EK186S

Human sP-Selectin Standard (人可溶性P选择素 标准品)

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¥280.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:SELP, CD62P, GRMP
  • 种属:人 (Human)
  • 试剂盒:EK186
  • 保存:短期4℃保存,长期-20℃保存
  • 运输条件:4℃蓝冰运输

在售SKU:70-EK186S


描述

本产品只包含标准品试剂,如需购买试剂盒请点击下图

商品名

Human sP-Selectin Standard (人可溶性P选择素 标准品)

组分

人可溶性P选择素标准品

检测方法

双抗夹心法

样本类型

血清,血浆,细胞培养上清及其他生物学样本

板式

保存

短期4℃,长期-20℃保存

运输条件

4℃蓝冰运输

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人sP-Selectin抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的sP-Selectin与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中sP-Selectin的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human sP-Selectin ELISA Kit (人可溶性P选择素 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 SELP, CD62P, GRMP 靶点信息

SELP 分子靶点信息概述

  • 分子名:SELP, selectin P
  • 基因家族:CD molecules; Selectins; Sushi domain containing; C-type lectin domain containing
  • 别名:CD62; PSEL; PADGEM; GMP140; CD62P
  • 曾用名:GRMP
  • 全称:antigen CD62; granule membrane protein 140kDa; selectin P (granule membrane protein 140kD, antigen CD62); selectin P (granule membrane protein 140kDa, antigen CD62)

SELP 分子靶点综述

CD62P 是一种 140kDa 跨膜糖蛋白,也称为 P-Selectin。P-Selectin被储存在血小板的 α 颗粒和内皮细胞的 Weibel-Palade 小体中,并在激活后迅速转运到质膜。在炎症反应中,P-Selectin 被认为介导中性粒细胞和单核细胞与内皮的初始粘附相互作用;在止血过程中,活化的血小板与中性粒细胞和单核细胞相互作用。

人 Human SELP 分子靶点信息

  • 分子名:SELP, selectin P
  • 别称:
    • CD62
    • CD62 antigen-like family member P
    • CD62P
    • FLJ45155
    • GMP-140
    • GMP140
    • granule membrane protein 140
    • granule membrane protein 140kDa
    • granulocyte membrane protein
    • GRMP
    • LECAM3
    • leukocyte-endothelial cell adhesion molecule 3
    • P-selectin
    • PADGEM
    • platelet activation dependent granule-external membrane protein
    • platelet alpha-granule membrane protein
    • PSEL
    • selectin P (granule membrane protein 140kDa, antigen CD62)
  • 基因序列:NCBI_Gene: 6403
  • 蛋白序列:UniProtKB: P16109

人 Human SELP靶点分子功能(预测)

Enables several functions, including fucose binding activity; heparin binding activity; and lipopolysaccharide binding activity. Involved in several processes, including calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules; calcium-mediated signaling using intracellular calcium source; and leukocyte tethering or rolling. Located in external side of plasma membrane; extracellular space; and platelet alpha granule membrane. Is integral component of plasma membrane. Implicated in atopic dermatitis and myocardial infarction. Biomarker of several diseases, including Kawasaki disease; autoimmune disease (multiple); coronary artery disease (multiple); obesity; and type 2 diabetes mellitus.

操作步骤


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引用文献


  1. miR-374b-5p is increased in deep vein thrombosis and negatively targets IL-10 

  2. Thymic stromal lymphopoietin induces platelet mitophagy and promotes thrombosis in Kawasaki disease 

  3. Salvianolic acid B inhibits platelets-mediated inflammatory response in vascular endothelial cells 

  4. A metal-organic-framework incorporated vascular graft for sustained nitric oxide generation and long-term vascular patency 

  5. Direct thrombin inhibitor-bivalirudin improved the hemocompatibility of electrospun polycaprolactone vascular grafts 

  6. LncRNA MALAT1 suppresses monocyte-endothelial cell interactions by targeting miR-30b-5p and enhancing ATG5-mediated autophagy 

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