EK112

Human IL-12p70 ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：IL12, IL-12, CLMF
  • 种属：人 (Human)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：血清血浆：10 μL；细胞培养上清：100 μL
  • 灵敏度：0.32 pg/mL
  • 检测范围：1.56 pg/mL - 100 pg/mL
  • 回收率：84% - 119%

在售SKU:70-EK112-48, 70-EK112-96, 人, 白介素12, 白介12, IL12, interleukin 12

描述

商品名

Human IL-12p70 ELISA Kit (人白介素12p70 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：5.5% - 7.5%；板间变异系数：3.2% - 7.5%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

血清血浆：10 μL；细胞培养上清：100 μL
灵敏度

0.32 pg/mL
检测范围

1.56 pg/mL - 100 pg/mL
回收率

84% - 119%
平均回收率

1.03
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IL-12p70单克隆抗体的96孔聚苯乙烯酶标板
  • 人IL-12p70冻干标准品
  • IL-12p70检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物（TMB），避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应，在450nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IL-12p70 ELISA Kit (人白介素12p70 ELISA试剂盒) - 标准曲线
标准曲线
IL12A 分子靶点信息概述IL12B 分子靶点信息概述

IL12A 分子靶点信息概述

  • 分子名：IL12A, interleukin 12A
  • 基因家族：Interleukins
  • 别名：CLMF; IL-12A; p35; NFSK
  • 曾用名：NKSF1
  • 全称：nterleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); natural killer cell stimulatory factor 1, 35 kD subunit; cytotoxic lymphocyte maturation factor 1, p35; interleukin 12, p35; IL-12, subunit p35; NF cell stimulatory factor chain 1; interleukin-12 alpha chain; IL35 subunit

IL12B 分子靶点信息概述

  • 分子名：IL12B, interleukin 12B
  • 基因家族：Immunoglobulin like domain containing; Interleukins
  • 别名：CLMF; IL-12B; NKSF; CLMF2
  • 曾用名：NKSF2
  • 全称：natural killer cell stimulatory factor-2; cytotoxic lymphocyte maturation factor 2, p40; interleukin 12, p40; natural killer cell stimulatory factor, 40 kD subunit; interleukin-12 beta chain; IL12, subunit p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)

IL12B 分子靶点综述

白细胞介素12 (IL-12)由树突状细胞、巨噬细胞、中性粒细胞和人B淋巴母细胞株 (NC-37)在响应抗原刺激时自然产生。IL-12由4个а螺旋组成，是一个由2种独立基因--- IL-12A (p35)和IL-12B (p40)编码形成的异源二聚体细胞因子。蛋白合成之后可形成有生物活性的异源二聚体(p70)和同源二聚体p40。IL-12参与初始T细胞向Th1细胞分化的过程，作为T细胞刺激因子，它可刺激T细胞的生长及其功能发挥。IL-12具有抗血管生成活性，对自然杀伤细胞和T淋巴细胞活性有重要作用。同样地，IL-12与自身免疫相关。

IL12, interleukin 12 分子靶点种属信息

人 Human IL1A人 Human IL1B

人 Human IL12A 分子靶点信息

  • 分子名：IL12A, interleukin 12A
  • 别称：
    • CLMF
    • CLMF p35
    • cytotoxic lymphocyte maturation factor 1, p35
    • cytotoxic lymphocyte maturation factor 35 kDa subunit
    • IL-12 subunit p35
    • IL-12, subunit p35
    • IL-12A
    • IL35 subunit
    • interleukin 12, p35
    • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
    • interleukin-12 alpha chain
    • interleukin-12 subunit alpha
    • natural killer cell stimulatory factor 1, 35 kD subunit
    • NF cell stimulatory factor chain 1
    • NFSK
    • NK cell stimulatory factor chain 1
    • NKSF1
    • P35
  • 基因序列：NCBI_Gene: 3592
  • 蛋白序列：UniProtKB: P29459

人 Human IL12A靶点分子功能（预测）

Enables interleukin-12 beta subunit binding activity; interleukin-27 binding activity; and protein heterodimerization activity. Contributes to cytokine activity and growth factor activity. Involved in several processes, including positive regulation of leukocyte mediated cytotoxicity; positive regulation of lymphocyte proliferation; and response to bacterium. Acts upstream of or within negative regulation of protein secretion. Located in extracellular space. Part of interleukin-12 complex. Implicated in hepatitis C; hepatocellular carcinoma; and primary biliary cholangitis. Biomarker of hepatitis C.

