EK113

Human IL-13 ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：IL13, ALRH, BHR1
  • 种属：人 (Human)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：50 μL
  • 灵敏度：0.90 pg/mL
  • 检测范围：7.81 pg/mL - 500 pg/mL
  • 回收率：82% - 110%

在售SKU:70-EK113-48, 70-EK113-96

描述

商品名

Human IL-13 ELISA Kit (人白细胞介素13 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：1.7% - 2.0%；板间变异系数：2.2% - 4.1%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

50 μL
灵敏度

0.90 pg/mL
检测范围

7.81 pg/mL - 500 pg/mL
回收率

82% - 110%
平均回收率

0.98
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IL-13单克隆抗体的96孔聚苯乙烯酶标板
  • 人IL-13冻干标准品
  • IL-13检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IL-13抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-13与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-13的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IL-13 ELISA Kit (人白细胞介素13 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 IL13, ALRH, BHR1 靶点信息

IL13 分子靶点信息概述

  • 分子名：IL13, interleukin 13
  • 基因家族：Interleukins
  • 别名：P600; IL-13; ALRH; BHR1; MGC116786; MGC116788; MGC116789
  • 全称：allergic rhinitis; Bronchial hyperresponsiveness-1 (bronchial asthma)

IL13 分子靶点综述

白细胞介素13(IL-13)位于染色体5q31上，与IL-4具有30%的同源性和相似的结构。IL-13由许多细胞类型分泌产生，尤其是2型辅助性T细胞(Th2)。与密切相关的IL-4相似，IL-13对免疫细胞也具有多种功能。虽然IL-13主要与气道疾病的诱导相关，但是它还具有消炎特性。IL-13可诱导造血细胞的变化，但是这些效果与IL-4相比可能不那么重要。IL-13专门诱导寄生感染的器官的生理变化。它也可诱导过敏性肺部疾病的许多特征，包括气道高反应性、杯状细胞增殖和黏液高分泌，这些都会导致气道阻塞。此外，IL-13抑制原代培养衍生的巨噬细胞中HIV-1的复制，可作为体内单核细胞和巨噬细胞中抑制HIV感染的候选细胞因子。

人 Human IL13 分子靶点信息

  • 分子名：IL13, interleukin 13
  • 别称：
    • allergic rhinitis
    • ALRH
    • BHR1
    • Bronchial hyperresponsiveness-1 (bronchial asthma)
    • IL-13
    • interleukin-13
    • MGC116786
    • MGC116788
    • MGC116789
    • P600
  • 基因序列：NCBI_Gene: 3596
  • 蛋白序列：UniProtKB: P35225

人 Human IL13靶点分子功能（预测）

Predicted to enable cytokine activity and interleukin-13 receptor binding activity. Involved in several processes, including negative regulation of complement-dependent cytotoxicity; negative regulation of endothelial cell apoptotic process; and positive regulation of interleukin-10 production. Located in extracellular region. Implicated in several diseases, including allergic disease (multiple); autoimmune disease (multiple); diffuse scleroderma; lung disease (multiple); and nose disease (multiple). Biomarker of several diseases, including Plasmodium falciparum malaria; autoimmune disease (multiple); cystic fibrosis; lung disease (multiple); and scleroderma (multiple).

操作步骤


下载本产品说明书

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

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引用文献

文章目录[隐藏]


  1. Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 

  2. Elevated serum IL-13 level is associated with increased Treg cells in tumor microenvironment and disease progression of diffuse large B-cell lymphoma 

  3. Mesenchymal stromal cells pretreated with proinflammatory cytokines enhance skin wound healing via IL-6-dependent M2 polarization 

  4. Type 2 innate lymphoid cells participate in IL‑33‑stimulated Th2‑associated immune response in chronic obstructive pulmonary disease 

  5. Downregulation of miRNA-451a Promotes the Differentiation of CD4+ T Cells towards Th2 Cells by Upregulating ETS1 in Childhood Asthma 

  6. Sputum basophils from allergic asthmatic patients do not express IL-7Rα that is essential for TSLP signalling 

  7. Regulation of CBL and ESR1 expression by microRNA-22‑3p, 513a-5p and 625-5p may impact the pathogenesis of dust mite-induced pediatric asthma 

  8. ILC2 Cells Promote Th2 Cell Differentiation in AECOPD Through Activated Notch-GATA3 Signaling Pathway 

  9. ILC2s Induce Adaptive Th2-Type Immunity in Acute Exacerbation of Chronic Obstructive Pulmonary Disease 

  10. Interleukin 3-induced GITR promotes the activation of human basophils 

  11. Induction of the apoptosis, degranulation and IL-13 production of human basophils by butyrate and propionate via suppression of histone deacetylation 

  12. Effects of Sarcoptes scabiei Translationally Controlled Tumor Protein (TCTP) on Histamine Release and Degranulation of KU812 Cells 

  13. IgD-activated mast cells induce IgE synthesis in B cells in nasal polyps 

  14. c-CBL/LCK/c-JUN/ETS1/CD28 axis restrains childhood asthma by suppressing Th2 differentiation 

  15. Targeting mitochondrial dysfunction in atopic dermatitis with trilinolein: a triacylglycerol from the medicinal plant Cannabis fructus 

  16. CLDN1 silencing suppresses the proliferation and migration of airway smooth muscle cells by modulating MMP14 

  17. The enrichment of Arg1+ILC2s and ILCregs facilitates the progression of endometriosis: A preliminary study 

  18. Silibinin attenuated pseudo-allergic reactions and mast cell degranulation via PLCγ and PI3K/Akt signaling pathway 

  19. 腹腔上清液

    Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 

  20. Activation of Tgf-Β1/Α-Sma/Col I Profibrotic Pathway in Fibroblasts by Galectin-3 Contributes to Atrial Fibrosis in Experimental Models and Patients 

  21. Induction of the Apoptosis, Degranulation and Il-13 Production of Human Basophils by Butyrate and Propionate Via Suppression of Histone Deacetylation 

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