Human MMP-2 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:MMP2, MMP-2, TBE-1
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:10 μL
- 灵敏度:69.14 pg/mL
- 检测范围:2 ng/mL - 128 ng/mL
- 回收率:86% - 118%
在售SKU:70-EK1M02-48, 70-EK1M02-96
描述
商品名 |
Human MMP-2 ELISA Kit (人基质金属蛋白酶2 ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:2.1% - 7.8%;板间变异系数:2.4% - 3.8% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
10 μL |
灵敏度 |
69.14 pg/mL |
检测范围 |
2 ng/mL - 128 ng/mL |
回收率 |
86% - 118% |
平均回收率 |
1.08 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人MMP-2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的MMP-2与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中MMP-2的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
MMP2 分子靶点信息概述
- 分子名:MMP2, matrix metallopeptidase 2
- 基因家族:M10 matrix metallopeptidases
- 别名:TBE-1
- 曾用名:CLG4; CLG4A
- 全称:matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
MMP2 分子靶点综述
基质金属蛋白酶2(MMP-2),又名72kDa的IV型胶原酶和明胶酶A,在人类中由MMP2基因编码。激活MMP-2需要蛋白水解加工。MMP-2在子宫内膜破裂、调节血管形成和炎症应答中起作用。MMP-2参与许多事件,如癌症进程、癌细胞浸润、细胞信号转导、新血管形成和淋巴管生成。MMP2基因突变与Torg-Winchester综合征、多发性骨溶解、关节炎综合征有关,可能与瘢痕疙瘩有关。非囊性纤维化支气管扩张中,相比于呼吸道感染铜绿假单胞菌的患者,感染流感嗜血杆菌患者的MMP-2浓度是升高的。
人 Human MMP2 分子靶点信息
- 分子名:MMP2, matrix metallopeptidase 2
- 别称:
- 72 kDa gelatinase
- 72 kDa type IV collagenase
- CLG4
- CLG4A
- collagenase type IV-A
- gelatinase A
- matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
- matrix metalloproteinase 2 (gelatinase a, 72kda gelatinase, 72kda type iv collagenase)
- matrix metalloproteinase-2
- matrix metalloproteinase-II
- MMP-2
- MMP-II
- MONA
- neutrophil gelatinase
- TBE-1
- 基因序列:NCBI_Gene: 4313
- 蛋白序列:UniProtKB: P08253
人 Human MMP2靶点分子功能(预测)
Enables metallopeptidase activity and serine-type endopeptidase activity. Involved in several processes, including cellular response to UV-A; endodermal cell differentiation; and positive regulation of vascular associated smooth muscle cell proliferation. Located in extracellular space. Colocalizes with collagen-containing extracellular matrix. Implicated in artery disease (multiple); eye disease (multiple); pseudoxanthoma elasticum; and temporal arteritis. Biomarker of several diseases, including artery disease (multiple); carcinoma (multiple); cardiomyopathy (multiple); cholesteatoma (multiple); and neurodegenerative disease (multiple).
操作步骤
文章目录[隐藏]
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- ELISA通关必备丨样本篇丨不常见样本 
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- ELISA通关必备丨如何选择试剂盒 
- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
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引用文献
文章目录[隐藏]
- Curcumin suppresses the proliferation and tumorigenicity of Cal27 by modulating cancer-associated fibroblasts of TSCC 
- MicroRNA‑675‑3p promotes esophageal squamous cell cancer cell migration and invasion 
- Odd-skipped related 1 plays a tumor suppressor role in ovarian cancer via promoting follistatin-like protein 1 transcription 
- Rab11a promotes the malignant progression of ovarian cancer by inducing autophagy 
- Rhizoma Paridis saponins suppresses vasculogenic mimicry formation and metastasis in osteosarcoma through regulating miR-520d-3p/MIG-7 axis 
- Expression and significance of autophagy genes LC3, Beclin1 and MMP-2 in endometriosis 
- Integration of Dual Targeting and Dual Therapeutic Modules Endows Self-Assembled Nanoparticles with Anti-Tumor Growth and Metastasis Functions 
- Baicalein inhibits inflammatory response and promotes osteogenic activity in periodontal ligament cells challenged with lipopolysaccharides 
- Effects of β2/aβ2 on oxLDL-induced CD36 activation in THP-1 macrophages 
- Lin28/microRNA-let-7a promotes metastasis under circumstances of hyperactive Wnt signaling in esophageal squamous cell carcinoma 
- Effect of Orthodontic Combined with Implant Repair on Aesthetic Effect and Gingival Crevicular Fluid Factor in Patients with Dentition Defect and Periodontitis 
- N-acetylgalactosaminyltransferase GALNT6 is a potential therapeutic target of clear cell renal cell carcinoma progression 