人 Human IL12B 分子靶点信息

  • 分子名：IL12B, interleukin 12B
  • 别称：
    • CLMF
    • CLMF p40
    • CLMF2
    • cytotoxic lymphocyte maturation factor 40 kDa subunit
    • IL-12 subunit p40
    • IL-12B
    • IL12, subunit p40
    • IMD28
    • IMD29
    • interleukin 12, p40
    • interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2
    • interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
    • interleukin-12 beta chain
    • interleukin-12 subunit beta
    • natural killer cell stimulatory factor, 40 kD subunit
    • NK cell stimulatory factor chain 2
    • NKSF
    • NKSF2
  • 基因序列：NCBI_Gene: 3593
  • 蛋白序列：UniProtKB: P29460

人 Human IL12B靶点分子功能（预测）

Enables identical protein binding activity; interleukin-12 alpha subunit binding activity; and protein heterodimerization activity. Contributes to cytokine activity; growth factor activity; and interleukin-23 receptor binding activity. Involved in several processes, including positive regulation of lymphocyte proliferation; positive regulation of peptidyl-tyrosine phosphorylation; and regulation of cytokine production. Acts upstream of or within negative regulation of protein secretion. Part of interleukin-12 complex and interleukin-23 complex. Implicated in asthma; chronic obstructive pulmonary disease; immunodeficiency 29; and salmonellosis. Biomarker of anogenital venereal wart.

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]

  1. Tumor associated neutrophils promote the metastasis of pancreatic ductal adenocarcinoma 

  2. Involvement of impaired CD8+ mucosal-associated invariant T cells and myeloid-derived suppressor cells in polycystic ovary syndrome 

  3. Macrophage polarization toward M1 phenotype through NF-κB signaling in patients with Behçet’s disease 

  4. Dynamic changes of innate lymphoid cells in acute ST-segment elevation myocardial infarction and its association with clinical outcomes 

  5. Long Noncoding RNA and Circular RNA Expression Profiles of Monocyte-Derived Dendritic Cells in Autoimmune Hepatitis 

  6. MicroRNA 27b-3p Modulates SYK in Pediatric Asthma Induced by Dust Mites 

  7. CD4+/CD8+ mucosa-associated invariant T cells foster the development of endometriosis: a pilot study 

  8. A novel long-term intravenous combined with local treatment with human amnion-derived mesenchymal stem cells for a multidisciplinary rescued uremic calciphylaxis patient and the underlying mechanism 

  9. High-Dose Aluminum Exposure Further Alerts Immune Phenotype in Aplastic Anemia Patients 

  10. Cell-free miR-17-5p as a diagnostic biomarker for gastric cancer inhibits dendritic cell maturation 

  11. Target-Dependent Expression of IL12 by synNotch Receptor-Engineered NK92 Cells Increases the Antitumor Activities of CAR-T Cells 

  12. Circulating CD14+CD163+CD206+ M2 Monocytes Are Increased in Patients with Early Stage of Idiopathic Membranous Nephropathy 

  13. Myeloid-derived suppressor cell cytokine secretion as prognostic factor in myelodysplastic syndromes 

  14. Comparison of morphology, phenotypes and function between cultured human IL‑4‑DC and IFN‑DC 

  15. Participation of Increased Circulating MAIT Cells in Lung Cancer: a Pilot Study 

  16. The clinical study on treatment of CD19-directed chimeric antigen receptor-modified T cells in a case of refractory Richter syndrome 

  17. Follistatin-Like Protein-1 Upregulates Dendritic Cell-Based Immunity in Patients with Nasopharyngeal Carcinoma 

  18. Armored Inducible Expression of IL-12 Enhances Antitumor Activity of Glypican-3–Targeted Chimeric Antigen Receptor–Engineered T Cells in Hepatocellular Carcinoma 

  19. Efficacy and safety of low-dose interleukin-2 in combination with methotrexate in patients with active rheumatoid arthritis: a randomized, double-blind, placebo-controlled phase 2 trial 

  20. Infant immune response to hepatitis B vaccine after fetal exposure to telbivudine 

  21. Lyophilized lymph nodes for improved delivery of chimeric antigen receptor T cells 

  22. P-Hydroxylcinnamaldehyde induces tumor-associated macrophage polarization toward the M1 type by regulating the proteome and inhibits ESCC in vivo and in vitro 

  23. 腹水 血浆

    CD4+/CD8+ mucosa-associated invariant T cells foster the development of endometriosis: a pilot study 

  24. 腹腔上清液

    Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 

  25. 血清

    Participation of Increased Circulating MAIT Cells in Lung Cancer: a Pilot Study 

  26. 血浆

    Involvement of Impaired Cd8+ Mucosal-Associated Invariant T Cells and Myeloid-Derived Suppressor Cells in Polycystic Ovary Syndrome 

  27. 血清

    Long Noncoding Rna and Circular Rna Expression Profiles of Monocyte-Derived Dendritic Cells in Autoimmune Hepatitis 

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