Human Serpin E1/PAI-1 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:SERPINE1, PAI1
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞上清
- 检测样本体积:100 μL
- 灵敏度:1.53 pg/mL
- 检测范围:0.31 ng/mL - 20 ng/mL
- 回收率:80% - 118%
在售SKU:70-EK1136-48, 70-EK1136-96
描述
商品名 |
Human Serpin E1/PAI-1 ELISA Kit (人Serpin E1/纤溶酶原激活物抑制因子-1 (PAI-1) ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:2.3% - 4.8%;板间变异系数:2.3% - 2.6% |
样本类型 |
血清,血浆,细胞上清 |
检测样本体积 |
100 μL |
灵敏度 |
1.53 pg/mL |
检测范围 |
0.31 ng/mL - 20 ng/mL |
回收率 |
80% - 118% |
平均回收率 |
0.9 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人Serpin E1抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的Serpin E1与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中Serpin E1的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
SERPINE1 分子靶点信息概述
- 分子名:SERPINE1, serpin family E member 1
- 基因家族:Serpin peptidase inhibitors
- 别名:PAI
- 曾用名:PLANH1; PAI1
- 全称:plasminogen activator inhibitor, type I; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
SERPINE1 分子靶点综述
Serpin E1,又名纤溶酶原激活物抑制因子-1 (PAI-1),是丝氨酸蛋白酶抑制剂超家族中的一员,主要由内皮(血管内皮衬里细胞)产生,但也由其他类型的组织如脂肪组织分泌产生。Serpin E1是尿激酶和组织型纤溶酶原激活剂(uPA和tPA)的主要抑制剂。PA-血纤维蛋白溶酶系统参与多种生理和病理过程如纤维蛋白溶解、纤维化、血管生成、伤口愈合、肿瘤细胞的侵袭和转移。例如,高水平的uPA和Serpin E1与乳腺癌患者的预后不良相关,研究证实uPA和Serpin E1的临床价值处于1级水平,是最可靠的生物标志物。除了乳腺癌,Serpin E1也参与许多其它疾病如代谢综合征、哮喘、重度抑郁症(MDD)以及衰老相关疾病。血浆中Serpin E1的缺失将导致异常出血,高水平的Serpin E1与遗传性易栓症相关。
人 Human SERPINE1 分子靶点信息
- 分子名:SERPINE1, serpin family E member 1
- 别称:
- endothelial plasminogen activator inhibitor
- PAI
- PAI-1
- PAI1
- PLANH1
- plasminogen activator inhibitor 1
- serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
- serpin E1
- serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
- 基因序列:NCBI_Gene: 5054
- 蛋白序列:UniProtKB: P05121
人 Human SERPINE1靶点分子功能(预测)
Enables protease binding activity; serine-type endopeptidase inhibitor activity; and signaling receptor binding activity. Involved in several processes, including negative regulation of proteolysis; regulation of angiogenesis; and response to bacterium. Located in collagen-containing extracellular matrix and extracellular exosome. Implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); diabetic retinopathy (multiple); liver disease (multiple); and lung disease (multiple). Biomarker of several diseases, including artery disease (multiple); autoimmune disease (multiple); diabetic retinopathy (multiple); lung disease (multiple); and sleep apnea (multiple).
操作步骤
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引用文献
文章目录[隐藏]
- Knockdown of lncRNA NORAD inhibits the proliferation, inflammation and fibrosis of human mesangial cells under high‑glucose conditions by regulating the miR‑485/NRF1 axis 
- Liraglutide suppresses non-esterified free fatty acids and soluble vascular cell adhesion molecule-1 compared with metformin in patients with recent-onset type 2 diabetes 
- Helicobacter pylori-induced fibroblast-derived Serpin E1 promotes gastric cancer growth and peritoneal dissemination through p38 MAPK/VEGFA-mediated angiogenesis 
Knockdown of lncRNA NORAD inhibits the proliferation, inflammation and fibrosis of human mesangial cells under high‑glucose conditions by regulating the miR‑485/NRF1 axis 
Liraglutide suppresses non-esterified free fatty acids and soluble vascular cell adhesion molecule-1 compared with metformin in patients with recent-onset type 2 diabetes 
Helicobacter pylori-induced fibroblast-derived Serpin E1 promotes gastric cancer growth and peritoneal dissemination through p38 MAPK/VEGFA-mediated angiogenesis 
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