EK1M02

Human MMP-2 ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:MMP2, MMP-2, TBE-1
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:10 μL
  • 灵敏度:69.14 pg/mL
  • 检测范围:2 ng/mL - 128 ng/mL
  • 回收率:86% - 118%

在售SKU:70-EK1M02-48, 70-EK1M02-96


描述

商品名

Human MMP-2 ELISA Kit (人基质金属蛋白酶2 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.1% - 7.8%;板间变异系数:2.4% - 3.8%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

10 μL

灵敏度

69.14 pg/mL

检测范围

2 ng/mL - 128 ng/mL

回收率

86% - 118%

平均回收率

1.08

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗MMP-2单克隆抗体的96孔聚苯乙烯酶标板
  • 人MMP-2冻干标准品
  • MMP-2检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人MMP-2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的MMP-2与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中MMP-2的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human MMP-2 ELISA Kit (人基质金属蛋白酶2 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 MMP2, MMP-2, TBE-1 靶点信息

MMP2 分子靶点信息概述

  • 分子名:MMP2, matrix metallopeptidase 2
  • 基因家族:M10 matrix metallopeptidases
  • 别名:TBE-1
  • 曾用名:CLG4; CLG4A
  • 全称:matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)

MMP2 分子靶点综述

基质金属蛋白酶2(MMP-2),又名72kDa的IV型胶原酶和明胶酶A,在人类中由MMP2基因编码。激活MMP-2需要蛋白水解加工。MMP-2在子宫内膜破裂、调节血管形成和炎症应答中起作用。MMP-2参与许多事件,如癌症进程、癌细胞浸润、细胞信号转导、新血管形成和淋巴管生成。MMP2基因突变与Torg-Winchester综合征、多发性骨溶解、关节炎综合征有关,可能与瘢痕疙瘩有关。非囊性纤维化支气管扩张中,相比于呼吸道感染铜绿假单胞菌的患者,感染流感嗜血杆菌患者的MMP-2浓度是升高的。

人 Human MMP2 分子靶点信息

  • 分子名:MMP2, matrix metallopeptidase 2
  • 别称:
    • 72 kDa gelatinase
    • 72 kDa type IV collagenase
    • CLG4
    • CLG4A
    • collagenase type IV-A
    • gelatinase A
    • matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
    • matrix metalloproteinase 2 (gelatinase a, 72kda gelatinase, 72kda type iv collagenase)
    • matrix metalloproteinase-2
    • matrix metalloproteinase-II
    • MMP-2
    • MMP-II
    • MONA
    • neutrophil gelatinase
    • TBE-1
  • 基因序列:NCBI_Gene: 4313
  • 蛋白序列:UniProtKB: P08253

人 Human MMP2靶点分子功能(预测)

Enables metallopeptidase activity and serine-type endopeptidase activity. Involved in several processes, including cellular response to UV-A; endodermal cell differentiation; and positive regulation of vascular associated smooth muscle cell proliferation. Located in extracellular space. Colocalizes with collagen-containing extracellular matrix. Implicated in artery disease (multiple); eye disease (multiple); pseudoxanthoma elasticum; and temporal arteritis. Biomarker of several diseases, including artery disease (multiple); carcinoma (multiple); cardiomyopathy (multiple); cholesteatoma (multiple); and neurodegenerative disease (multiple).

操作步骤

ELISA操作常见问题

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Curcumin suppresses the proliferation and tumorigenicity of Cal27 by modulating cancer-associated fibroblasts of TSCC 

  2. MicroRNA‑675‑3p promotes esophageal squamous cell cancer cell migration and invasion 

  3. Odd-skipped related 1 plays a tumor suppressor role in ovarian cancer via promoting follistatin-like protein 1 transcription 

  4. Rab11a promotes the malignant progression of ovarian cancer by inducing autophagy 

  5. Rhizoma Paridis saponins suppresses vasculogenic mimicry formation and metastasis in osteosarcoma through regulating miR-520d-3p/MIG-7 axis 

  6. Expression and significance of autophagy genes LC3, Beclin1 and MMP-2 in endometriosis 

  7. Integration of Dual Targeting and Dual Therapeutic Modules Endows Self-Assembled Nanoparticles with Anti-Tumor Growth and Metastasis Functions 

  8. Baicalein inhibits inflammatory response and promotes osteogenic activity in periodontal ligament cells challenged with lipopolysaccharides 

  9. Effects of β2/aβ2 on oxLDL-induced CD36 activation in THP-1 macrophages 

  10. Lin28/microRNA-let-7a promotes metastasis under circumstances of hyperactive Wnt signaling in esophageal squamous cell carcinoma 

  11. Effect of Orthodontic Combined with Implant Repair on Aesthetic Effect and Gingival Crevicular Fluid Factor in Patients with Dentition Defect and Periodontitis 

  12. N-acetylgalactosaminyltransferase GALNT6 is a potential therapeutic target of clear cell renal cell carcinoma progression 

  13. Correlation between matrix metalloproteinase-2, matrix metalloproteinase-9, and tissue inhibitor of metalloproteinases-1 and white matter hyperintensities in patients with cerebral small vessel disease based on cranial magnetic resonance 3D imaging 

  14. NR2F1 overexpression alleviates trophoblast cell dysfunction by inhibiting GDF15/MAPK axis in preeclampsia 

  15. New link between RNH1 and E2F1: regulates the development of lung adenocarcinoma 

  16. PELI1 overexpression contributes to pancreatic cancer progression through upregulating ubiquitination-mediated INPP5J degradation 

  17. Knockdown of Galectin-9 alleviates rheumatoid arthritis through suppressing TNF-α-induced activation of fibroblast-like synoviocytes 

  18. Kallikrein-Related Peptidase 4 Promotes Proliferation, Migration, Invasion, and Pro-Angiogenesis of Endometrial Stromal Cells via Regulation of Brain-Derived Neurotrophic Factor Production in Endometriosis 

  19. Inhibition of LAMP3 mediates the protective effect of vitamin D against hypoxia/reoxygenation in trophoblast cells 

  20. Oncogenic Role of the NFATC2/NEDD4/FBP1 Axis in Cholangiocarcinoma 

  21. Expression and Significance of Autophagy Genes Lc3, Beclin1 and Mmp-2 in Endometriosis 

  22. Microrna?675?3p Promotes Esophageal Squamous Cell Cancer Cell Migration and Invasion 

  23. Correlations of Serum Vegf and Mmp-2 Levels with Clm in Crc Patients and Effects of Tace on Their Expressions 

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