EK171
Human IgG ELISA Kit检测试剂盒（酶联免疫吸附法）
¥1,600.00 – ¥2,650.00
因产品会迭代升级，具体实验步骤请按纸质版说明书操作
- 分子靶点：IgG
- 种属：人 (Human)
- 样本类型：血清，血浆，细胞培养上清及其他生物学样本
- 检测样本体积：100 μL
- 灵敏度：0.18 ng/mL
- 检测范围：3.13 ng/mL - 200 ng/mL
- 回收率：93% - 100%
在售SKU:70-EK171-48, 70-EK171-96, 免疫球蛋白, 球蛋白, 免疫球蛋白G, 球蛋白G, IgG, Immunoglobulins, G
描述
| 商品名 |
Human IgG ELISA Kit (人免疫球蛋白G ELISA试剂盒) |
|---|---|
| 检测方法 |
双抗夹心法 |
| 精密度 |
板内变异系数：5.5% - 7.6%；板间变异系数：5.6% - 8.1% |
| 样本类型 |
血清，血浆，细胞培养上清及其他生物学样本 |
| 检测样本体积 |
100 μL |
| 灵敏度 |
0.18 ng/mL |
| 检测范围 |
3.13 ng/mL - 200 ng/mL |
| 回收率 |
93% - 100% |
| 平均回收率 |
1.04 |
| 板式 |
96孔板，可拆 |
| 保存 |
试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。 |
| 运输条件 |
4℃蓝冰运输 |
| 组分 |
|
检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IgG抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本进行孵育。经过孵育，样本中存在的IgG与固相抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的检测抗体孵育。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IgG的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
ELISA试剂盒操作常见问题查看更多ELISA试剂盒操作步骤技术文章
查看更多ELISA试剂盒操作步骤技术文章
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 一文掌握ELISA实验显色判断、数据分析及标曲拟合
- 血清OR血浆，哪个是ELISA的菜
- ELISA通关必备丨数据篇丨标准曲线不佳
- ELISA通关必备丨操作篇丨常见问题及解决方案
- ELISA通关必备丨操作篇丨溶解与稀释标准品
- ELISA通关必备丨样本篇丨不常见样本
- ELISA通关必备丨样本篇丨常见样本丨细胞
- ELISA通关必备丨如何选择试剂盒
- ELISA通关必备丨基础知识
- 真？假？ELISA试剂盒选择要小心
- ELISA常见类型一 | 双抗夹心法，你要的都在这里！
- ELISA常见类型二 | 竞争法，五分钟搞定！
- 叮！联科向您投递了个ELISA实验操作干货包，请查收~
- 【视频】ELISA实验操作步骤演示视频教程
- ELISA 组织样本的处理—大鼠组织
- 【视频】ELISA实验原理与常见问题分析
- 查看更多ELISA操作相关问题
ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- Reduced Energy Metabolism Impairs T Cell-Dependent B Cell Responses in Patients With Advanced HBV-Related Cirrhosis
- A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide.
- Single-cell and spatial transcriptome analyses reveal tertiary lymphoid structures linked to tumour progression and immunotherapy response in nasopharyngeal carcinoma
- Characterization of the MicroRNA profile in rheumatoid arthritis plasma exosomes and their roles in B-cell responses
- Autoantibody repertoire profiling in tissue and blood identifies colorectal cancer-specific biomarkers
- Development of a mammalian cell-based ZZ display system for IgG quantification
- Flower-like WSe2 used as bio-matrix in ultrasensitive label-free electrochemical immunosensor for human immunoglobulin G determination
- Development of a mammalian cell-based ZZ display system for IgG quantification
- Reduced Energy Metabolism Impairs T Cell-Dependent B Cell Responses in Patients With Advanced HBV-Related Cirrhosis
- A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide
Reduced Energy Metabolism Impairs T Cell-Dependent B Cell Responses in Patients With Advanced HBV-Related Cirrhosis
A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide.
Single-cell and spatial transcriptome analyses reveal tertiary lymphoid structures linked to tumour progression and immunotherapy response in nasopharyngeal carcinoma
Characterization of the MicroRNA profile in rheumatoid arthritis plasma exosomes and their roles in B-cell responses
Autoantibody repertoire profiling in tissue and blood identifies colorectal cancer-specific biomarkers
Development of a mammalian cell-based ZZ display system for IgG quantification
Flower-like WSe2 used as bio-matrix in ultrasensitive label-free electrochemical immunosensor for human immunoglobulin G determination
Development of a mammalian cell-based ZZ display system for IgG quantification
Reduced Energy Metabolism Impairs T Cell-Dependent B Cell Responses in Patients With Advanced HBV-Related Cirrhosis
A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide
成为第一个“Human IgG ELISA Kit检测试剂盒（酶联免疫吸附法）” 的评价者
引用文献统计
该产品被引用的文献总数为：10
- Reduced Energy Metabolism Impairs T Cell-Dependent B Cell Responses in Patients With Advanced HBV-Related Cirrhosis
影响因子：7.561刊物：Frontiers in Immunology发表日期：2021/6/23 - A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide.
影响因子：8.786刊物：Frontiers in Immunology发表日期：2022/10/19 - Single-cell and spatial transcriptome analyses reveal tertiary lymphoid structures linked to tumour progression and immunotherapy response in nasopharyngeal carcinoma
影响因子：14.7刊物：Nature Communications发表日期：2024-09-04 - Characterization of the MicroRNA profile in rheumatoid arthritis plasma exosomes and their roles in B-cell responses
影响因子：2.2刊物：Clinics发表日期：2024-07-07 - Autoantibody repertoire profiling in tissue and blood identifies colorectal cancer-specific biomarkers
影响因子：5.7刊物：CANCER SCIENCE发表日期：2023-11-20 - Development of a mammalian cell-based ZZ display system for IgG quantification
影响因子：3.5刊物：BMC BIOTECHNOLOGY发表日期：2023-07-28 - Flower-like WSe2 used as bio-matrix in ultrasensitive label-free electrochemical immunosensor for human immunoglobulin G determination
影响因子：1.6刊物：ANALYTICAL SCIENCES发表日期：2023-05-25 - Development of a mammalian cell-based ZZ display system for IgG quantification
影响因子：3.5刊物：BMC Biotechnol. 2023 Jul 28;23(1):24. - Reduced Energy Metabolism Impairs T Cell-Dependent B Cell Responses in Patients With Advanced HBV-Related Cirrhosis
影响因子：7.561刊物：Front Immunol. 2021 Jun 23;12:660312. - A metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide
影响因子：8.786刊物：Front Immunol. 2022 Oct 19;13:1018567.
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