Human IL-5 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:IL5, IL-5, TRF
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:50 μL
- 灵敏度:0.76 pg/mL
- 检测范围:7.81 pg/mL - 500 pg/mL
- 回收率:91% - 107%
在售SKU:70-EK105-48, 70-EK105-96, 人白细胞介素5, 白细胞介素5, 白细胞介素, 人, 白介素, 5
描述
商品名 |
Human IL-5 ELISA Kit (人白介素5 ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:2.5% - 4.0%;板间变异系数:3.2% - 6.1% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
50 μL |
灵敏度 |
0.76 pg/mL |
检测范围 |
7.81 pg/mL - 500 pg/mL |
回收率 |
91% - 107% |
平均回收率 |
0.99 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-5抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IL-5与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IL-5的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
IL5 分子靶点信息概述
- 分子名:IL5, interleukin 5
- 基因家族:Interleukins
- 别名:IL-5; EDF; TRF
- 全称:interleukin-5; T-cell replacing factor; B cell differentiation factor I; eosinophil differentiation factor; colony-stimulating factor, eosinophil; interleukin 5 (colony-stimulating factor, eosinophil)
IL5 分子靶点综述
白细胞介素5(IL-5)是造血家族Th2细胞因子成员,由115个氨基酸组成。与该细胞因子家族其它成员(IL-3和GM-CSF)不同,该糖蛋白的活化形式是同源二聚体。IL-5由Th2细胞和肥大细胞分泌。它对B细胞以及其它类型的细胞产生的生物学活性表明,IL-5对Th2细胞的活性具有重要的功能性作用。IL-5通过与其受体结合,刺激B细胞生长,提高免疫球蛋白分泌。它也是活化嗜酸性粒细胞的关键因子。IL-5一直以来与多种变态疾病相关,包括变应性鼻炎和哮喘,在循环组织、气管组织中观察到大幅增加的IL-5,而痰嗜酸性粒细胞则减少。
人 Human IL5 分子靶点信息
- 分子名:IL5, interleukin 5
- 别称:
- B cell differentiation factor I
- B-cell differentiation factor I
- colony-stimulating factor, eosinophil
- EDF
- eosinophil differentiation factor
- IL-5
- interleukin-5
- T-cell replacing factor
- TRF
- 基因序列:NCBI_Gene: 3567
- 蛋白序列:UniProtKB: P05113
人 Human IL5靶点分子功能(预测)
Predicted to enable cytokine activity; growth factor activity; and interleukin-5 receptor binding activity. Involved in positive regulation of podosome assembly. Located in extracellular region. Implicated in asthma. Biomarker of several diseases, including Churg-Strauss syndrome; Henoch-Schoenlein purpura; arthritis (multiple); asthma (multiple); and lymphoid leukemia (multiple).
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
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引用文献
文章目录[隐藏]
- Vitamin D deficiency is associated with thyroid autoimmunity: results from an epidemiological survey in Tianjin, China 
- Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 
- Type 2 innate lymphoid cells participate in IL‑33‑stimulated Th2‑associated immune response in chronic obstructive pulmonary disease 
- Berberine ameliorates mesenteric vascular dysfunction by modulating perivascular adipose tissue in diet-induced obese in rats 
- Downregulation of miRNA-451a Promotes the Differentiation of CD4+ T Cells towards Th2 Cells by Upregulating ETS1 in Childhood Asthma 
- ILC2 Cells Promote Th2 Cell Differentiation in AECOPD Through Activated Notch-GATA3 Signaling Pathway 
- ILC2s Induce Adaptive Th2-Type Immunity in Acute Exacerbation of Chronic Obstructive Pulmonary Disease 
- IgD-activated mast cells induce IgE synthesis in B cells in nasal polyps 
- Time‑series clustering of cytokine expression after transarterial chemoembolization in patients with hepatocellular carcinoma 
- c-CBL/LCK/c-JUN/ETS1/CD28 axis restrains childhood asthma by suppressing Th2 differentiation 
- CLDN1 silencing suppresses the proliferation and migration of airway smooth muscle cells by modulating MMP14 
- Collagen Triple Helix Repeat Containing 1 Deficiency Protects Against Airway Remodeling and Inflammation in Asthma Models In Vivo and In Vitro 
- Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 
Vitamin D deficiency is associated with thyroid autoimmunity: results from an epidemiological survey in Tianjin, China 
Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 
Type 2 innate lymphoid cells participate in IL‑33‑stimulated Th2‑associated immune response in chronic obstructive pulmonary disease 
Berberine ameliorates mesenteric vascular dysfunction by modulating perivascular adipose tissue in diet-induced obese in rats 
Downregulation of miRNA-451a Promotes the Differentiation of CD4+ T Cells towards Th2 Cells by Upregulating ETS1 in Childhood Asthma 
ILC2 Cells Promote Th2 Cell Differentiation in AECOPD Through Activated Notch-GATA3 Signaling Pathway 
ILC2s Induce Adaptive Th2-Type Immunity in Acute Exacerbation of Chronic Obstructive Pulmonary Disease 
IgD-activated mast cells induce IgE synthesis in B cells in nasal polyps 
Time‑series clustering of cytokine expression after transarterial chemoembolization in patients with hepatocellular carcinoma 
c-CBL/LCK/c-JUN/ETS1/CD28 axis restrains childhood asthma by suppressing Th2 differentiation 
CLDN1 silencing suppresses the proliferation and migration of airway smooth muscle cells by modulating MMP14 
Collagen Triple Helix Repeat Containing 1 Deficiency Protects Against Airway Remodeling and Inflammation in Asthma Models In Vivo and In Vitro 
- 腹腔上清液
Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 
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