EK108

Human IL-8 ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥10,800.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：CXCL8, IL-8, GCP1, NAP1
  • 种属：人 (Human)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：50 μL
  • 灵敏度：0.78 pg/mL
  • 检测范围：15.63 pg/mL - 1000 pg/mL
  • 回收率：87% - 111%

在售SKU:70-EK108-48, 70-EK108-96, 人白细胞介素8, 白细胞介素8, 白细胞介素, 人, 白介素, 8

描述

商品名

Human IL-8 ELISA Kit (人白介素8 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：4.0% - 4.9%；板间变异系数：4.5% - 6.5%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

50 μL
灵敏度

0.78 pg/mL
检测范围

15.63 pg/mL - 1000 pg/mL
回收率

87% - 111%
平均回收率

0.99
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IL-8单克隆抗体的96孔聚苯乙烯酶标板
  • 人IL-8冻干标准品
  • IL-8检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-8抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-8与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-8的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IL-8 ELISA Kit (人白介素8 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 CXCL8, IL-8, GCP1, NAP1 靶点信息

CXCL8 分子靶点信息概述

  • 分子名：CXCL8, C-X-C motif chemokine ligand 8
  • 基因家族：Chemokine ligands; Interleukins
  • 别名：SCYB8; LUCT; LECT; MDNCF; TSG-1; IL-8; NAP-1; 3-10C; MONAP; AMCF-I; LYNAP; NAF; b-ENAP; GCP-1; K60; GCP1; NAP1
  • 曾用名：IL8
  • 全称：neutrophil-activating peptide 1; granulocyte chemotactic protein 1; monocyte-derived neutrophil chemotactic factor; lung giant cell carcinoma-derived chemotactic protein; tumor necrosis factor-induced gene 1; monocyte-derived neutrophil-activating peptide; lymphocyte derived neutrophil activating peptide; beta endothelial cell-derived neutrophil activating peptide; alveolar macrophage chemotactic factor I; interleukin 8; chemokine (C-X-C motif) ligand 8

CXCL8 分子靶点综述

白细胞介素8(IL-8)是由巨噬细胞和其它类型细胞如上皮细胞、气道平滑肌细胞和内皮细胞产生的趋化因子，在人类中，由IL8基因编码。IL-8可有效促进血管生成，在细支气管炎的发病机制中起重要作用。IL-8可通过对中性粒细胞的召集和脱颗粒来实现对炎症反应的调节，可作为结肠癌细胞株的自分泌生长因子来作用于结肠直肠癌。高水平的IL-8可减少精神分裂症患者抗精神病药的阳性反应。另外，IL-8与肥胖和囊性纤维化的发病机制也相关。

人 Human CXCL8 分子靶点信息

  • 分子名：CXCL8, C-X-C motif chemokine ligand 8
  • 别称：
    • alveolar macrophage chemotactic factor I
    • beta endothelial cell-derived neutrophil activating peptide
    • beta-thromboglobulin-like protein
    • C-X-C motif chemokine 8
    • chemokine (C-X-C motif) ligand 8
    • emoctakin
    • GCP-1
    • GCP1
    • granulocyte chemotactic protein 1
    • IL8
    • interleukin 8
    • interleukin-8
    • LECT
    • LUCT
    • lung giant cell carcinoma-derived chemotactic protein
    • lymphocyte derived neutrophil activating peptide
    • lymphocyte-derived neutrophil-activating factor
    • LYNAP
    • MDNCF
    • MONAP
    • monocyte-derived neutrophil chemotactic factor
    • monocyte-derived neutrophil-activating peptide
    • NAF
    • NAP-1
    • NAP1
    • neutrophil-activating peptide 1
    • SCYB8
    • small inducible cytokine subfamily B, member 8
    • T cell chemotactic factor
    • T-cell chemotactic factor
    • tumor necrosis factor-induced gene 1
  • 基因序列：NCBI_Gene: 3576
  • 蛋白序列：UniProtKB: P10145

人 Human CXCL8靶点分子功能（预测）

Enables chemokine activity and interleukin-8 receptor binding activity. Involved in several processes, including cellular response to cytokine stimulus; induction of positive chemotaxis; and neutrophil chemotaxis. Acts upstream of or within negative regulation of cell adhesion molecule production; positive regulation of cellular biosynthetic process; and regulation of gene expression. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Implicated in several diseases, including autoimmune uveitis; bacterial gastritis; gastrointestinal system cancer (multiple); trachoma; and urinary tract infection. Biomarker of several diseases, including chikungunya; cholestasis (multiple); cystic fibrosis; liver disease (multiple); and tuberculosis (multiple).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Tumor associated neutrophils promote the metastasis of pancreatic ductal adenocarcinoma 

