Human Thrombospondin-1/THBS-1 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:THBS1, THBS, TSP1, TSP
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:100 μL
- 灵敏度:26.39 pg/mL
- 检测范围:0.78 ng/mL - 50 ng/mL
- 回收率:85% - 110%
在售SKU:70-EK1155-48, 70-EK1155-96
描述
商品名 |
Human Thrombospondin-1/THBS-1 ELISA Kit (人血小板反应素-1 (THBS-1) ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:2.3% - 2.5%;板间变异系数:3.5% - 5.6% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
100 μL |
灵敏度 |
26.39 pg/mL |
检测范围 |
0.78 ng/mL - 50 ng/mL |
回收率 |
85% - 110% |
平均回收率 |
0.99 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人THBS-1抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的THBS-1与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中THBS-1的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
THBS1 分子靶点信息概述
- 分子名:THBS1, thrombospondin 1
- 别名:TSP1; THBS; TSP; THBS-1; TSP-1
- 全称:thrombospondin-1p180
THBS1 分子靶点综述
血小板反应素-1(THBS-1)是血小板反应素家族成员,介导细胞-细胞和细胞-基质相互作用。该蛋白可与纤维蛋白原、纤维连接蛋白、层粘连蛋白、V型胶原和整合素α-V/β-1结合。它对血小板聚集、血管生成和肿瘤发生发挥作用。在正常组织中,它是新血管形成和肿瘤发生的天然抑制剂。THBS-1还可正向和负向调控内皮细胞黏附、运动和生长。通过阻断THBS-1与细胞表面受体(CD47)结合,正常组织几乎对肿瘤辐射治疗产生免疫,并有助肿瘤死亡。
人 Human THBS1 分子靶点信息
- 分子名:THBS1, thrombospondin 1
- 别称:
- glycoprotein G
- THBS
- THBS-1
- thrombospondin-1
- thrombospondin-1p180
- thrombospondin-p50
- TSP
- TSP-1
- TSP1
- 基因序列:NCBI_Gene: 7057
- 蛋白序列:UniProtKB: P07996
人 Human THBS1靶点分子功能(预测)
Enables several functions, including collagen V binding activity; fibrinogen binding activity; and fibroblast growth factor binding activity. Involved in several processes, including regulation of cell migration; regulation of gene expression; and regulation of signal transduction. Acts upstream of or within several processes, including negative regulation of endothelial cell chemotaxis; negative regulation of endothelial cell proliferation; and negative regulation of nitric oxide mediated signal transduction. Located in several cellular components, including external side of plasma membrane; extracellular space; and platelet alpha granule. Part of fibrinogen complex. Colocalizes with collagen-containing extracellular matrix. Implicated in cholangiocarcinoma; pancreatic cancer; and pancreatic ductal carcinoma. Biomarker of COVID-19; cholangiocarcinoma; obesity; pancreatic cancer (multiple); and urinary bladder cancer.
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 血清OR血浆,哪个是ELISA的菜 
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- 真?假?ELISA试剂盒选择要小心 
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引用文献
文章目录[隐藏]
- Decreased serum thrombospondin-1 and elevation of its autoantibody are associated with multiple exacerbated clinical manifestations in systemic lupus erythematosus 
- Elevated cerebrospinal fluid levels of thrombospondin-1 correlate with adverse clinical outcome in patients with aneurysmal subarachnoid hemorrhage 
- Study on diagnostic-sensitive markers of primary immune thrombocytopaenia in children based on plasma proteomics 
Decreased serum thrombospondin-1 and elevation of its autoantibody are associated with multiple exacerbated clinical manifestations in systemic lupus erythematosus 
Elevated cerebrospinal fluid levels of thrombospondin-1 correlate with adverse clinical outcome in patients with aneurysmal subarachnoid hemorrhage 
Study on diagnostic-sensitive markers of primary immune thrombocytopaenia in children based on plasma proteomics 
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