Human Pentraxin 3/TSG-14 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:PTX3, TSG-14, TNFAIP5
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:10 μL
- 灵敏度:0.56 pg/mL
- 检测范围:31.25 pg/mL - 2000 pg/mL
- 回收率:75% - 117%
在售SKU:70-EK1157-48, 70-EK1157-96
描述
商品名 |
Human Pentraxin 3/TSG-14 ELISA Kit (人穿透素3/肿瘤坏死因子诱导基因14 ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:2.9% - 6.3%;板间变异系数:2.9% - 3.8% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
10 μL |
灵敏度 |
0.56 pg/mL |
检测范围 |
31.25 pg/mL - 2000 pg/mL |
回收率 |
75% - 117% |
平均回收率 |
0.93 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人Pentraxin 3抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的Pentraxin 3与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中Pentraxin 3的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
PTX3 分子靶点信息概述
- 分子名:PTX3, pentraxin 3
- 基因家族:Long pentraxins
- 别名:TSG-14
- 曾用名:TNFAIP5
- 全称:long pentraxin 3; pentaxin-related gene, rapidly induced by IL-1 beta; tumor necrosis factor, alpha-induced protein 5; pentraxin-related gene, rapidly induced by IL-1 beta; pentraxin 3, long
PTX3 分子靶点综述
穿透素3(Pentraxin3)也称为肿瘤坏死因子诱导基因14(TSG-14),是穿透素超家族成员,具有环状多聚体结构。多种类型的细胞,尤其是单核吞噬细胞、树突细胞、成纤维细胞和内皮细胞,对初次炎症信号(如toll样受体、TNF-α、IL-1β)产生应答后,可快速产生并释放Pentraxin3。Pentraxin3是急性期应答蛋白,其在正常情况下含量较低(在小鼠中约25ng/ml,在人类中小于2ng/ml),而在内毒素性休克、败血症和其它炎症和感染条件下,急剧升高(诱导后6-8小时达到峰值),含量可达200-800ng/ml,与疾病的严重程度相关。在这些情况下,Pentraxin3是先天免疫和炎症反应局部活化的快速判断标志。研究显示Pentraxin3具有双重作用:保护机体抵抗病原体和调控自身免疫。
人 Human PTX3 分子靶点信息
- 分子名:PTX3, pentraxin 3
- 别称:
- long pentraxin 3
- pentaxin-related gene, rapidly induced by il-1 beta
- pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor, alpha-induced protein 5
- pentaxin-related protein PTX3
- pentraxin 3, long
- pentraxin-3
- pentraxin-related gene, rapidly induced by IL-1 beta
- pentraxin-related protein PTX3
- TNF alpha-induced protein 5
- TNFAIP5
- TSG-14
- tumor necrosis factor alpha-induced protein 5
- tumor necrosis factor-inducible gene 14 protein
- tumor necrosis factor-inducible protein TSG-14
- tumor necrosis factor, alpha-induced protein 5
- 基因序列:NCBI_Gene: 5806
- 蛋白序列:UniProtKB: P26022
人 Human PTX3靶点分子功能(预测)
Enables complement component C1q complex binding activity; identical protein binding activity; and virion binding activity. Involved in innate immune response; modulation by host of viral process; and negative regulation of viral entry into host cell. Located in extracellular space. Implicated in cystic fibrosis and invasive aspergillosis. Biomarker of hepatitis C; invasive aspergillosis; pelvic inflammatory disease; pulmonary aspergilloma; and toxic shock syndrome.
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 血清OR血浆,哪个是ELISA的菜 
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- ELISA通关必备丨如何选择试剂盒 
- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
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引用文献
文章目录[隐藏]
- Simvastatin reduces pentraxin 3 expression and secretion in human endothelial cells 
- Tanshinone IIA attenuates TNF-α induced PTX3 expression and monocyte adhesion to endothelial cells through the p38/NF-κB pathway 
- Enhanced T cell immune activity mediated by Drp1 promotes the efficacy of PD-1 inhibitors in treating lung cancer 
Simvastatin reduces pentraxin 3 expression and secretion in human endothelial cells 
Tanshinone IIA attenuates TNF-α induced PTX3 expression and monocyte adhesion to endothelial cells through the p38/NF-κB pathway 
Enhanced T cell immune activity mediated by Drp1 promotes the efficacy of PD-1 inhibitors in treating lung cancer 
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