EK1F03HS

Human FGF-basic High Sensitivity ELISA Kit检测试剂盒（酶联免疫吸附法）

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¥2,000.00 – ¥3,400.00

  • 分子靶点：FGF2, FGFB
  • 种属：人 (Human)
  • 样本类型：血清，血浆，细胞培养上清及其他生物学样本
  • 检测样本体积：血清血浆：20 μL；细胞培养上清：100 μL
  • 灵敏度：0.04 pg/mL
  • 检测范围：7.81 pg/mL - 500 pg/mL
  • 回收率：86% - 97%

在售SKU:70-EK1F03HS-48, 70-EK1F03HS-96

描述

商品名

Human FGF-basic High Sensitivity ELISA Kit (人碱性成纤维细胞生长因子 高敏 ELISA试剂盒)
检测方法

双抗夹心法
精密度

板内变异系数：3.2% - 5.2%；板间变异系数：3.5% - 6.4%
样本类型

血清，血浆，细胞培养上清及其他生物学样本
检测样本体积

血清血浆：20 μL；细胞培养上清：100 μL
灵敏度

0.04 pg/mL
检测范围

7.81 pg/mL - 500 pg/mL
回收率

86% - 97%
平均回收率

0.9
板式

96孔板，可拆
保存

试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件

4℃蓝冰运输
组分
  • 包被抗FGF-basic 单克隆抗体的96孔聚苯乙烯酶标板
  • 人FGF-basic冻干标准品
  • FGF-basic 检测抗体
  • 标准品稀释液
  • 辣根过氧化物酶标记的链霉亲和素
  • 信号增强剂浓缩液
  • 信号增强剂稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人FGF-basic抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的FGF-basic与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中FGF-basic的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human FGF-basic High Sensitivity ELISA Kit (人碱性成纤维细胞生长因子 高敏 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述人 FGF2, FGFB 靶点信息

FGF2 分子靶点信息概述

  • 分子名：FGF2, fibroblast growth factor 2
  • 基因家族：Receptor ligands; Fibroblast growth factor family
  • 曾用名：FGFB
  • 全称：fibroblast growth factor 2 (basic)

FGF2 分子靶点综述

碱性成纤维细胞生长因子(FGFbasic)，又名bFGF、FGF2或FGF-β，是成纤维细胞生长因子家族中的一员。在正常组织的伤口愈合和肿瘤发展过程中，硫酸乙酰肝素降解酶激活FGFbasic来介导血管生成。另外，人类脂肪细胞可合成和分泌FGFbasic，它的浓度和血液样本中的BMI相关。研究显示低水平的FGFbasic在过度焦虑的发生中发挥重要作用。此外，FGFbasic是人类胚胎干细胞培养基中的重要组成成分，是维持细胞未分化状态的必要因素。FGFbasic与BMP4一起可促进干细胞分化为中胚层谱系。相比于未处理的干细胞，经BMP4和FGFbasic处理过的干细胞通常会分化出更多的成骨细胞和软骨细胞。

人 Human FGF2 分子靶点信息

  • 分子名：FGF2, fibroblast growth factor 2
  • 别称：
    • basic fibroblast growth factor bFGF
    • BFGF
    • FGF-2
    • FGFB
    • fibroblast growth factor 2 (basic)
    • HBGF-2
    • heparin-binding growth factor 2
    • prostatropin
  • 基因序列：NCBI_Gene: 2247
  • 蛋白序列：UniProtKB: P09038

人 Human FGF2靶点分子功能（预测）

Enables several functions, including identical protein binding activity; nuclear receptor coactivator activity; and signaling receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of intracellular signal transduction; and regulation of cell migration. Acts upstream of or within positive regulation of cell fate specification and positive regulation of cell population proliferation. Located in extracellular space. Implicated in several diseases, including Parkinsonism; corneal neovascularization; diabetic neuropathy; gastric ulcer; and impotence. Biomarker of several diseases, including artery disease (multiple); breast cancer (multiple); carcinoma (multiple); eye disease (multiple); and female reproductive organ cancer (multiple).

操作步骤

ELISA操作常见问题

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

ELISA常见类型二 | 竞争法，五分钟搞定！ 

叮！联科向您投递了个ELISA实验操作干货包，请查收~ 

【视频】ELISA实验操作步骤演示视频教程 

ELISA 组织样本的处理—大鼠组织 

【视频】ELISA实验原理与常见问题分析 

查看更多ELISA操作相关问题

引用文献

文章目录[隐藏]


  1. The conditioned medium from mesenchymal stromal cells pretreated with proinflammatory cytokines promote fibroblasts migration and activation 

  2. Human Umbilical Cord-Derived Mesenchymal Stem Cells Ameliorate Skin Aging of Nude Mice Through Autophagy-Mediated Anti-Senescent Mechanism 

  3. Adipose-Derived Stromal Cell-Sheets Sandwiched, Book-Shaped Acellular Dermal Matrix Capable of Sustained Release of Basic Fibroblast Growth Factor Promote Diabetic Wound Healing 

  4. Concentrated growth factor promotes proliferation, osteogenic differentiation, and angiogenic potential of rabbit periosteum-derived cells in vitro 

  5. In Vitro Angiogenic Behavior of HUVECs on Biomimetic SF/SA Composite Scaffolds 

  6. Tanshinone IIA inhibits β-catenin/VEGF-mediated angiogenesis by targeting TGF-β1 in normoxic and HIF-1α in hypoxic microenvironments in human colorectal cancer 

  7. Transverse Tibial Bone Transport Enhances Distraction Osteogenesis and Vascularization in the Treatment of Diabetic Foot 

  8. Utilizing 3D bioprinted platelet-rich fibrin-based materials to promote the regeneration of oral soft tissue 

  9. Advanced-platelet-rich fibrin extract promotes adipogenic and osteogenic differentiation of human adipose-derived stem cells in a dose-dependent manner in vitro 

  10. Bioinspired Collagen Scaffold Loaded with bFGF-Overexpressing Human Mesenchymal Stromal Cells Accelerating Diabetic Skin Wound Healing via HIF-1 Signal Pathway Regulated Neovascularization 

  11. ROS-scavenging lipid-based liquid crystalline as a favorable stem cell extracellular vesicles delivery vector to promote wound healing 

  12. Fabrication of a Coculture Organoid Model in the Biomimetic Matrix of Alginate to Investigate Breast Cancer Progression in a TAMs-Leading Immune Microenvironment 

  13. Ultrasoft bioadhesive hydrogel as a versatile platform for the delivery of basic fibroblast growth factor to repair traumatic brain injury 

  14. 3D bioprinting of multi-cellular tumor microenvironment for prostate cancer metastasis 

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