  2. The role of alcohol dehydrogenase 1C in regulating inflammatory responses in ulcerative colitis 

  3. P38 MAPK, NF-κB, and JAK-STAT3 Signaling Pathways Involved in Capecitabine-Induced Hand-Foot Syndrome via Interleukin 6 or Interleukin 8 Abnormal Expression 

  4. Short-term efficacy, safety and survival of laparoscopic radical resection for colorectal cancer patients with bowel obstruction 

  5. Galectin-3 exacerbates ox-LDL-mediated endothelial injury by inducing inflammation via integrin β1-RhoA-JNK signaling activation 

  6. Inflammatory Cytokines Changed in Patients With Depression Before and After Repetitive Transcranial Magnetic Stimulation Treatment 

  7. Helicobacter pylori tumor necrosis factor-α inducing protein promotes cytokine expression via nuclear factor-κB 

  8. Fusobacterium nucleatum stimulates monocyte adhesion to and transmigration through endothelial cells 

  9. Ex vivo lung perfusion with perfusate purification for human donor lungs following prolonged cold storage 

  10. A cross-sectional study reveals a chronic low-grade inflammation in achalasia 

  11. Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 

  12. CD4+/CD8+ mucosa-associated invariant T cells foster the development of endometriosis: a pilot study 

  13. Alteration in the Cytokine Secretion of Bone Marrow Stromal Cells from Patients with Chronic Myelomonocytic Leukemia Contribute to Impaired Hematopoietic Supportive Activity 

  14. Cancer-associated adipocytes promote the invasion and metastasis in breast cancer through LIF/CXCLs positive feedback loop 

  15. Adhesion-Related Immunomodulatory Activity of the Screened Lactobacillus plantarum from Sichuan Pickle 

  16. Erastin enhances metastatic potential of ferroptosis-resistant ovarian cancer cells by M2 polarization through STAT3/IL-8 axis 

  17. C. butyricum lipoteichoic acid inhibits the inflammatory response and apoptosis in HT-29 cells induced by S. aureus lipoteichoic acid 

  18. Network pharmacology-based prediction and verification of the active ingredients and potential targets of Huagan Decoction for reflux esophagitis 

  19. Oral delivery of Bifidobacterium longum expressing α-melanocyte-stimulating hormone to combat ulcerative colitis 

  20. In vitro evaluation of probiotic potential of Pediococcus pentosaceus L1 isolated from paocai—a Chinese fermented vegetable 

  21. Fenofibrate, a PPARα agonist, protect proximal tubular cells from albumin-bound fatty acids induced apoptosis via the activation of NF-kB 

  22. Changes in peripheral blood cytokines in patients with severe fever with thrombocytopenia syndrome 

  23. Antimicrobial activities of peptide Cbf-K16 against drug-resistant Helicobacter pylori infection in vitro and in vivo 

  24. Antinuclear antibodies in healthy population: Positive association with abnormal tissue metabolism, inflammation and immune dysfunction 

  25. RBM23 Drives Hepatocellular Carcinoma by Activating NF-κB Signaling Pathway 

  26. SOMG-833, a Novel Selective c-MET Inhibitor, Blocks c-MET–Dependent Neoplastic Effects and Exerts Antitumor Activity 

  27. Comprehensive Analysis of NAFLD and the Therapeutic Target Identified 

  28. Diallyl trisulfide inhibits naphthalene-induced oxidative injury and the production of inflammatory responses in A549 cells and mice 

  29. Different effects of lipoteichoic acid from C. butyricum and S. aureus on inflammatory responses of HT-29 cells 

  30. TLR2 expression doesn’t change in ox-LDL mediated inflammation in Human umbilical vein endothelial cells under high glucose culture 

  31. Participation of Increased Circulating MAIT Cells in Lung Cancer: a Pilot Study 

  32. The effect of liraglutide on the proliferation, migration, and osteogenic differentiation of human periodontal ligament cells 

  33. Kynureninase contributes to the pathogenesis of psoriasis through pro-inflammatory effect 

  34. Dimethyl fumarate protects nucleus pulposus cells from inflammation and oxidative stress and delays the intervertebral disc degeneration 

  35. HIF-1α/JMJD1A signaling regulates inflammation and oxidative stress following hyperglycemia and hypoxia-induced vascular cell injury 

  36. KMT2C Induced by FABP5P3 Aggravates Keratinocyte Hyperproliferation and Psoriasiform Skin Inflammation by Upregulating the Transcription of PIK3R3 

  37. Berberine Protects against TNF-α-Induced Injury of Human Umbilical Vein Endothelial Cells via the AMPK/NF-κB/YY1 Signaling Pathway 

