EK105

Human IL-5 ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:IL5, IL-5, TRF
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:50 μL
  • 灵敏度:0.76 pg/mL
  • 检测范围:7.81 pg/mL - 500 pg/mL
  • 回收率:91% - 107%

在售SKU:70-EK105-48, 70-EK105-96, 人白细胞介素5, 白细胞介素5, 白细胞介素, 人, 白介素, 5


描述

商品名

Human IL-5 ELISA Kit (人白介素5 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.5% - 4.0%;板间变异系数:3.2% - 6.1%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

50 μL

灵敏度

0.76 pg/mL

检测范围

7.81 pg/mL - 500 pg/mL

回收率

91% - 107%

平均回收率

0.99

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗IL-5单克隆抗体的96孔聚苯乙烯酶标板
  • 人IL-5冻干标准品
  • IL-5检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-5抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-5与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-5的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IL-5 ELISA Kit (人白介素5 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 IL5, IL-5, TRF 靶点信息

IL5 分子靶点信息概述

  • 分子名:IL5, interleukin 5
  • 基因家族:Interleukins
  • 别名:IL-5; EDF; TRF
  • 全称:interleukin-5; T-cell replacing factor; B cell differentiation factor I; eosinophil differentiation factor; colony-stimulating factor, eosinophil; interleukin 5 (colony-stimulating factor, eosinophil)

IL5 分子靶点综述

白细胞介素5(IL-5)是造血家族Th2细胞因子成员,由115个氨基酸组成。与该细胞因子家族其它成员(IL-3和GM-CSF)不同,该糖蛋白的活化形式是同源二聚体。IL-5由Th2细胞和肥大细胞分泌。它对B细胞以及其它类型的细胞产生的生物学活性表明,IL-5对Th2细胞的活性具有重要的功能性作用。IL-5通过与其受体结合,刺激B细胞生长,提高免疫球蛋白分泌。它也是活化嗜酸性粒细胞的关键因子。IL-5一直以来与多种变态疾病相关,包括变应性鼻炎和哮喘,在循环组织、气管组织中观察到大幅增加的IL-5,而痰嗜酸性粒细胞则减少。

人 Human IL5 分子靶点信息

  • 分子名:IL5, interleukin 5
  • 别称:
    • B cell differentiation factor I
    • B-cell differentiation factor I
    • colony-stimulating factor, eosinophil
    • EDF
    • eosinophil differentiation factor
    • IL-5
    • interleukin-5
    • T-cell replacing factor
    • TRF
  • 基因序列:NCBI_Gene: 3567
  • 蛋白序列:UniProtKB: P05113

人 Human IL5靶点分子功能(预测)

Predicted to enable cytokine activity; growth factor activity; and interleukin-5 receptor binding activity. Involved in positive regulation of podosome assembly. Located in extracellular region. Implicated in asthma. Biomarker of several diseases, including Churg-Strauss syndrome; Henoch-Schoenlein purpura; arthritis (multiple); asthma (multiple); and lymphoid leukemia (multiple).

操作步骤


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引用文献

文章目录[隐藏]


  1. Vitamin D deficiency is associated with thyroid autoimmunity: results from an epidemiological survey in Tianjin, China 

  2. Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 

  3. Type 2 innate lymphoid cells participate in IL‑33‑stimulated Th2‑associated immune response in chronic obstructive pulmonary disease 

  4. Berberine ameliorates mesenteric vascular dysfunction by modulating perivascular adipose tissue in diet-induced obese in rats 

  5. Downregulation of miRNA-451a Promotes the Differentiation of CD4+ T Cells towards Th2 Cells by Upregulating ETS1 in Childhood Asthma 

  6. ILC2 Cells Promote Th2 Cell Differentiation in AECOPD Through Activated Notch-GATA3 Signaling Pathway 

  7. ILC2s Induce Adaptive Th2-Type Immunity in Acute Exacerbation of Chronic Obstructive Pulmonary Disease 

  8. IgD-activated mast cells induce IgE synthesis in B cells in nasal polyps 

  9. Time‑series clustering of cytokine expression after transarterial chemoembolization in patients with hepatocellular carcinoma 

  10. c-CBL/LCK/c-JUN/ETS1/CD28 axis restrains childhood asthma by suppressing Th2 differentiation 

  11. CLDN1 silencing suppresses the proliferation and migration of airway smooth muscle cells by modulating MMP14 

  12. Collagen Triple Helix Repeat Containing 1 Deficiency Protects Against Airway Remodeling and Inflammation in Asthma Models In Vivo and In Vitro 

  13. 腹腔上清液

    Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 

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