Human Lipocalin-2/NGAL ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:LCN2, NGAL
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:100 μL
- 灵敏度:2.42 pg/mL
- 检测范围:125 pg/mL - 8000 pg/mL
- 回收率:84% - 107%
在售SKU:70-EK1116-48, 70-EK1116-96
描述
商品名 |
Human Lipocalin-2/NGAL ELISA Kit (人脂质运载蛋白2 ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:2.0% - 4.5%;板间变异系数:2.9% - 5.0% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
100 μL |
灵敏度 |
2.42 pg/mL |
检测范围 |
125 pg/mL - 8000 pg/mL |
回收率 |
84% - 107% |
平均回收率 |
0.98 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人Lipocalin-2抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的Lipocalin-2与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中Lipocalin-2的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
LCN2 分子靶点信息概述
- 分子名:LCN2, lipocalin 2
- 基因家族:Lipocalins
- 别名:NGAL; 24p3
- 全称:oncogene 24p3; neutrophil gelatinase-associated lipocalin; siderocalin; lipocalin 2 (oncogene 24p3)
LCN2 分子靶点综述
脂质运载蛋白2,又名中性粒细胞明胶酶相关的脂质运载蛋白(NGAL)或癌基因24p3,在中性粒细胞中表达,在肾脏、前列腺、呼吸道和消化道的上皮细胞中低表达。Lipocalin-2通过螯合铁来参与先天性免疫,反过来可限制细菌的生长。Lipocalin-2是肾损伤的生物标志物,还可作为慢性肾脏病、造影剂肾病和肾移植的早期诊断物。在急性肾损伤(AKI)的2小时内,高水平的Lipocalin-2被分泌到血液和尿中。机体的Lipocalin-2水平与AKI的严重程度成正比。
人 Human LCN2 分子靶点信息
- 分子名:LCN2, lipocalin 2
- 别称:
- 24p3
- 25 kDa alpha-2-microglobulin-related subunit of MMP-9
- lipocalin-2
- migration-stimulating factor inhibitor
- MSFI
- neutrophil gelatinase-associated lipocalin
- NGAL
- oncogene 24p3
- p25
- siderocalin
- siderocalin LCN2
- 基因序列:NCBI_Gene: 3934
- 蛋白序列:UniProtKB: P80188
人 Human LCN2靶点分子功能(预测)
Enables enterobactin binding activity; identical protein binding activity; and iron ion binding activity. Involved in defense response to bacterium and sequestering of iron ion. Located in extracellular space. Implicated in urinary tract infection. Biomarker of several diseases, including congenital diaphragmatic hernia; hidradenitis suppurativa; hydronephrosis; hypertension (multiple); and kidney disease (multiple).
操作步骤
文章目录[隐藏]
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引用文献
文章目录[隐藏]
- lncRNA MALAT1 mediated high glucose–induced HK-2 cell epithelial-to-mesenchymal transition and injury 
- Platelet-derived extracellular vesicles are associated with kidney injury in patients with urosepsis 
- Assessment of osteopontin as an early nephrotoxicity indicator in human renal proximal tubule cells and its application in evaluating lanthanum-induced nephrotoxicity 
- A biomimetic liver cancer on-a-chip reveals a critical role of LIPOCALIN-2 in promoting hepatocellular carcinoma progression 
lncRNA MALAT1 mediated high glucose–induced HK-2 cell epithelial-to-mesenchymal transition and injury 
Platelet-derived extracellular vesicles are associated with kidney injury in patients with urosepsis 
Assessment of osteopontin as an early nephrotoxicity indicator in human renal proximal tubule cells and its application in evaluating lanthanum-induced nephrotoxicity 
A biomimetic liver cancer on-a-chip reveals a critical role of LIPOCALIN-2 in promoting hepatocellular carcinoma progression 
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