Human MIF ELISA Kit 检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:MIF, GLIF
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:100 μL
- 灵敏度:1.00 pg/mL
- 检测范围:31.25 pg/mL - 2000 pg/mL
- 回收率:95% - 112%
在售SKU:70-EK1158-48, 70-EK1158-96
描述
商品名 |
Human MIF ELISA Kit (人巨噬细胞游走抑制因子 (MIF) ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:2.0% - 4.0%;板间变异系数:2.0% - 4.3% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
100 μL |
灵敏度 |
1.00 pg/mL |
检测范围 |
31.25 pg/mL - 2000 pg/mL |
回收率 |
95% - 112% |
平均回收率 |
1.05 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人MIF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的MIF与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中MIF的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
MIF 分子靶点信息概述
- 分子名:MIF, macrophage migration inhibitory factor
- 别名:GIF
- 曾用名:GLIF
- 全称:glycosylation-inhibiting factor; phenylpyruvate tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor)
MIF 分子靶点综述
巨噬细胞游走抑制因子(MIF)是先天免疫的重要调节子。细菌抗原刺激白细胞释放MIF到血流中。循环的MIF结合其它免疫细胞上的CD74进而引发急性免疫反应,因此MIF是一种炎性细胞因子。此外,糖皮质激素也可刺激白细胞释放MIF。MIF通过抑制糖皮质激素的消炎效果来调节巨噬细胞在宿主防御中的功能。MIF是一种与类风湿关节炎严重程度相关的炎性介质。精神分裂症患者血浆中的MIF蛋白水平会升高,包括在疾病的早期阶段,但是这种现象的临床意义还未确定。MIF同样可诱导脓毒症过程中的心脏变化。另外,有证据表明,MIF产量和结肠癌的转移潜能之间存在一定关联。
人 Human MIF 分子靶点信息
- 分子名:MIF, macrophage migration inhibitory factor
- 别称:
- GIF
- GLIF
- L-dopachrome isomerase
- L-dopachrome tautomerase
- macrophage migration inhibitory factor (glycosylation-inhibiting factor)
- MMIF
- phenylpyruvate tautomerase
- 基因序列:NCBI_Gene: 4282
- 蛋白序列:UniProtKB: P14174
人 Human MIF靶点分子功能(预测)
Enables several functions, including identical protein binding activity; intramolecular oxidoreductase activity; and receptor ligand activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of protein phosphorylation; and regulation of intracellular signal transduction. Located in several cellular components, including cell surface; extracellular space; and nucleoplasm. Implicated in allergic disease; asthma; cystic fibrosis; lung disease (multiple); and obesity. Biomarker of artery disease (multiple); asthma; lung disease (multiple); obesity; and rhinitis.
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 血清OR血浆,哪个是ELISA的菜 
- ELISA通关必备丨数据篇丨标准曲线不佳 
- ELISA通关必备丨操作篇丨常见问题及解决方案 
- ELISA通关必备丨操作篇丨溶解与稀释标准品 
- ELISA通关必备丨样本篇丨不常见样本 
- ELISA通关必备丨样本篇丨常见样本丨细胞 
- ELISA通关必备丨如何选择试剂盒 
- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
- ELISA常见类型一 | 双抗夹心法,你要的都在这里! 
- ELISA常见类型二 | 竞争法,五分钟搞定! 
- 叮!联科向您投递了个ELISA实验操作干货包,请查收~ 
- 【视频】ELISA实验操作步骤演示视频教程 
- ELISA 组织样本的处理—大鼠组织 
- 【视频】ELISA实验原理与常见问题分析 
- 查看更多ELISA操作相关问题
ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- p-Hydroxylcinnamaldehyde induces the differentiation of oesophageal carcinoma cells via the cAMP-RhoA-MAPK signalling pathway 
- Macrophage migration inhibitory factor derived from spinal cord is involved in activation of macrophages following gecko tail amputation 
- The role of serum macrophage migration inhibitory factor in preoperative prediction of chronic rhinosinusitis with nasal polyps endotypes 
- Circulating MIF Associated With Disease Severity and Clinical Response of Sublingual Immunotherapy in House Dust Mite–Induced Allergic Rhinitis 
- Identification of Robust Biomarkers for Early Predicting Efficacy of Subcutaneous Immunotherapy in Children With House Dust Mite-Induced Allergic Rhinitis by Multiple Cytokine Profiling 
- Cisplatin-stimulated macrophages promote ovarian cancer migration via the CCL20-CCR6 axis 
- MAPK4 silencing in gastric cancer drives liver metastasis by positive feedback between cancer cells and macrophages 
p-Hydroxylcinnamaldehyde induces the differentiation of oesophageal carcinoma cells via the cAMP-RhoA-MAPK signalling pathway 
Macrophage migration inhibitory factor derived from spinal cord is involved in activation of macrophages following gecko tail amputation 
The role of serum macrophage migration inhibitory factor in preoperative prediction of chronic rhinosinusitis with nasal polyps endotypes 
Circulating MIF Associated With Disease Severity and Clinical Response of Sublingual Immunotherapy in House Dust Mite–Induced Allergic Rhinitis 
Identification of Robust Biomarkers for Early Predicting Efficacy of Subcutaneous Immunotherapy in Children With House Dust Mite-Induced Allergic Rhinitis by Multiple Cytokine Profiling 
Cisplatin-stimulated macrophages promote ovarian cancer migration via the CCL20-CCR6 axis 
MAPK4 silencing in gastric cancer drives liver metastasis by positive feedback between cancer cells and macrophages 
评价
目前还没有评价