EK1155

Human Thrombospondin-1/THBS-1 ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:THBS1, THBS, TSP1, TSP
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:100 μL
  • 灵敏度:26.39 pg/mL
  • 检测范围:0.78 ng/mL - 50 ng/mL
  • 回收率:85% - 110%

在售SKU:70-EK1155-48, 70-EK1155-96


描述

商品名

Human Thrombospondin-1/THBS-1 ELISA Kit (人血小板反应素-1 (THBS-1) ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.3% - 2.5%;板间变异系数:3.5% - 5.6%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

100 μL

灵敏度

26.39 pg/mL

检测范围

0.78 ng/mL - 50 ng/mL

回收率

85% - 110%

平均回收率

0.99

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗Thrombospondin-1/THBS-1单克隆抗体的96孔聚苯乙烯酶标板
  • 人Thrombospondin-1/THBS-1冻干标准品
  • Thrombospondin-1/THBS-1检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人THBS-1抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的THBS-1与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中THBS-1的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human Thrombospondin-1/THBS-1 ELISA Kit (人血小板反应素-1 (THBS-1) ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 THBS1, THBS, TSP1, TSP 靶点信息

THBS1 分子靶点信息概述

  • 分子名:THBS1, thrombospondin 1
  • 别名:TSP1; THBS; TSP; THBS-1; TSP-1
  • 全称:thrombospondin-1p180

THBS1 分子靶点综述

血小板反应素-1(THBS-1)是血小板反应素家族成员,介导细胞-细胞和细胞-基质相互作用。该蛋白可与纤维蛋白原、纤维连接蛋白、层粘连蛋白、V型胶原和整合素α-V/β-1结合。它对血小板聚集、血管生成和肿瘤发生发挥作用。在正常组织中,它是新血管形成和肿瘤发生的天然抑制剂。THBS-1还可正向和负向调控内皮细胞黏附、运动和生长。通过阻断THBS-1与细胞表面受体(CD47)结合,正常组织几乎对肿瘤辐射治疗产生免疫,并有助肿瘤死亡。

人 Human THBS1 分子靶点信息

  • 分子名:THBS1, thrombospondin 1
  • 别称:
    • glycoprotein G
    • THBS
    • THBS-1
    • thrombospondin-1
    • thrombospondin-1p180
    • thrombospondin-p50
    • TSP
    • TSP-1
    • TSP1
  • 基因序列:NCBI_Gene: 7057
  • 蛋白序列:UniProtKB: P07996

人 Human THBS1靶点分子功能(预测)

Enables several functions, including collagen V binding activity; fibrinogen binding activity; and fibroblast growth factor binding activity. Involved in several processes, including regulation of cell migration; regulation of gene expression; and regulation of signal transduction. Acts upstream of or within several processes, including negative regulation of endothelial cell chemotaxis; negative regulation of endothelial cell proliferation; and negative regulation of nitric oxide mediated signal transduction. Located in several cellular components, including external side of plasma membrane; extracellular space; and platelet alpha granule. Part of fibrinogen complex. Colocalizes with collagen-containing extracellular matrix. Implicated in cholangiocarcinoma; pancreatic cancer; and pancreatic ductal carcinoma. Biomarker of COVID-19; cholangiocarcinoma; obesity; pancreatic cancer (multiple); and urinary bladder cancer.

操作步骤

ELISA操作常见问题

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引用文献


  1. Decreased serum thrombospondin-1 and elevation of its autoantibody are associated with multiple exacerbated clinical manifestations in systemic lupus erythematosus 

  2. Elevated cerebrospinal fluid levels of thrombospondin-1 correlate with adverse clinical outcome in patients with aneurysmal subarachnoid hemorrhage 

  3. Study on diagnostic-sensitive markers of primary immune thrombocytopaenia in children based on plasma proteomics 

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