Human CXCL13/BLC/BCA-1 ELISA Kit检测试剂盒(酶联免疫吸附法)
¥1,600.00 – ¥2,650.00
因产品会迭代升级,具体实验步骤请按纸质版说明书操作
- 分子靶点:CXCL13, BLC, BCA-1
- 种属:人 (Human)
- 样本类型:血清,血浆,细胞培养上清及其他生物学样本
- 检测样本体积:20 μL
- 灵敏度:4.86 pg/mL
- 检测范围:31.25 pg/mL - 2000 pg/mL
- 回收率:87% - 114%
在售SKU:70-EK1105-48, 70-EK1105-96, 人, 趋化, 因子, CXC, 配体, 13
描述
商品名 |
Human CXCL13/BLC/BCA-1 ELISA Kit (人趋化因子CXC配体13 ELISA试剂盒) |
---|---|
检测方法 |
双抗夹心法 |
精密度 |
板内变异系数:1.3% - 6.5%;板间变异系数:2.7% - 4.9% |
样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
检测样本体积 |
20 μL |
灵敏度 |
4.86 pg/mL |
检测范围 |
31.25 pg/mL - 2000 pg/mL |
回收率 |
87% - 114% |
平均回收率 |
0.99 |
板式 |
96孔板,可拆 |
保存 |
试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。 |
运输条件 |
4℃蓝冰运输 |
组分 |
|
检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人CXCL13抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体,经过孵育,样本中存在的CXCL13与固相抗体和检测抗体结合。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中CXCL13的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。
分子信息
CXCL13 分子靶点信息概述
- 分子名:CXCL13, C-X-C motif chemokine ligand 13
- 基因家族:Chemokine ligands
- 别名:BLC; BCA-1; BLR1L; ANGIE; ANGIE2
- 曾用名:SCYB13
- 全称:B-cell chemoattractant; small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant); chemokine (C-X-C motif) ligand 13
CXCL13 分子靶点综述
趋化因子CXC配体13(CXCL13)也称为B淋巴细胞趋化因子(BLC)或BCA-1,高水平表达于人的肝脏、脾脏、淋巴结和肠道中。它选择性地对属于B-1和B-2亚群的B细胞产生趋化作用,通过与趋化因子受体CXCR5作用展现其功能。CXCL13与其受体CXCR5调控淋巴组织滤泡中的B细胞构架。在T细胞中,CXCL13的表达被认为反映了T细胞,尤其是滤泡辅助性T细胞(Tfh)生发中心的起源。因此CXCL13在T细胞淋巴瘤中的表达,如血管免疫母细胞性T细胞淋巴瘤,被认为反映了肿瘤T细胞生发中心的起源。
人 Human CXCL13 分子靶点信息
- 分子名:CXCL13, C-X-C motif chemokine ligand 13
- 别称:
- ANGIE
- ANGIE2
- b cell-attracting chemokine 1
- b lymphocyte chemoattractant
- B-cell chemoattractant
- B-cell-attracting chemokine 1
- B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1)
- B-lymphocyte chemoattractant
- BCA-1
- BCA1
- BLC
- BLR1L
- C-X-C motif chemokine 13
- chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant)
- CXC chemokine BLC
- SCYB13
- small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant)
- small-inducible cytokine B13
- 基因序列:NCBI_Gene: 10563
- 蛋白序列:UniProtKB: O43927
人 Human CXCL13靶点分子功能(预测)
Enables chemokine receptor binding activity; fibroblast growth factor binding activity; and heparin binding activity. Involved in several processes, including antimicrobial humoral immune response mediated by antimicrobial peptide; cell chemotaxis; and regulation of chemotaxis. Located in extracellular region.
操作步骤
文章目录[隐藏]
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引用文献
文章目录[隐藏]
- Cytokine antibody array-based analysis of IL-37 treatment effects in asthma 
- Circulating C-X-C Motif Ligand 13 as a Biomarker for Early Predicting Efficacy of Subcutaneous Immunotherapy in Children With Chronic Allergic Rhinitis 
- An imbalance between blood CD4+CXCR5+Foxp3+ Tfr cells and CD4+CXCR5+Tfh cells may contribute to the immunopathogenesis of rheumatoid arthritis 
- Stromal cells and B cells orchestrate ectopic lymphoid tissue formation in nasal polyps 
- Efficacy and safety of low-dose interleukin-2 in combination with methotrexate in patients with active rheumatoid arthritis: a randomized, double-blind, placebo-controlled phase 2 trial 
- DEX-Induced SREBF1 Promotes BMSCs Differentiation into Adipocytes to Attract and Protect Residual T-Cell Acute Lymphoblastic Leukemia Cells After Chemotherapy 
Cytokine antibody array-based analysis of IL-37 treatment effects in asthma 
Circulating C-X-C Motif Ligand 13 as a Biomarker for Early Predicting Efficacy of Subcutaneous Immunotherapy in Children With Chronic Allergic Rhinitis 
An imbalance between blood CD4+CXCR5+Foxp3+ Tfr cells and CD4+CXCR5+Tfh cells may contribute to the immunopathogenesis of rheumatoid arthritis 
Stromal cells and B cells orchestrate ectopic lymphoid tissue formation in nasal polyps 
Efficacy and safety of low-dose interleukin-2 in combination with methotrexate in patients with active rheumatoid arthritis: a randomized, double-blind, placebo-controlled phase 2 trial 
DEX-Induced SREBF1 Promotes BMSCs Differentiation into Adipocytes to Attract and Protect Residual T-Cell Acute Lymphoblastic Leukemia Cells After Chemotherapy 
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