  38. Elevated latent transforming growth factor beta binding protein 2 in endometriosis promotes endometrial stromal cell invasion and proliferation via the NF-kB signaling pathway 

  39. RIPK3-Dependent Necroptosis Is Induced and Restricts Viral Replication in Human Astrocytes Infected With Zika Virus 

  40. Hydrofluoric acid and nitric acid cotreatment for biofunctionalization of polyetheretherketone in M2 macrophage polarization and osteogenesis 

  41. The clinical study on treatment of CD19-directed chimeric antigen receptor-modified T cells in a case of refractory Richter syndrome 

  42. Oncolytic Vaccinia Virus Carrying Aphrocallistes vastus Lectin (oncoVV-AVL) Enhances Inflammatory Response in Hepatocellular Carcinoma Cells 

  43. Simvastatin Reduces Myocardial Injury Undergoing Noncoronary Artery Cardiac Surgery 

  44. miR-141-3p Regulates EZH2 to Attenuate Porphyromonas gingivalis Lipopolysaccharide-Caused Inflammation and Inhibition of Osteogenic Differentiation in Human Periodontal Ligament Stem Cells 

  45. MiR-155-5p modulates inflammatory phenotype of activated oral lichen-planus-associated-fibroblasts by targeting SOCS1 

  46. Bergenin-activated SIRT1 inhibits TNF-α-induced proinflammatory response by blocking the NF-κB signaling pathway 

  47. Ultrasound-Guided Percutaneous Transhepatic Gallbladder Drainage Improves the Prognosis of Patients with Severe Acute Cholecystitis 

  48. A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide. 

  49. Effects of intranasal administration with Bacillus subtilis on immune cells in the nasal mucosa and tonsils of piglets 

  50. Effects of dietary fat on gut microbiota and faecal metabolites, and their relationship with cardiometabolic risk factors: a 6-month randomised controlled-feeding trial 

  51. IgD-activated mast cells induce IgE synthesis in B cells in nasal polyps 

  52. Canonical and noncanonical pyroptosis are both activated in periodontal inflammation and bone resorption 

  53. Icariin arrests cell cycle progression and induces cell apoptosis through the mitochondrial pathway in human fibroblast-like synoviocytes 

  54. E3 Ubiquitin ligase RNF183 Is a Novel Regulator in Inflammatory Bowel Disease 

  55. Expression of RANKL by peripheral neutrophils and its association with bone mineral density in COPD 

  56. Lactate Transporter SLC16A3 (MCT4) as an Onco-Immunological Biomarker Associating Tumor Microenvironment and Immune Responses in Lung Cancer 

  57. Characterization of the release and biological significance of cell-free DNA from breast cancer cell lines 

  58. LncRNA lnc-SPRR2G-2 contributes to keratinocyte hyperproliferation and inflammation in psoriasis by activating the STAT3 pathway and downregulating KHSRP 

  59. Coffee leaf polyphenol-rich extracts alleviated lipopolysaccharide-induced intestinal barrier dysfunction: Insights from a Caco-2/U937 co-culture model 

  60. Macrophage Extracellular Traps Suppress Particulate Matter–Induced Airway Inflammation 

  61. The discovery of a novel pyrrolo[2,3-b]pyridine as a selective CDK8 inhibitor offers a new approach against psoriasis 

  62. Procyanidin B2 alleviates oxidized low-density lipoprotein-induced cell injury, inflammation, monocyte chemotaxis, and oxidative stress by inhibiting the nuclear factor kappa-B pathway in human umbilical vein endothelial cells 

  63. Targeting osteopontin alleviates endometriosis and inflammation by inhibiting the RhoA/ROS axis and achieves non-invasive in vitro detection via menstrual blood 

  64. High Expression of miR-6785-5p in the Serum Exosomes of Psoriasis Patients Alleviates Psoriasis-Like Skin Damage by Interfering with the MNK2/p-eIF4E Axis in Keratinocytes 

  65. CircACTR2 attenuated the effects of tetramethylpyrazine on human kidney cell injury 

  66. Synthetic steroid of 5α-Androst-3β,5α,6β-Triol alleviates acute lung injury via inhibiting inflammation and oxidative stress 

  67. FSTL1 Accelerates Nucleus Pulposus Cell Senescence and Intervertebral Disc Degeneration Through TLR4/NF-κB Pathway 

  68. Analysis of intestinal flora in elderly Uygur patients with sarcopenia 

  69. Formononetin attenuates cigarette smoke-induced COPD in mice by suppressing inflammation, endoplasmic reticulum stress, and apoptosis in bronchial epithelial cells via AhR/CYP1A1 and AKT/mTOR signaling pathways 

