TNLG1F
    • tumor necrosis factor alpha
    • tumor necrosis factor ligand 1F
    • tumor necrosis factor ligand superfamily member 2
    • tumor necrosis factor-alpha
  • 基因序列：NCBI_Gene: 7124
  • 蛋白序列：UniProtKB: P01375

人 Human TNF靶点分子功能（预测）

Enables several functions, including identical protein binding activity; protease binding activity; and signaling receptor binding activity. Involved in several processes, including negative regulation of macromolecule metabolic process; positive regulation of macromolecule metabolic process; and regulation of apoptotic process. Acts upstream of or within several processes, including regulation of cellular protein metabolic process; regulation of gene expression; and regulation of signal transduction. Located in cell surface; extracellular space; and membrane raft. Is integral component of plasma membrane. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Biomarker of several diseases, including anemia (multiple); autoimmune disease (multiple); eye disease (multiple); kidney disease (multiple); and lung disease (multiple).

操作步骤


下载本产品说明书

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

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【视频】ELISA实验操作步骤演示视频教程 

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引用文献

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  4. Construction of PD1/CD28 chimeric-switch receptor enhances anti-tumor ability of c-Met CAR-T in gastric cancer 

  5. Timosaponin B-II alleviates osteoarthritis-related inflammation and extracellular matrix degradation through inhibition of mitogen-activated protein kinases and nuclear factor-κB pathways in vitro 

  6. Consumption of Saturated Fatty Acids-Rich Lard Benefits Recovery of Experimental Arthritis by Activating PPAR-γ 

  7. Overexpression of TOLLIP Protects against Acute Kidney Injury after Paraquat Intoxication through Inhibiting NLRP3 Inflammasome Activation Modulated by Toll-Like Receptor 2/4 Signaling 

  8. Down-regulation of long non-coding RNA XIST aggravates sepsis-induced lung injury by regulating miR-16-5p 

  9. Smoking increases oral mucosa susceptibility to Candida albicans infection via the Nrf2 pathway: In vitro and animal studies 

  10. Chronic exposure to microcystin-leucine-arginine induces epithelial hyperplasia and inflammation in the mouse bladder 

  11. Oral Core–Shell Nanoparticles Embedded in Hydrogel Microspheres for the Efficient Site-Specific Delivery of Magnolol and Enhanced Antiulcerative Colitis Therapy 

  12. Biomimetic Macrophage Membrane and Lipidated Peptide Hybrid Nanovesicles for Atherosclerosis Therapy 

  13. miR-146a is directly regulated by STAT3 in human hepatocellular carcinoma cells and involved in anti-tumor immune suppression 

  14. Dimethyl fumarate ameliorates lipopolysaccharide-induced acute lung injury by inhibiting NLRP3 inflammasome-mediated pyroptosis through enhancing Nrf2 signaling 

  15. Paclitaxel alleviated sepsis-induced acute lung injury by activating MUC1 and suppressing TLR-4/NF-κB pathway 

  16. Ginsenoside Rg3 suppresses the NLRP3 inflammasome activation through inhibition of its assembly 

  17. MiR-128-3p inhibits vascular smooth muscle cell proliferation and migration by repressing FOXO4/MMP9 signaling pathway 

  18. Vitexin protects against ethanol-induced liver injury through Sirt1/p53 signaling pathway 

  19. MiR-29c protects against inflammation and apoptosis in Parkinson's disease model in vivo and in vitro by targeting SP1 

  20. Downregulating lncRNA PRNCR1 ameliorates LPS-induced pulmonary vascular endothelial cell injury by modulating miR-330-5p/TLR4 axis 

  21. Mycobacterial PPE13 activates inflammasome by interacting with the NATCH and LRR domains of NLRP3 

  22. Platelet-derived growth factor B attenuates lethal sepsis through inhibition of inflammatory responses 

  23. Polysaccharides from Dendrobium officinale ameliorate colitis-induced lung injury via inhibiting inflammation and oxidative stress 

  24. Polysaccharides from Platycodonis Radix ameliorated respiratory syncytial virus-induced epithelial cell apoptosis and inflammation through activation of miR-181a-mediated Hippo and SIRT1 pathways 

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  27. Role of CD8+ T cell exhaustion in the progression and prognosis of acute respiratory distress syndrome induced by sepsis: a prospective observational study 

  28. Macrophage polarization toward M1 phenotype through NF-κB signaling in patients with Behçet’s disease 

  29. Puerarin Prevents Acute Liver Injury via Inhibiting Inflammatory Responses and ZEB2 Expression 

  30. Effects of Transcutaneous Electrical Acupoint Stimulation on Systemic Inflammatory Response Syndrome of Patients after Percutaneous Nephrolithotomy: A Randomized Controlled Trial 

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  32. Cytokine nanosponges suppressing overactive macrophages and dampening systematic cytokine storm for the treatment of hemophagocytic lymphohistiocytosis 

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  34. Ultrasound-excited temozolomide sonosensitization induces necroptosis in glioblastoma 

  35. Toxoplasma gondii SAG1 targeting host cell S100A6 for parasite invasion and host immunity 

  36. Systems Pharmacology Based Strategy for Q-Markers Discovery of HuangQin Decoction to Attenuate Intestinal Damage 

  37. Inflammatory Cytokines Changed in Patients With Depression Before and After Repetitive Transcranial Magnetic Stimulation Treatment 

  38. Phillygenin inhibits LPS-induced activation and inflammation of LX2 cells by TLR4/MyD88/NF-κB signaling pathway 

  39. Liraglutide regulates bone destruction and exhibits anti-inflammatory effects in periodontitis in vitro and in vivo 

  40. Gypenosides Protect Orbital Fibroblasts in Graves Ophthalmopathy via Anti-Inflammation and Anti-Fibrosis Effects 

  41. Effects of the Chinese herbal medicine Hong Huang decoction, on myocardial injury in breast cancer patients who underwent anthracycline-based chemotherapy 

  42. Inhibition of microRNA-92a ameliorates lipopolysaccharide-induced endothelial barrier dysfunction by targeting ITGA5 through the PI3K/Akt signaling pathway in human pulmonary microvascular endothelial cells 

  43. Promotion of angiogenesis in vitro by Astragalus polysaccharide via activation of TLR4 signaling pathway 

  44. Protective mechanisms of Zanthoxylum bungeanum essential oil on DSS-induced ulcerative colitis in mice based on a colonic mucosal transcriptomic approach 

  45. Atorvastatin inhibits renal inflammatory response induced by calcium oxalate crystals via inhibiting the activation of TLR4/NF-κB and NLRP3 inflammasome 

  46. Development, characterisation, and in vitro anti-tumor effect of self-microemulsifying drug delivery system containing polyphyllin I 

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  49. Vitamin D deficiency is associated with thyroid autoimmunity: results from an epidemiological survey in Tianjin, China 

  50. Original research: Shed antigen-induced blocking effect on CAR-T cells targeting Glypican-3 in Hepatocellular Carcinoma 

  51. NLRP3 inflammasome inhibitor INF39 attenuated NLRP3 assembly in macrophages 

  52. Baicalein inhibits inflammatory response and promotes osteogenic activity in periodontal ligament cells challenged with lipopolysaccharides 

  53. Helicobacter pylori tumor necrosis factor-α inducing protein promotes cytokine expression via nuclear factor-κB 

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  80. MicroRNA-181b stimulates inflammation via the nuclear factor-κB signaling pathway in vitro 

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