Human MMP-13 Standard (人基质金属蛋白酶13 (胶原酶3) 标准品)
¥280.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
描述
文章目录[隐藏]
本产品只包含标准品试剂,如需购买试剂盒请点击下图
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- EK1M13
- ELISA试剂盒
Human MMP-13 ELISA Kit检测试剂盒(酶联免疫吸附法)
- ¥1,600.00 – ¥2,650.00
商品名 |
Human MMP-13 Standard (人基质金属蛋白酶13 (胶原酶3) 标准品) |
---|---|
组分 |
人基质金属蛋白酶13 (MMP-13胶原酶3) 标准品 |
检测方法 |
双抗夹心法 |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
板式 |
管 |
保存 |
短期4℃,长期-20℃保存 |
运输条件 |
4℃蓝冰运输 |
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人CD28预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和辣根过氧化物酶标记的检测抗体,经过孵育,样本中存在的CD28与固相抗体和检测抗体结合。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中CD28的浓度成正比。加入终止液终止反应,在450 nm波长 (参考波长570 - 630 nm)测定吸光度值。
分子信息
MMP13 分子靶点信息概述
- 分子名:MMP13, matrix metallopeptidase 13
- 基因家族:Receptor ligands; M10 matrix metallopeptidases
- 别名:CLG3
- 全称:collagenase 3; matrix metalloproteinase 13 (collagenase 3)
MMP13 分子靶点综述
基质金属蛋白酶(MMPs)是钙依赖性的含锌蛋白酶,其中有 26 个成员(MMP-1 至 MMP-26)已被确定。MMPs 参与正常生理过程(如胚胎发育、繁殖、组织重塑)和病理过程(如关节炎和转移)中细胞外基质的降解。MMP-13,又名胶原酶 3,在人类中由 MMP13 基因编码。与大多数 MMPs 一样,它以非活性的前体形式被分泌存在。前肽结构域一旦被裂解,MMP-13 就被激活,该活化酶由催化结构域和类血红素结构域 PDB: 1PEX 组成。在胚胎发育过程中,MMP-13 在骨骼中表达,对于骨矿化中胶原基质的重组是必不可少的。在病理情况下,如肿瘤、类风湿关节炎和骨关节炎,MMP-13 是高度过表达的。
人 Human MMP13 分子靶点信息
- 分子名:MMP13, matrix metallopeptidase 13
- 别称:
- CLG3
- collagenase 3
- MANDP1
- matrix metallopeptidase 13 (collagenase 3)
- matrix metalloproteinase 13 (collagenase 3)
- MDST
- MMP-13
- 基因序列:NCBI_Gene: 4322
- 蛋白序列:UniProtKB: P45452
人 Human MMP13靶点分子功能(预测)
Enables collagen binding activity; metal ion binding activity; and metalloendopeptidase activity. Involved in bone morphogenesis; collagen catabolic process; and extracellular matrix disassembly. Predicted to be located in extracellular space. Predicted to be part of collagen trimer. Implicated in anodontia; artery disease (multiple); osteoarthritis; ovarian carcinoma; and spondyloepimetaphyseal dysplasia, Missouri type. Biomarker of invasive ductal carcinoma; reproductive organ cancer (multiple); and urinary bladder cancer.
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 血清OR血浆,哪个是ELISA的菜 
- ELISA通关必备丨数据篇丨标准曲线不佳 
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- ELISA通关必备丨操作篇丨溶解与稀释标准品 
- ELISA通关必备丨样本篇丨不常见样本 
- ELISA通关必备丨样本篇丨常见样本丨细胞 
- ELISA通关必备丨如何选择试剂盒 
- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
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- 查看更多ELISA操作相关问题
ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- Elevated microRNA‑145‑5p increases matrix metalloproteinase‑9 by activating the nuclear factor‑κB pathway in rheumatoid arthritis 
- Insulin Exacerbates Inflammation in Fibroblast-Like Synoviocytes 
- Pectolinarigenin targeting FGFR3 alleviates osteoarthritis progression by regulating the NF-κB/NLRP3 inflammasome pyroptotic pathway 
- Sulfasalazine promotes ferroptosis through AKT-ERK1/2 and P53-SLC7A11 in rheumatoid arthritis 
- Knockdown of Galectin-9 alleviates rheumatoid arthritis through suppressing TNF-α-induced activation of fibroblast-like synoviocytes 
- Bone Mesenchymal Stem Cell-derived Exosomes Involved Co-delivery and Synergism Effect with Icariin via Mussel-inspired Multifunctional Hydrogel for Cartilage Protection 
- IL-37 alleviates intervertebral disc degeneration via the IL-1R8/NF-κB pathway 
Elevated microRNA‑145‑5p increases matrix metalloproteinase‑9 by activating the nuclear factor‑κB pathway in rheumatoid arthritis 
Insulin Exacerbates Inflammation in Fibroblast-Like Synoviocytes 
Pectolinarigenin targeting FGFR3 alleviates osteoarthritis progression by regulating the NF-κB/NLRP3 inflammasome pyroptotic pathway 
Sulfasalazine promotes ferroptosis through AKT-ERK1/2 and P53-SLC7A11 in rheumatoid arthritis 
Knockdown of Galectin-9 alleviates rheumatoid arthritis through suppressing TNF-α-induced activation of fibroblast-like synoviocytes 
Bone Mesenchymal Stem Cell-derived Exosomes Involved Co-delivery and Synergism Effect with Icariin via Mussel-inspired Multifunctional Hydrogel for Cartilage Protection 
IL-37 alleviates intervertebral disc degeneration via the IL-1R8/NF-κB pathway 
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