Human Osteopontin/OPN Standard (人骨桥蛋白 标准品)
¥180.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
描述
文章目录[隐藏]
本产品只包含标准品试剂,如需购买试剂盒请点击下图
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- EK1135
- ELISA试剂盒
Human osteopontin/OPN ELISA Kit检测试剂盒(酶联免疫吸附法)
- ¥1,600.00 – ¥2,650.00
商品名 |
Human Osteopontin/OPN Standard (人骨桥蛋白 标准品) |
---|---|
组分 |
人Osteopontin/OPN 标准品 |
检测方法 |
双抗夹心法 |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
板式 |
管 |
保存 |
短期4℃,长期-20℃保存 |
运输条件 |
4℃蓝冰运输 |
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人OPN抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的OPN与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中OPN的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
SPP1 分子靶点信息概述
- 分子名:SPP1, secreted phosphoprotein 1
- 基因家族:Receptor ligands; SIBLING family
- 别名:BSPI; ETA-1
- 曾用名:BNSP; OPN
- 全称:early T-lymphocyte activation 1; osteopontin; bone sialoprotein I
SPP1 分子靶点综述
骨桥蛋白(OPN),又名BSP-1、ETA-1、SPP-1或Ric,在人类中由SPP1基因编码,主要在骨骼中表达。OPN基因的调节机制还未完全阐明,不同细胞类型的调节机理是不同的。OPN在生物矿化、骨重塑、免疫功能、趋化、细胞活化和凋亡上有作用,同样与心肌细胞凋亡和心肌功能紊乱的增加有关。OPN与许多生理和病理过程包括伤口愈合、骨转换、肿瘤发生、炎症、缺血和免疫应答有关。调控血浆(或局部)的OPN水平对治疗自身免疫性疾病、癌症转移、骨和牙齿的矿化、骨质疏松症和一些应激形式有帮助。
人 Human SPP1 分子靶点信息
- 分子名:SPP1, secreted phosphoprotein 1
- 别称:
- BNSP
- bone sialoprotein 1
- bone sialoprotein I
- BSPI
- early T-lymphocyte activation 1
- ETA-1
- MGC110940
- nephropontin
- OPN
- osteopontin
- osteopontin-C
- osteopontin-D
- osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein
- secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
- secreted phosphoprotein 1 variant 6
- secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
- SPP-1
- SPP1/CALPHA1 fusion
- urinary stone protein
- uropontin
- 基因序列:NCBI_Gene: 6696
- 蛋白序列:UniProtKB: P10451
人 Human SPP1靶点分子功能(预测)
Enables integrin binding activity. Involved in several processes, including androgen catabolic process; cellular response to testosterone stimulus; and positive regulation of estradiol secretion. Located in Golgi apparatus and extracellular space. Implicated in several diseases, including autoimmune disease (multiple); biliary atresia; coronary artery disease (multiple); disease of cellular proliferation (multiple); and hepatitis. Biomarker of calcinosis; coronary artery disease; kidney failure; progressive osseous heteroplasia; and urinary bladder cancer.
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 血清OR血浆,哪个是ELISA的菜 
- ELISA通关必备丨数据篇丨标准曲线不佳 
- ELISA通关必备丨操作篇丨常见问题及解决方案 
- ELISA通关必备丨操作篇丨溶解与稀释标准品 
- ELISA通关必备丨样本篇丨不常见样本 
- ELISA通关必备丨样本篇丨常见样本丨细胞 
- ELISA通关必备丨如何选择试剂盒 
- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
- ELISA常见类型一 | 双抗夹心法,你要的都在这里! 
- ELISA常见类型二 | 竞争法,五分钟搞定! 
- 叮!联科向您投递了个ELISA实验操作干货包,请查收~ 
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- 【视频】ELISA实验原理与常见问题分析 
- 查看更多ELISA操作相关问题
ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- Osteopontin aggravates acute lung injury in influenza virus infection by promoting macrophages necroptosis 
- Secreted phosphoprotein 1 as a potential prognostic and immunotherapy biomarker in multiple human cancers 
- Circulating osteopontin per tumor volume as a prognostic biomarker for resectable intrahepatic cholangiocarcinoma 
- Abnormally activated OPN/integrin αVβ3/FAK signalling is responsible for EGFR-TKI resistance in EGFR mutant non-small-cell lung cancer 
- Assessment of osteopontin as an early nephrotoxicity indicator in human renal proximal tubule cells and its application in evaluating lanthanum-induced nephrotoxicity 
- MIF is a critical regulator of mononuclear phagocytic infiltration in hepatocellular carcinoma 
- Osteopontin aggravates acute lung injury in influenza virus infection by promoting macrophages necroptosis 
Osteopontin aggravates acute lung injury in influenza virus infection by promoting macrophages necroptosis 
Secreted phosphoprotein 1 as a potential prognostic and immunotherapy biomarker in multiple human cancers 
Circulating osteopontin per tumor volume as a prognostic biomarker for resectable intrahepatic cholangiocarcinoma 
Abnormally activated OPN/integrin αVβ3/FAK signalling is responsible for EGFR-TKI resistance in EGFR mutant non-small-cell lung cancer 
Assessment of osteopontin as an early nephrotoxicity indicator in human renal proximal tubule cells and its application in evaluating lanthanum-induced nephrotoxicity 
MIF is a critical regulator of mononuclear phagocytic infiltration in hepatocellular carcinoma 
- 支气管肺泡灌洗液 BALF 细胞培养上清
Osteopontin aggravates acute lung injury in influenza virus infection by promoting macrophages necroptosis 
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