EK196

Human CXCL1/GRO-α ELISA Kit 检测试剂盒(酶联免疫吸附法)

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¥2,000.00¥3,400.00

因产品会迭代升级,具体实验步骤请按纸质版说明书操作

  • 分子靶点:CXCL1, SCYB1, NAP-3
  • 种属:人 (Human)
  • 样本类型:血清,血浆,细胞培养上清及其他生物学样本
  • 检测样本体积:100 μL(稀释液)
  • 灵敏度:0.10 pg/mL
  • 检测范围:0.78 pg/mL - 50 pg/mL
  • 回收率:73% - 102%

在售SKU:70-EK196-48, 70-EK196-96, EK196


描述

商品名

Human CXCL1/GRO-α ELISA Kit 检测试剂盒(酶联免疫吸附法)

检测方法

双抗夹心法

精密度

板内变异系数:4.7% - 8.4%;板间变异系数:2.8% - 11.0%

样本类型

血清,血浆,细胞培养上清及其他生物学样本

检测样本体积

100 μL(稀释液)

灵敏度

0.10 pg/mL

检测范围

0.78 pg/mL - 50 pg/mL

回收率

73% - 102%

平均回收率

0.92

板式

96孔板,可拆

保存

试剂盒未拆开,4℃保存。已拆开,标准品-20℃保存,其它4℃保存。

运输条件

4℃蓝冰运输

组分
  • 包被抗CXCL1/GRO-α 单克隆抗体的96孔聚苯乙烯酶标板
  • 人CXCL1/GRO-α 冻干标准品
  • CXCL1/GRO-α 检测抗体
  • 标准品稀释液
  • 检测缓冲液(10×)
  • 底物(TMB)
  • 终止液
  • 洗液(20×)
  • 封板膜

检测原理:本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人CXCL1抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本,经过孵育,样本中存在的CXCL1与固相抗体结合。洗涤去除未结合的物质后,加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体,加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后,加入显色底物TMB,避光显色。颜色反应的深浅与样本中CXCL1的浓度成正比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human CXCL1/GRO-α ELISA Kit 检测试剂盒(酶联免疫吸附法) - 标准曲线
标准曲线

分子信息

概述人 CXCL1, SCYB1, NAP-3 靶点信息

CXCL1 分子靶点信息概述

  • 分子名:CXCL1, C-X-C motif chemokine ligand 1
  • 基因家族:Chemokine ligands
  • 别名:SCYB1; GROa; MGSA-a; NAP-3
  • 曾用名:MGSA; GRO1; FSP
  • 全称:melanoma growth stimulating activity, alpha; GRO1 oncogene (melanoma growth stimulating activity, alpha); fibroblast secretory protein; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)

CXCL1 分子靶点综述

趋化因子(C-X-C基序)配体1(CXCL1)是一个小分子量细胞因子,属于CXC趋化因子家族,以前又被称为GRO1致癌基因、GROα、KC和中性粒细胞激活蛋白3 (NAP-3)。在人类中,该蛋白由CXCL1基因编码。CXCL1由人类黑色素瘤细胞分泌,具有促有丝分裂特性,且与黑色素瘤发病机制有关。CXCL1由巨噬细胞、中性粒细胞和上皮细胞表达,有中性粒细胞趋化活性。该趋化因子通过趋化因子受体CXCR2产生信号,进而引发它的作用效果。CXCL1通过抑制少突细胞前体的迁移,从而对脊髓发育起作用。它参与血管生长、炎症、伤口愈合和肿瘤发生过程。小鼠的初步研究表明,CXCL1可降低多发性硬化症的严重程度,可提供神经保护作用。

人 Human CXCL1 分子靶点信息

  • 分子名:CXCL1, C-X-C motif chemokine ligand 1
  • 别称:
    • C-X-C motif chemokine 1
    • chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
    • fibroblast secretory protein
    • FSP
    • GRO-alpha(1-73)
    • GRO1
    • GRO1 oncogene (melanoma growth stimulating activity, alpha)
    • GRO1 oncogene (melanoma growth-stimulating activity)
    • GROa
    • growth-regulated alpha protein
    • melanoma growth stimulating activity, alpha
    • melanoma growth stimulatory activity alpha
    • MGSA
    • MGSA alpha
    • MGSA-a
    • NAP-3
    • neutrophil-activating protein 3
    • SCYB1
  • 基因序列:NCBI_Gene: 2919
  • 蛋白序列:UniProtKB: P09341

人 Human CXCL1靶点分子功能(预测)

Predicted to enable CXCR chemokine receptor binding activity and chemokine activity. Involved in antimicrobial humoral immune response mediated by antimicrobial peptide and killing of cells of other organism. Predicted to be located in extracellular region; specific granule lumen; and tertiary granule lumen. Predicted to be active in extracellular space. Biomarker of asthma; chronic obstructive pulmonary disease; common cold; cystic fibrosis; and hyperhomocysteinemia.

操作步骤

ELISA操作常见问题

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引用文献


  1. Galectin-3 exacerbates ox-LDL-mediated endothelial injury by inducing inflammation via integrin β1-RhoA-JNK signaling activation 

  2. Alteration of Myeloid-Derived Suppressor Cells, Chronic Inflammatory Cytokines, and Exosomal miRNA Contribute to the Peritoneal Immune Disorder of Patients With Endometriosis 

  3. Cancer-associated adipocytes promote the invasion and metastasis in breast cancer through LIF/CXCLs positive feedback loop 

  4. The kinase activity of integrin-linked kinase regulates cellular senescence in gastric cancer 

  5. Cisplatin-stimulated macrophages promote ovarian cancer migration via the CCL20-CCR6 axis 

  6. Deciphering m6A methylation in monocyte-mediated cardiac fibrosis and monocyte-hitchhiked erythrocyte microvesicle biohybrid therapy 

  7. LncRNA MALAT1 suppresses monocyte-endothelial cell interactions by targeting miR-30b-5p and enhancing ATG5-mediated autophagy 

  8. The spike protein of SARS-CoV-2 induces inflammation and EMT of lung epithelial cells and fibroblasts through the upregulation of GADD45A 

  9. An inflammatory checkpoint generated by IL1RN splicing offers therapeutic opportunity for KRAS mutant intrahepatic cholangiocarcinoma 

  10. CSF2 upregulates CXCL3 expression in adipocytes to promote metastasis of breast cancer via the FAK signaling pathway 

  11. 细胞培养上清

    Dedifferentiation-associated inflammatory factors of long-term expanded human hepatocytes exacerbate their elimination by macrophages during liver engraftment 

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