EK1131

Human IGF-1 ELISA Kit检测试剂盒(酶联免疫吸附法)

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¥1,600.00¥2,650.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:IGF1, IGFI
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:100 μL
  • 灵敏度:15.35 pg/mL
  • 检测范围:62.5 pg/mL - 4000 pg/mL
  • 回收率:95% - 108%

在售SKU:70-EK1131-48, 70-EK1131-96


描述

商品名

Human IGF-1 ELISA Kit (人胰岛素样生长因子1 ELISA试剂盒)

检测方法

双抗夹心法

精密度

板内变异系数:2.8% - 4.5%;板间变异系数:4.6% - 7.4%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

100 μL

灵敏度

15.35 pg/mL

检测范围

62.5 pg/mL - 4000 pg/mL

回收率

95% - 108%

平均回收率

1.02

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗IGF-1单克隆抗体的96孔聚苯乙烯酶标板
  • 人IGF-1冻干标准品
  • IGF-1检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IGF-1抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IGF-1与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IGF-1的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IGF-1 ELISA Kit (人胰岛素样生长因子1 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 IGF1, IGFI 靶点信息

IGF1 分子靶点信息概述

  • 分子名:IGF1, insulin like growth factor 1
  • 基因家族:Neuropeptides
  • 别名:IGF1A; IGFI; IGF-I; IGF
  • 全称:somatomedin C; insulin-like growth factor 1 (somatomedin C)

IGF1 分子靶点综述

胰岛素样生长因子1(IGF-1),也称为生长调节素C,是一个在分子结构上类似于胰岛素的激素。IGF-1主要由肝脏产生,作为内分泌激素。同样地,靶组织亦能旁分泌/自分泌产生IGF-1。IGF-1是AKT信号转导途径中的一种有效天然激活剂、细胞生长和增殖的刺激子、细胞程序性死亡的有效抑制子。IGF-1最少可结合两种细胞表面受体:IGF-1受体(IGF1R)和胰岛素受体。IGF-1的产生受生长激素刺激,受营养不良、生长激素不敏感、缺少生长激素受体或者生长激素受体(SHP2和STAT5B)下游信号通路发生故障等方面所抑制。IGF-1在儿童生长过程中起重要作用,继而在成人合成代谢中有影响,且与老年化、神经病变、癌症以及中风相关。

人 Human IGF1 分子靶点信息

  • 分子名:IGF1, insulin like growth factor 1
  • 别称:
    • IGF
    • IGF-I
    • IGF-IA
    • IGF-IB
    • IGF1A
    • IGFI
    • insulin-like growth factor 1
    • insulin-like growth factor 1 (somatomedin C)
    • insulin-like growth factor I
    • insulin-like growth factor IA
    • insulin-like growth factor IB
    • mechano growth factor
    • MGF
    • somatomedin-C
  • 基因序列:NCBI_Gene: 3479
  • 蛋白序列:UniProtKB: P05019

人 Human IGF1靶点分子功能(预测)

Enables signaling receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Acts upstream of or within cell activation. Located in extracellular space. Part of alphav-beta3 integrin-IGF-1-IGF1R complex and insulin-like growth factor ternary complex. Implicated in several diseases, including acne; brain ischemia; diabetes mellitus (multiple); myopia; and neurodegenerative disease (multiple). Biomarker of several diseases, including Henoch-Schoenlein purpura; artery disease (multiple); autoimmune disease (multiple); bone disease (multiple); and eye disease (multiple).

操作步骤

ELISA操作常见问题

查看更多ELISA操作相关问题

引用文献


  1. Growth factors-based platelet lysate rejuvenates skin against ageing through NF-κB signalling pathway: In vitro and in vivo mechanistic and clinical studies 

  2. Advanced-platelet-rich fibrin extract promotes adipogenic and osteogenic differentiation of human adipose-derived stem cells in a dose-dependent manner in vitro 

  3. Growth factors-based beneficial effects of platelet lysate on umbilical cord-derived stem cells and their synergistic use in osteoarthritis treatment 

  4. Multiple Intravenous Injections of Valproic Acid-Induced Mesenchymal Stem Cell from Human-Induced Pluripotent Stem Cells Improved Cardiac Function in an Acute Myocardial Infarction Rat Model 

  5. Microtia patients: Auricular chondrocyte ECM is promoted by CGF through IGF-1 activation of the IGF-1R/PI3K/AKT pathway 

  6. SHP2-Triggered Endothelial Cell Activation Fuels Estradiol-Independent Endometrial Sterile Inflammation 

  7. Providing biomimetic microenvironment for pulp regeneration via hydrogel-mediated sustained delivery of tissue-specific developmental signals 

  8. Mesenchymal stem cells derived from CHIR99021 and TGF?β induction remained on the colicomentum and improved cardiac function of a rat model of acute myocardium infarction 

  9. UCHL1 aggravates skin fibrosis through an IGF-1-induced Akt/mTOR/HIF-1α pathway in keloid 

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