Human G-CSF ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:CSF3, G-CSF
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:80 μL 1×检测缓冲液和20 μL样本
- 灵敏度:6.04 pg/mL
- 检测范围:31.25 pg/mL - 2000 pg/mL
- 回收率:88% - 116%
在售SKU:70-EK169-48, 70-EK169-96
描述
商品名 |
Human G-CSF ELISA Kit (人粒细胞集落刺激因子ELISA 试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:7.4% - 7.8%;板间变异系数:8.3% - 12.6% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
80 μL 1×检测缓冲液和20 μL样本 |
灵敏度 |
6.04 pg/mL |
检测范围 |
31.25 pg/mL - 2000 pg/mL |
回收率 |
88% - 116% |
平均回收率 |
1.09 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人G-CSF抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的G-CSF与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中G-CSF的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
CSF3 分子靶点信息概述
- 分子名:CSF3, colony stimulating factor 3
- 基因家族:Receptor ligands
- 别名:MGC45931
- 曾用名:GCSF; G-CSF; C17orf33
- 全称:granulocyte colony stimulating factor; pluripoietin; filgrastim; lenograstim; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte)
CSF3 分子靶点综述
粒细胞集落刺激因子(G-CSF)是一种糖蛋白,可刺激骨髓产生粒细胞和干细胞,并把它们释放到血液中。从功能上来说,它是一种细胞因子和激素,是一种集落刺激因子,由内皮细胞、巨噬细胞和许多其它免疫细胞产生。G-CSF刺激中性粒细胞前体和成熟中性粒细胞的生存、增殖、分化及功能发挥。在肿瘤学和血液学上,重组G-CSF用于加速癌症患者化疗后引起的嗜中性白血球减少症的恢复。在白细胞去除前,G-CSF也可用来增加献血者血液中的造血干细胞数量,用于造血干细胞移植。
人 Human CSF3 分子靶点信息
- 分子名:CSF3, colony stimulating factor 3
- 别称:
- C17orf33
- chromosome 17 open reading frame 33
- colony stimulating factor 3 (granulocyte)
- CSF3OS
- filgrastim
- G-CSF
- GCSF
- granulocyte colony-stimulating factor
- granulocyte-colony stimulating factor
- lenograstim
- MGC45931
- pluripoietin
- 基因序列:NCBI_Gene: 1440
- 蛋白序列:UniProtKB: P09919
人 Human CSF3靶点分子功能(预测)
Enables cytokine activity and enzyme binding activity. Involved in several processes, including positive regulation of cytoskeleton organization; positive regulation of intracellular signal transduction; and positive regulation of protein phosphorylation. Predicted to be located in endocytic vesicle lumen; extracellular region; and lysosomal lumen. Predicted to be active in extracellular space. Implicated in several diseases, including allergic cutaneous vasculitis; artery disease (multiple); ischemia (multiple); leukemia (multiple); and lung disease (multiple). Biomarker of COVID-19; bacterial pneumonia; and myeloid leukemia.
操作步骤
文章目录[隐藏]
- ELISA操作常见问题
- 一文掌握ELISA实验显色判断、数据分析及标曲拟合 
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- ELISA通关必备丨样本篇丨不常见样本 
- ELISA通关必备丨样本篇丨常见样本丨细胞 
- ELISA通关必备丨如何选择试剂盒 
- ELISA通关必备丨基础知识 
- 真?假?ELISA试剂盒选择要小心 
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- 查看更多ELISA操作相关问题
ELISA操作常见问题
查看更多ELISA操作相关问题
引用文献
文章目录[隐藏]
- Tumor associated neutrophils promote the metastasis of pancreatic ductal adenocarcinoma 
- Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 
- G-CSF associates with neurogenesis and predicts prognosis and sensitivity to chemotherapy in pancreatic ductal adenocarcinoma 
- Cancer-associated adipocyte-derived G-CSF promotes breast cancer malignancy via Stat3 signaling 
- Tumor-Infiltrating PD-L1+ Neutrophils Induced by GM-CSF Suppress T Cell Function in Laryngeal Squamous Cell Carcinoma and Predict Unfavorable Prognosis 
- A gain-of-function variation in PLCG1 causes a new immune dysregulation disease 
- Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 
- G-Csf Associates with Neurogenesis and Predicts Prognosis and Sensitivity to Chemotherapy in Pancreatic Ductal Adenocarcinoma 
Tumor associated neutrophils promote the metastasis of pancreatic ductal adenocarcinoma 
Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 
G-CSF associates with neurogenesis and predicts prognosis and sensitivity to chemotherapy in pancreatic ductal adenocarcinoma 
Cancer-associated adipocyte-derived G-CSF promotes breast cancer malignancy via Stat3 signaling 
Tumor-Infiltrating PD-L1+ Neutrophils Induced by GM-CSF Suppress T Cell Function in Laryngeal Squamous Cell Carcinoma and Predict Unfavorable Prognosis 
A gain-of-function variation in PLCG1 causes a new immune dysregulation disease 
- 腹腔上清液
Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 
G-Csf Associates with Neurogenesis and Predicts Prognosis and Sensitivity to Chemotherapy in Pancreatic Ductal Adenocarcinoma 
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