Human IGF-1 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:IGF1, IGFI
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:100 μL
- 灵敏度:15.35 pg/mL
- 检测范围:62.5 pg/mL - 4000 pg/mL
- 回收率:95% - 108%
在售SKU:70-EK1131-48, 70-EK1131-96
描述
商品名 |
Human IGF-1 ELISA Kit (人胰岛素样生长因子1 ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:2.8% - 4.5%;板间变异系数:4.6% - 7.4% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
100 μL |
灵敏度 |
15.35 pg/mL |
检测范围 |
62.5 pg/mL - 4000 pg/mL |
回收率 |
95% - 108% |
平均回收率 |
1.02 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IGF-1抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的IGF-1与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IGF-1的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
IGF1 分子靶点信息概述
- 分子名:IGF1, insulin like growth factor 1
- 基因家族:Neuropeptides
- 别名:IGF1A; IGFI; IGF-I; IGF
- 全称:somatomedin C; insulin-like growth factor 1 (somatomedin C)
IGF1 分子靶点综述
胰岛素样生长因子1(IGF-1),也称为生长调节素C,是一个在分子结构上类似于胰岛素的激素。IGF-1主要由肝脏产生,作为内分泌激素。同样地,靶组织亦能旁分泌/自分泌产生IGF-1。IGF-1是AKT信号转导途径中的一种有效天然激活剂、细胞生长和增殖的刺激子、细胞程序性死亡的有效抑制子。IGF-1最少可结合两种细胞表面受体:IGF-1受体(IGF1R)和胰岛素受体。IGF-1的产生受生长激素刺激,受营养不良、生长激素不敏感、缺少生长激素受体或者生长激素受体(SHP2和STAT5B)下游信号通路发生故障等方面所抑制。IGF-1在儿童生长过程中起重要作用,继而在成人合成代谢中有影响,且与老年化、神经病变、癌症以及中风相关。
人 Human IGF1 分子靶点信息
- 分子名:IGF1, insulin like growth factor 1
- 别称:
- IGF
- IGF-I
- IGF-IA
- IGF-IB
- IGF1A
- IGFI
- insulin-like growth factor 1
- insulin-like growth factor 1 (somatomedin C)
- insulin-like growth factor I
- insulin-like growth factor IA
- insulin-like growth factor IB
- mechano growth factor
- MGF
- somatomedin-C
- 基因序列:NCBI_Gene: 3479
- 蛋白序列:UniProtKB: P05019
人 Human IGF1靶点分子功能(预测)
Enables signaling receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of macromolecule metabolic process; and positive regulation of signal transduction. Acts upstream of or within cell activation. Located in extracellular space. Part of alphav-beta3 integrin-IGF-1-IGF1R complex and insulin-like growth factor ternary complex. Implicated in several diseases, including acne; brain ischemia; diabetes mellitus (multiple); myopia; and neurodegenerative disease (multiple). Biomarker of several diseases, including Henoch-Schoenlein purpura; artery disease (multiple); autoimmune disease (multiple); bone disease (multiple); and eye disease (multiple).
操作步骤
文章目录[隐藏]
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引用文献
文章目录[隐藏]
- Growth factors-based platelet lysate rejuvenates skin against ageing through NF-κB signalling pathway: In vitro and in vivo mechanistic and clinical studies 
- Advanced-platelet-rich fibrin extract promotes adipogenic and osteogenic differentiation of human adipose-derived stem cells in a dose-dependent manner in vitro 
- Growth factors-based beneficial effects of platelet lysate on umbilical cord-derived stem cells and their synergistic use in osteoarthritis treatment 
- Multiple Intravenous Injections of Valproic Acid-Induced Mesenchymal Stem Cell from Human-Induced Pluripotent Stem Cells Improved Cardiac Function in an Acute Myocardial Infarction Rat Model 
- Microtia patients: Auricular chondrocyte ECM is promoted by CGF through IGF-1 activation of the IGF-1R/PI3K/AKT pathway 
- SHP2-Triggered Endothelial Cell Activation Fuels Estradiol-Independent Endometrial Sterile Inflammation 
- Providing biomimetic microenvironment for pulp regeneration via hydrogel-mediated sustained delivery of tissue-specific developmental signals 
- Mesenchymal stem cells derived from CHIR99021 and TGF?β induction remained on the colicomentum and improved cardiac function of a rat model of acute myocardium infarction 
- UCHL1 aggravates skin fibrosis through an IGF-1-induced Akt/mTOR/HIF-1α pathway in keloid 
Growth factors-based platelet lysate rejuvenates skin against ageing through NF-κB signalling pathway: In vitro and in vivo mechanistic and clinical studies 
Advanced-platelet-rich fibrin extract promotes adipogenic and osteogenic differentiation of human adipose-derived stem cells in a dose-dependent manner in vitro 
Growth factors-based beneficial effects of platelet lysate on umbilical cord-derived stem cells and their synergistic use in osteoarthritis treatment 
Multiple Intravenous Injections of Valproic Acid-Induced Mesenchymal Stem Cell from Human-Induced Pluripotent Stem Cells Improved Cardiac Function in an Acute Myocardial Infarction Rat Model 
Microtia patients: Auricular chondrocyte ECM is promoted by CGF through IGF-1 activation of the IGF-1R/PI3K/AKT pathway 
SHP2-Triggered Endothelial Cell Activation Fuels Estradiol-Independent Endometrial Sterile Inflammation 
Providing biomimetic microenvironment for pulp regeneration via hydrogel-mediated sustained delivery of tissue-specific developmental signals 
Mesenchymal stem cells derived from CHIR99021 and TGF?β induction remained on the colicomentum and improved cardiac function of a rat model of acute myocardium infarction 
UCHL1 aggravates skin fibrosis through an IGF-1-induced Akt/mTOR/HIF-1α pathway in keloid 
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