- Correlation between matrix metalloproteinase-2, matrix metalloproteinase-9, and tissue inhibitor of metalloproteinases-1 and white matter hyperintensities in patients with cerebral small vessel disease based on cranial magnetic resonance 3D imaging 
- NR2F1 overexpression alleviates trophoblast cell dysfunction by inhibiting GDF15/MAPK axis in preeclampsia 
- New link between RNH1 and E2F1: regulates the development of lung adenocarcinoma 
- PELI1 overexpression contributes to pancreatic cancer progression through upregulating ubiquitination-mediated INPP5J degradation 
- Knockdown of Galectin-9 alleviates rheumatoid arthritis through suppressing TNF-α-induced activation of fibroblast-like synoviocytes 
- Kallikrein-Related Peptidase 4 Promotes Proliferation, Migration, Invasion, and Pro-Angiogenesis of Endometrial Stromal Cells via Regulation of Brain-Derived Neurotrophic Factor Production in Endometriosis 
- Inhibition of LAMP3 mediates the protective effect of vitamin D against hypoxia/reoxygenation in trophoblast cells 
- Oncogenic Role of the NFATC2/NEDD4/FBP1 Axis in Cholangiocarcinoma 
- Expression and Significance of Autophagy Genes Lc3, Beclin1 and Mmp-2 in Endometriosis 
- Microrna?675?3p Promotes Esophageal Squamous Cell Cancer Cell Migration and Invasion 
- Correlations of Serum Vegf and Mmp-2 Levels with Clm in Crc Patients and Effects of Tace on Their Expressions 
Curcumin suppresses the proliferation and tumorigenicity of Cal27 by modulating cancer-associated fibroblasts of TSCC 
MicroRNA‑675‑3p promotes esophageal squamous cell cancer cell migration and invasion 
Odd-skipped related 1 plays a tumor suppressor role in ovarian cancer via promoting follistatin-like protein 1 transcription 
Rab11a promotes the malignant progression of ovarian cancer by inducing autophagy 
Rhizoma Paridis saponins suppresses vasculogenic mimicry formation and metastasis in osteosarcoma through regulating miR-520d-3p/MIG-7 axis 
Expression and significance of autophagy genes LC3, Beclin1 and MMP-2 in endometriosis 
Integration of Dual Targeting and Dual Therapeutic Modules Endows Self-Assembled Nanoparticles with Anti-Tumor Growth and Metastasis Functions 
Baicalein inhibits inflammatory response and promotes osteogenic activity in periodontal ligament cells challenged with lipopolysaccharides 
Effects of β2/aβ2 on oxLDL-induced CD36 activation in THP-1 macrophages 
Lin28/microRNA-let-7a promotes metastasis under circumstances of hyperactive Wnt signaling in esophageal squamous cell carcinoma 
Effect of Orthodontic Combined with Implant Repair on Aesthetic Effect and Gingival Crevicular Fluid Factor in Patients with Dentition Defect and Periodontitis 
N-acetylgalactosaminyltransferase GALNT6 is a potential therapeutic target of clear cell renal cell carcinoma progression 
Correlation between matrix metalloproteinase-2, matrix metalloproteinase-9, and tissue inhibitor of metalloproteinases-1 and white matter hyperintensities in patients with cerebral small vessel disease based on cranial magnetic resonance 3D imaging 
NR2F1 overexpression alleviates trophoblast cell dysfunction by inhibiting GDF15/MAPK axis in preeclampsia 
New link between RNH1 and E2F1: regulates the development of lung adenocarcinoma 
PELI1 overexpression contributes to pancreatic cancer progression through upregulating ubiquitination-mediated INPP5J degradation 
Knockdown of Galectin-9 alleviates rheumatoid arthritis through suppressing TNF-α-induced activation of fibroblast-like synoviocytes 
Kallikrein-Related Peptidase 4 Promotes Proliferation, Migration, Invasion, and Pro-Angiogenesis of Endometrial Stromal Cells via Regulation of Brain-Derived Neurotrophic Factor Production in Endometriosis 
Inhibition of LAMP3 mediates the protective effect of vitamin D against hypoxia/reoxygenation in trophoblast cells 
Oncogenic Role of the NFATC2/NEDD4/FBP1 Axis in Cholangiocarcinoma 
Expression and Significance of Autophagy Genes Lc3, Beclin1 and Mmp-2 in Endometriosis 
Microrna?675?3p Promotes Esophageal Squamous Cell Cancer Cell Migration and Invasion 
Correlations of Serum Vegf and Mmp-2 Levels with Clm in Crc Patients and Effects of Tace on Their Expressions 
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