EK1236

Human IFN-β ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥1,600.00 – ¥2,650.00

因产品会迭代升级，具体实验步骤请按纸质版说明书操作

  • 分子靶点：IFNB1, IFN-β, IFNB
  • 种属：人 (Human)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：50 μL
  • 灵敏度：3.19 pg/mL
  • 检测范围：15.63 pg/mL - 1000 pg/mL
  • 回收率：79% - 95%

在售SKU:70-EK1236-48, 70-EK1236-96

描述

商品名

Human IFN-β ELISA Kit (人 IFN-β ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：4.5% - 5.0%；板间变异系数：3.6% - 7.9%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

50 μL
灵敏度

3.19 pg/mL
检测范围

15.63 pg/mL - 1000 pg/mL
回收率

79% - 95%
平均回收率

0.88
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗IFN-β单克隆抗体的96孔聚苯乙烯酶标板
  • 人IFN-β冻干标准品
  • IFN-β检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IFN-β抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IFN-β与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IFN-β的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human IFN-β ELISA Kit (人 IFN-β ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 IFNB1, IFN-β, IFNB 靶点信息

IFNB1 分子靶点信息概述

  • 分子名：IFNB1, interferon beta 1
  • 基因家族：Interferons
  • 别名：IFB; IFF
  • 曾用名：IFNB
  • 全称：interferon, beta 1, fibroblast

IFNB1 分子靶点综述

干扰素(IFNs)是宿主细胞在响应病原体(如病毒、细菌、寄生虫或者肿瘤细胞)时合成和释放的一组信号蛋白。在通常情况下，病毒感染的细胞会释放干扰素，使周围的细胞提高其抗病毒防御能力。基于受体类型，人类干扰素可分为3大类型：I型干扰素，包括IFN-α、IFN-β、IFN-ε、IFN-κ和IFN-ω，II型干扰素(在人类中称为IFN-γ)和III型干扰素。IFN-β通过信号通路STAT1和STAT2上调和下调多种基因，其中，大部分基因参与抗病毒免疫反应。IFN-β在诱导广泛的非特异性抗病毒感染中起重要作用，它还影响细胞增殖并调节免疫应答。

人 Human IFNB1 分子靶点信息

  • 分子名：IFNB1, interferon beta 1
  • 别称：
    • fibroblast interferon
    • IFB
    • IFF
    • IFN-beta
    • IFNB
    • interferon beta
    • interferon-beta
    • interferon, beta 1
    • interferon, beta 1, fibroblast
    • MGC96956
  • 基因序列：NCBI_Gene: 3456
  • 蛋白序列：UniProtKB: P01574

人 Human IFNB1靶点分子功能（预测）

Enables chloramphenicol O-acetyltransferase activity and cytokine activity. Involved in several processes, including cellular response to cytokine stimulus; negative regulation of T-helper 2 cell cytokine production; and positive regulation of nitrogen compound metabolic process. Acts upstream of or within response to exogenous dsRNA. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Implicated in COVID-19.

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. Cathelicidin antimicrobial peptides suppress EV71 infection via regulating antiviral response and inhibiting viral binding 

  2. Lonidamine potentiates the oncolytic efficiency of M1 virus independent of hexokinase 2 but via inhibition of antiviral immunity 

  3. Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages 

  4. Oroxylin A regulates cGAS DNA hypermethylation induced by methionine metabolism to promote HSC senescence 

  5. Allosteric inhibition reveals SHP2-mediated tumor immunosuppression in colon cancer by single-cell transcriptomics 

  6. LncNSPL facilitates influenza A viral immune escape by restricting TRIM25-mediated K63-linked RIG-I ubiquitination 

  7. A C-terminal glutamine recognition mechanism revealed by E3 ligase TRIM7 structures 

  8. Structural insights into a shared mechanism of human STING activation by a potent agonist and an autoimmune disease-associated mutation 

  9. Single-cell landscape of immunological responses in patients with COVID-19 

  10. Anemoside B4 inhibits enterovirus 71 propagation in mice through upregulating 14-3-3 expression and type I interferon responses 

  11. Lipopolysaccharide enhances DNA‑induced IFN‑β expression and autophagy by upregulating cGAS expression in A549 cells 

  12. RIPK3-Dependent Necroptosis Is Induced and Restricts Viral Replication in Human Astrocytes Infected With Zika Virus 

  13. Allicin Inhibits Proliferation by Decreasing IL-6 and IFN-β in HCMV-Infected Glioma Cells 

  14. STING agonist cGAMP enhances anti-tumor activity of CAR-NK cells against pancreatic cancer 

  15. Targeted Delivery of STING Agonist via Albumin Nanoreactor Boosts Immunotherapeutic Efficacy against Aggressive Cancers 

  16. Exploring expression levels of the cGAS-STING pathway genes in peripheral blood mononuclear cells of spinal tuberculosis patients 

  17. STING-mediated IL-6 Inhibits OATP1B1 Expression via the TCF4 Signaling Pathway in Cholestasis 

  18. TRIM38 Induced in Respiratory Syncytial Virus-infected Cells Downregulates Type I Interferon Expression by Competing with TRIM25 to Bind RIG-I 

  19. Luteolin-7-O-glucoside promotes macrophage release of IFN-β by maintaining mitochondrial function and corrects the disorder of glucose metabolism during RSV infection 

  20. RNA methyltransferase FTSJ3 regulates the type I interferon pathway to promote hepatocellular carcinoma immune evasion 

  21. Human endogenous retroviruses as epigenetic therapeutic targets in TP53-mutated diffuse large B-cell lymphoma 

  22. The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication by Suppressing Innate Immunity via Interaction with RNA Helicase DDX1 

  23. IFN-beta and EIF2AK2 are potential biomarkers for interstitial lung disease in anti-MDA5 positive dermatomyositis 

  24. 细胞培养上清

    Anemoside B4 inhibits enterovirus 71 propagation in mice through upregulating 14-3-3 expression and type I interferon responses 

  25. Lipopolysaccharide Enhances DNA-Induced Ifn-Β Expression and Autophagy by Upregulating Cgas Expression in A549 Cells 

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