  70. Knockdown of Galectin-9 alleviates rheumatoid arthritis through suppressing TNF-α-induced activation of fibroblast-like synoviocytes 

  71. Airway epithelial cGAS inhibits LPS-induced acute lung injury through CREB signaling 

  72. BMI-specific inflammatory response to phthalate exposure in early pregnancy: findings from the TMCHESC study 

  73. Serine deficiency exacerbates psoriatic skin inflammation by regulating S-adenosyl methionine-dependent DNA methylation and NF-κB signaling activation in keratinocytes 

  74. SMAD2 inhibits pyroptosis of fibroblast-like synoviocytes and secretion of inflammatory factors via the TGF-β pathway in rheumatoid arthritis 

  75. An inflammatory checkpoint generated by IL1RN splicing offers therapeutic opportunity for KRAS mutant intrahepatic cholangiocarcinoma 

  76. Potential salivary and serum biomarkers for burning mouth syndrome and their relationship with anxiety/depression 

  77. Discovery of a novel RIPK2 inhibitor for the treatment of inflammatory bowel disease 

  78. Polygonatum kingianum saponins delay cellular senescence through SASP down-regulation and prolong the healthy lifespan of Caenorhabditis elegans by activating sir-2.1/autophagy 

  79. The Natural Alkaloid (?)-N-Hydroxyapiosporamide Suppresses Colorectal Tumor Progression as an NF-κB Pathway Inhibitor by Targeting the TAK1–TRAF6 Complex 

  80. Silibinin attenuated pseudo-allergic reactions and mast cell degranulation via PLCγ and PI3K/Akt signaling pathway 

  81. Decreased Sphingosine Due to Down-Regulation of Acid Ceramidase Expression in Airway of Bronchiectasis Patients: A Potential Contributor to Pseudomonas aeruginosa Infection 

  82. Multifunctional chondroitin sulfate based hydrogels for promoting infected diabetic wounds healing by chemo-photothermal antibacterial and cytokine modulation 

  83. Original research: Chordoma recruits and polarizes tumor-associated macrophages via secreting CCL5 to promote malignant progression 

  84. CSF2 upregulates CXCL3 expression in adipocytes to promote metastasis of breast cancer via the FAK signaling pathway 

  85. Profiling of metabolites, proteins, and protein phosphorylation in silica-exposed BEAS-2B epithelial cells 

  86. Epstein–Barr virus envelope glycoprotein 110 inhibits NF-κB activation by interacting with NF-κB subunit p65 

  87. Telotristat Etiprate alleviates rheumatoid arthritis by targeting LGALS3 and affecting MAPK signaling 

  88. Acteoside alleviates asthma by modulating ROS-responsive NF-κB/MAPK signaling pathway 

  89. Effect of fermentation modification on the physicochemical characteristics and anti-aging related activities of Polygonatum kingianum polysaccharides 

  90. 4-Iodo-6-phenylpyrimidine (4-IPP) suppresses fibroblast-like synoviocyte- mediated inflammation and joint destruction associated with rheumatoid arthritis 

  91. A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide 

  92. 血清

    Simvastatin Reduces Myocardial Injury Undergoing Noncoronary Artery Cardiac Surgery: A Randomized Controlled Trial 

  93. 腹水 血浆

    CD4+/CD8+ mucosa-associated invariant T cells foster the development of endometriosis: a pilot study 

  94. 腹腔上清液

    Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 

  95. 血清

    Participation of Increased Circulating MAIT Cells in Lung Cancer: a Pilot Study 

  96. 细胞培养上清

    Dedifferentiation-associated inflammatory factors of long-term expanded human hepatocytes exacerbate their elimination by macrophages during liver engraftment 

  97. Antimicrobial Activities of Peptide Cbf-K(16) against Drug-Resistant Helicobacter Pylori Infection in Vitro and in Vivo 

  98. C. Butyricum Lipoteichoic Acid Inhibits the Inflammatory Response and Apoptosis in Ht-29 Cells Induced by S. Aureus Lipoteichoic Acid 

  99. Oral Delivery of Bifidobacterium Longum Expressing Α-Melanocyte-Stimulating Hormone to Combat Ulcerative Colitis 

  100. Somg-833, a Novel Selective C-Met Inhibitor, Blocks C-Met-Dependent Neoplastic Effects and Exerts Antitumor Activity 

  101. Fenofibrate, a Pparα Agonist, Protect Proximal Tubular Cells from Albumin-Bound Fatty Acids Induced Apoptosis Via the Activation of Nf-Kb 

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