Human IFN-α High Sensitivity ELISA Kit检测试剂盒(酶联免疫吸附法)
¥2,000.00 – ¥3,400.00
- 分子靶点:IFNA1, IFN-α
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:20 μL
- 灵敏度:0.23 pg/mL
- 检测范围:7.81 pg/mL - 500 pg/mL
- 回收率:95% - 119%
在售SKU:70-EK199HS-48, 70-EK199HS-96
描述
商品名 |
Human IFN-α High Sensitivity ELISA Kit (人干扰素 (IFNs) 高敏 ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:4.1% - 5.0%;板间变异系数:3.9% - 4.9% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
20 μL |
灵敏度 |
0.23 pg/mL |
检测范围 |
7.81 pg/mL - 500 pg/mL |
回收率 |
95% - 119% |
平均回收率 |
1.05 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人IFN-α抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的IFN-α与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入信号增强剂孵育,洗涤去除未结合的物质后,再次加入Streptavidin-HRP。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中IFN-α的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
IFNA1 分子靶点信息概述
- 分子名:IFNA1, interferon alpha 1
- 基因家族:Interferons
- 别名:IFNA@; IFL; IFN; IFN-ALPHA; IFNA13; IFN-alphaD
- 全称:IFN-alpha 1b; interferon alpha 1b
IFNA1 分子靶点综述
干扰素(IFNs)是宿主细胞在响应病原体(如病毒、细菌、寄生虫或者肿瘤细胞)时合成和释放的一组信号蛋白。在通常情况下,病毒感染的细胞会释放干扰素,使周围的细胞提高其抗病毒防御能力。基于受体类型,人类干扰素可分为3大类型:I型干扰素,包括IFN-α、IFN-β、IFN-ε、IFN-κ和IFN-ω,II型干扰素(在人类中称为IFN-γ)和III型干扰素。IFN-α蛋白由白细胞产生,主要参与响应病毒感染的先天性免疫。研究显示加强肿瘤浸润巨噬细胞中IFN-α的表达可引起更有效的树突状细胞活化和免疫效应细胞毒作用。IFN-α作为致热因子,可通过改变下丘脑中热敏神经元的活性来导致发热。运用类似机理,IFN-α可用来减轻疼痛,与μ-阿片受体相互作用来作为止痛剂。
人 Human IFNA1 分子靶点信息
- 分子名:IFNA1, interferon alpha 1
- 别称:
- IFL
- IFN
- IFN-ALPHA
- IFN-alpha 1b
- IFN-alpha-1/13
- IFN-alphaD
- IFNA@
- IFNA13
- interferon alpha 1b
- interferon alpha-1/13
- interferon alpha-D
- interferon-alpha1
- interferon, alpha 1
- leIF D
- MGC138207
- MGC138505
- MGC138507
- 基因序列:NCBI_Gene: 3439
- 蛋白序列:UniProtKB: P01562
人 Human IFNA1靶点分子功能(预测)
Predicted to enable cytokine activity and type I interferon receptor binding activity. Predicted to be involved in several processes, including B cell activation; lymphocyte activation involved in immune response; and positive regulation of peptidyl-serine phosphorylation of STAT protein. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Implicated in Crimean-Congo hemorrhagic fever and hepatocellular carcinoma. Biomarker of melioidosis.
操作步骤
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引用文献
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- Lonidamine potentiates the oncolytic efficiency of M1 virus independent of hexokinase 2 but via inhibition of antiviral immunity 
- Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 
- Expression profile of PU.1 in CD4+T cells from patients with systemic lupus erythematosus 
- Infant immune response to hepatitis B vaccine after fetal exposure to telbivudine 
- TRIM38 Induced in Respiratory Syncytial Virus-infected Cells Downregulates Type I Interferon Expression by Competing with TRIM25 to Bind RIG-I 
- The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication by Suppressing Innate Immunity via Interaction with RNA Helicase DDX1 
Lonidamine potentiates the oncolytic efficiency of M1 virus independent of hexokinase 2 but via inhibition of antiviral immunity 
Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 
Expression profile of PU.1 in CD4+T cells from patients with systemic lupus erythematosus 
Infant immune response to hepatitis B vaccine after fetal exposure to telbivudine 
TRIM38 Induced in Respiratory Syncytial Virus-infected Cells Downregulates Type I Interferon Expression by Competing with TRIM25 to Bind RIG-I 
The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication by Suppressing Innate Immunity via Interaction with RNA Helicase DDX1 
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