EK1101

Human sCD14 ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:CD14
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:100 μL
  • 灵敏度:2.58 pg/mL
  • 检测范围:125 pg/mL - 8000 pg/mL
  • 回收率:96% - 110%

在售SKU:70-EK1101-48, 70-EK1101-96, 人, 可溶性, CD14


描述

商品名

Human sCD14 ELISA Kit (人可溶性CD14 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:3.2% - 4.8%;板间变异系数:2.7% - 4.6%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

100 μL

灵敏度

2.58 pg/mL

检测范围

125 pg/mL - 8000 pg/mL

回收率

96% - 110%

平均回收率

1.03

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗sCD14单克隆抗体的96孔聚苯乙烯酶标板
  • 人sCD14冻干标准品
  • sCD14检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人sCD14抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的sCD14与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中sCD14的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human sCD14 ELISA Kit (人可溶性CD14 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 CD14 靶点信息

CD14 分子靶点信息概述

  • 分子名:CD14, CD14 molecule
  • 基因家族:CD molecules; Scavenger receptors
  • 全称:CD14 antigen

CD14 分子靶点综述

CD14是表达于单核细胞、滤泡间巨噬细胞和树突细胞亚群表面的糖蛋白。CD14与MD-2(LY)和TLR4形成受体复合物,可特异性地对LPS产生应答、传导信号。CD14/MD-2/TLR4受体复合物通过MyD88、TIRAP和TRAF6传导信号,最后激活NF-κB。

人 Human CD14 分子靶点信息

  • 分子名:CD14, CD14 molecule
  • 别称:
    • cd14 antigen
    • monocyte differentiation antigen CD14
    • myeloid cell-specific leucine-rich glycoprotein
  • 基因序列:NCBI_Gene: 929
  • 蛋白序列:UniProtKB: P08571

人 Human CD14靶点分子功能(预测)

Enables lipopeptide binding activity; lipopolysaccharide binding activity; and lipoteichoic acid binding activity. Involved in cellular response to bacterial lipopeptide; positive regulation of NIK/NF-kappaB signaling; and positive regulation of interleukin-8 production. Acts upstream of or within cellular response to lipopolysaccharide; cellular response to lipoteichoic acid; and positive regulation of tumor necrosis factor production. Located in Golgi apparatus and membrane raft. Is anchored component of external side of plasma membrane. Part of lipopolysaccharide receptor complex. Implicated in several diseases, including IgA glomerulonephritis; artery disease (multiple); celiac disease; cystic fibrosis; and pulmonary tuberculosis. Biomarker of several diseases, including biliary atresia; cystic fibrosis; lung disease (multiple); perinatal necrotizing enterocolitis; and polycystic kidney disease (multiple).

操作步骤


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引用文献


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  2. Downregulation of CD151 restricts VCAM-1 mediated leukocyte infiltration to reduce neurobiological injuries after experimental stroke 

  3. Oral Microbiota Is Associated With Immune Recovery in Human Immunodeficiency Virus-Infected Individuals 

  4. Altered gut microbiota correlate with different immune responses to HAART in HIV-infected individuals 

  5. The novel biomarkers for assessing clinical benefits of continuous renal replacement therapy in pediatric sepsis: a pilot study 

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